培养瓶

Digestive the well-grown cells with pancreatic enzyme(0.25%), count under the microscope, and make them into one-celled suspension, with a density of 1×105/ml.

将培养瓶中生长状况良好处于对数生长期的前脂肪细胞用0.25%的胰酶消化，显微镜下计数并制成密度为1×105个/ml的单细胞悬液备用。

Ascitic fluid should be inoculated into blood culture bottles at the bedside and examined by microscopy for a neutrophil count.

腹水要在床边接种入血液培养瓶，并要在显微镜下检查嗜中性粒细胞计数。

Ascitic fluid should be inoculated into blood culture bottles at the bedside.

腹水应该在床旁接种入血培养瓶。

Ascitic fluid should be inoculated into blood culture bottles at the bedside.

腹水应该在床边接种入血培养瓶中。

Simple modified differential adherence: monoplast suspension at 8 000/cm2 was incubated in uncoated 25 cm2 flask twice for 12 h +24 h subsequently, the cell suspension was drawn off.

单纯改良差速贴壁：将单细胞混悬液以8 000个/cm2密度种植于无包被的25 cm2培养瓶中。

The virus-rich fluid is then pipetted from the culture bottles and transferred to centrifuge tubes.

然后将富含病毒的液体从培养瓶转移到离心试管中。

The expressions of FⅧr-Ag was positive in BREC. And the W-P body was found in the plasm of the BREC. Conclusions: BREC with high purity can be obtained by using selective culture and coated dishes which are simple and repeatable methods.

选择性培养基的应用，和培养瓶的处理可获得较高纯度的BREC，方法简单且具有良好的可重复性，无需额外步骤来去除BREC中混杂的BRP。

100ML culture flask, nitinol wire, cardiac pacemaker and connecting lead were used to make electric field.

使用一次性100mL培养瓶、镍钛合金金属丝、心脏起搏器和连接导线设计制作电场。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

When deemed absolutely necessary, touch-plate microburners equipped with a pilot light to provide a flame on demand may be used.

老师传授授说在打开培养瓶或培养液之前要用酒精灯的火焰快速的烧一下，目的是消毒，减少污染。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。