培养瓶

Methods Bacteria were diluted different concentration to add Be-tai blood culture bettle in order to observe the bacteria growth state.

将已知细菌稀释成不同浓度，定量加入伯泰血培养瓶，观察细菌生长情况。

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。

In the rattail collagen coated flask or plate, LEC grew well, with 3HTdR incorporation efficiency increasing obviously and cell doubling time being (42.7±3.2) h. In the gel formed by rattail collagen, LEC could form lymphatic vessellike structures.

在鼠尾胶包被的培养瓶中， LEC生长状况良好， 3HTdR掺入率明显增加，倍增时间为（42.7±3.2） h；在鼠尾胶凝胶中，LEC能形成淋巴管样结构。

The outcome is bad in fibronectm-coated plate or 25 cm2 culture flask.

应用纤维连接蛋白和25cm2细胞培养瓶培养的效果不佳。

The outcome is bad in fibronectin-coated plate or 25 cm2 culture flask.

应用纤维连接蛋白和25 cm2细胞培养瓶培养的效果不佳。

Once the cells reached 80% confluency, they were inoculated in culture flask at the density of 1.0×107 cells/L for proliferative culture.

细胞长到80%融合时，以1.0×107 个/L的密度接种于培养瓶中进行扩增培养。

Subsequently, BMSCs were incubated in serum-free L-DMEM, and placed in a plastic culture flask at 1.0×108/L.

按1.0×108 L-1的密度接种到一次性塑料培养瓶中进行培养。

Results The open cycling air pressure control culture system worked effectively, the pressure inside the culture flask can be controlled from 0 to 100 mm Hg.

结果成功组装开放式压力控制培养系统，通过调节进出气流速度，可将培养瓶内压力控制在0～100 mm Hg（1 mm Hg=0.133kPa）之间。

After that the specimens were digested by collagenase for 90 min, the supernatant was collected and centrifuged.

成骨细胞周期和细胞凋亡的检测成骨细胞以 IX 10VInl接种至 25Inl培养瓶，在含 15％胎牛血清的 199培养液中培养 3天。

Methods Thoracic aorta of Wistar rat is obtained, by using a specially made crochet hook, the aorta is over turned, then ligated the two ends, the vascular endothelial cells are wiped off with 0.1% collagen Ⅰ enzyme for 15 minutes.

将大鼠主动脉翻转，两端结扎，用0.1%胶原酶消化血管内皮细胞，剩余血管剪成小块均匀种植于培养瓶底，加入DMEM培养液培养，观察血管平滑肌细胞的生长。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。