培养瓶

High level of Kal was obtained in BMMY medium (pH 7.0) after 96 hours induction of 29?C and 2% methanol, with the highest yield of 14 mg/L in shake flask culture.

阳性表达菌株在BMMY培养基(pH 7.0)中29℃培养，经2％甲醇诱导表达96 h，摇瓶表达量可达14 mg/L。

After further purification by a SuperdexTM 75 HR 10/30 column, finally 6-7 mg of Cd2+-hMT-3 was obtained from 3 L of flask culture with the recovery of about 1.8%.

从摇瓶培养菌液中纯化获得了结合有Cd2+的完整的人金属硫蛋白-3，得率为1.8%。

The results were as follows. At the temperature of 25~30C,initial pH value of 7.5~8.0,alkalinity of NaHCO3 concentration 1700mg/L, NH4+-N concentration of 150mg/L,aerations of 110rpm, cultured 24h,immobilized nitrite bacteria N4s NH4+-N removal efficiency reached 99.47%,accumulative volume of NO2--N was 116.65mg/L.

试验结果表明：固定化亚硝化细菌N_4在温度25～30℃，起始pH值7.5～8.0，摇瓶转速110rpm，碱度为NaHCO_3浓度1700mg／L，初始NH_4~+-N浓度150mg／L，培养24h，NH_4~+-N去除率达99.47％，NO_2~--N积累量达116.65mg／L。

All Mut+and Muts colones were induced respectively with methanol to secrete interesting peptide in shake-flask cultures for four days. After dialysis and lyophilization, the supernate of cultures were identified by SDS-PAGE and Western blotting to find the genetically engineered Pichia pastoris which expressed interesting peptide.

在摇瓶中分别用甲醇诱导Mut~+和Mut~s型转化子表达蛋白4d，取培养产物冻干浓缩，进行SDS-PAGE和Western blotting，分析蛋白的含量及纯度，筛选出能特异表达目的蛋白的基因工程菌。

Among four yeast strains with the capability of decoloring indigo carmine isolated from sludge samples taken from the textile wastewater treatment facility by enrichment cultivate,a strain Y-1 was selected on the basis of excellent dye decolorization.

通过富集培养，从印染污水处理厂污泥中分离出四株靛蓝胭脂红脱色酵母，挑选其中脱色效果较好的酵母Y-1菌株进行摇瓶脱色研究。

Pepper Tree is a vertically integrated Estate winery that owns, farms, harvests all of it's own grapes and crushes, vinifies, ages and bottles all of it's own wines at the winery.

黑椒树酒庄作为一个综合葡萄酒庄园，直接拥有、种植和收获庄园自产葡萄，并在厂内榨汁、酿造、培养、装瓶。

After the microscope through the cultivation of detection, slender hyphae, bifurcation, and short stem hyphae collateral for its health or the alternate on the branch, the branch can continue to branch, forming two, three branch , is the end of vase-shaped branching stems, spores observed liquid spherical sporangium producers body, the formation of conidia oval eggs, smooth-walled.

通过培养后显微镜观察检测，菌丝细长、分叉多，梗为菌丝的短侧枝，其上对生或互生分枝，分枝上又可继续分枝，形成2级，3级分枝，分枝末端即为瓶状梗，孢子液制片观察到球形孢子囊体，分生孢子卵形成椭圆形，壁光滑。

The result of shake culture shows that 8.1 g/L of mycelium and 50% of decolorization rate could be gotten in 5 times diluted waste water by Coriolus versicolors.

摇瓶培养结果表明，云芝对20%的酒精废液的脱色率为50%，生物量为

The effects of metal ions on ethanol tolerance of self-flocculating yeast was studied by using a defined medium in shake flask culture. The observed improvement of zinc ion supplementation on ethanol tolerance promoted the further examination of zinc ion effect on continuous ethanol fermentation. Zinc sulfate was supplemented into the fermentation medium at the concentrations of 0.01, 0.05 and 0.1gL^(-1), respectively.

研究了6种金属离子在合成培养基中对摇瓶培养的自絮凝酵母乙醇耐性的影响，发现锌离子对高浓度乙醇冲击下的酵母细胞活性有保护作用，并进一步研究了乙醇连续发酵过程中锌的添加对自絮凝酵母乙醇耐性的影响。

Cocos yoghurt. The optimum conditions for liquid fermentation of P. cocos polysaccharides were: 150 rpm, 26℃, initial pH 5.5 in medium, inoculating 6% P. cocos strain at 2 d age, shake culture for 7 d and the content in fermentation liquid of about 6.91 mg/ml.

结果］茯苓多糖最佳液体发酵条件为：26℃，150 r/min，培养基初始pH值5.6，接种6%的菌龄为2 d的茯苓菌，摇瓶振荡培养7 d，发酵液中茯苓多糖含量为6.91 mg/ml。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。