培养瓶

The major products are exported (80%), including cell culture plates, bottles of cell culture, cell plate, and the needle-type vacuum filters, and the transfer of the centrifuge tube pumping, such as straw, as well as the excellent quality and highly competitive prices, the majority of customers were endorsed unanimously.

现产品主要出口国外（80%），其中，细胞培养板、细胞培养瓶、细胞培养皿、真空式与针头式过滤器，移液管与抽吸管以及离心管等产品以优良的品质和极具竞争力的价格，受到广大客户的一致认可。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

The corneal fibroblast of rabbit formed a monolayer and had a fibriform arragement after two weeks culture in culture flasks.

眼角膜成纤维细胞在培养瓶中培养 2周后可形成单层；2 4孔板培养 3d后细胞贴壁生长旺盛，10d形成单层。

At the fifth passage, two cell strains were made into cell suspension at l×10^9L^(-1). 0.5 mL was subcutaneously incubated at the inguina of each rat for 8 weeks. Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differeotiated.

体内实验取连续传5代的2个细胞株，分别制备浓度为l×10^9L^(-1)的细胞悬液，于每只小鼠两侧腹股沟处皮下各接种0.5 mL，培养8周体外分化采用无私附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

When the inoculative density of MSCs is l×l0~5cells/ml and the microcarrier Cytodex-3 concentration is 10mg/ml, MSCs cultivated in RWV for five days can expand more than nine times but only four times in T-fiask in one generation. Moreover, the culture parameters show that the media pH value in RWV is 7.4-7.6, and the glucose content is lower but the lactic acid content is higher in RWV than in T-flask.

同时对静态和动态培养的细胞进行形态、pH值、渗透压、葡萄糖、乳酸、流式检测及向成骨细胞诱导，发现旋转壁式生物反应器中培养兔骨髓间充质干细胞时，培养基的pH值在7.4-7.6之间，渗透压变化不大，与细胞在培养瓶中培养比较可见葡萄糖含量低而乳酸含量高。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24，48，72 h，将各时间点细胞上清液及裂解液作为条件培养基，加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

Methods A total of 1 000 samples of blood and body fluid were detected by BACTEC-9120 automated blood culture system with standard aerobic vials, anaerobic vials, and peds plus vials.

用BACTEC-9120全自动血培养仪对1 000份血液和体液标本进行检测，针对不同年龄患者对象选用标准需氧瓶、厌氧瓶和儿童专用培养瓶进行增菌培养。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。