培养微生物

In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量；通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究；将小花棘豆植物单株进行体外真菌培养，研究了菌落和分生孢子的微生物形态学特性，从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区，进行了DNA序列分析与排序；联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果，与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

In this project, in order to develop a designable and controllable mixed microbial culture with intelligent signals, we chose the alkalophilic mixed culture as model system for production of multienzymes consisting of alkaline cellulase and alkaline amylase, and the basic characteristics of the mixed culture was examined.

本项目选择了能够生产碱性淀粉酶和碱性纤维素酶的嗜碱微生物复合体系来生产复合酶，重点研究嗜碱菌复合培养过程的特点及基于荧光蛋白基因快速定量解析复合培养体系的组成菌种及其相互作用的方法论基础。

Quinone profile corresponds to the three most important characteristics of complicate microbial ecosystem, i.e.

传统的平板培养法由于培养条件与实际环境的差异，只能测定 10 %以下的环境微生物[1] 。

Three kinds of BCRC No.51534, 10322 and 10675 would be selected and acted as an experimental sample of Escherichia coli. Results shows that Escherichia coli of No.51534 will appear better performance because the maximum of open circuit voltage, closed current and power density are 1.01V, 22mA and 1342mW/m2, respectively. Concerning the effect of culture time with respect to different phase type on the electricity performance of MFCs, the time points on the intersection between lag phase and logarithmic phase, the middle of point of stationary phase for growth curve of Escherichia coli would appear a good performance of MFCs. In addition, the BCRC No. 51534 Escherichia coli possessing a better performance of MFCs than others would be suggested and applied to further studying. Comparison with the performance of MFCs with respect to electron mediator under different mole number, result shows that electron mediator of methylene blue with 4.63mM would appear a better electricity performance of MFCs than others. Concerning the different material of proton exchange membrane with PTFE-Nafion, Nafion 211, 212 and 117 with respect to the performance of MFCs, result shows that the Nafion 117 applied in MFCs will have a better performance of MFCs than other cases. Finally, the effect of molar concentration on the performance of MFCs would be expected at the studied cases of 0.4M, 0.2M, 0.1M and 0.05M respectively for cathode oxidant, result shows that a good performance of MFCs will happen at the condition of 0.2M. Those observations will be useful to improvement of MFCs in the further study.

於上述电池系统条件下，进行大肠杆菌生长曲线、电子传递介质、质子交换膜、电极与阴极氧化剂对电池电性效能分析；选择编号10322、10675与51534之大肠杆菌为实验菌株，依定量培养之生长曲线取出代表不同时生长特性时期的培养时间，利用亚甲基蓝作为电子传递介质进行实验分析从所测得的电量进行分析，以编号51534之大肠杆菌的微生物燃料电池有最大的开路电压为1.01V及最大闭路电流为22mA；当极化曲线中电压为0.47V、电流为11.4 mA时有最大的功率密度为1342 mW/m2；加以负载有平均工作功率密度294 mW/m2；从生长曲线与电性效能来分析，得知生长曲线的迟滞期与对数期的转变点与静止期的中间点有最佳电性效能表现；对於加入不同莫耳数之电子传递介质methylene blue、neutral red与thionine之电池效能表现，则以加入4.63mM methylene blue电子传递介质的电池有较佳平均功率密度230 mW/m2；另对於质子交换膜PTFE-Nafion、Nafion 211、Nafion 212与Nafion 117之电池效能表现，以Nafion 117质子交换膜的电池有较佳平均功率密度340 mW/m2；对於分析加入不同莫耳数浓度0.4M、0.2M、0.1M与0.05M的阴极氧化剂之电池效能，则以0.2M的阴极氧化剂的电池可得到较佳平均功率密度429 mW/m2。

Some patients have ''monomicrobial bacterascites'' in which cultures are positive but there is normal ascitic neutrophil count.138 Such infections are thought to occur relatively commonly, and the majority are eradicated by the body's natural defence mechanisms (for example, opsonic and complement mediated bactericidal activity).138 139 When a positive culture is obtained, a further ascitic tap with a neutrophil count should be obtained.

有些患者有着&单一微生物细菌性腹水&，其腹水培养为阳性但腹水白细胞计数正常。138这些感染被认为较为常见，大部分可被机体天然的防御机制（如调理素和补体介导的杀菌活性）所根除。138，139当腹水培养为阳性时，应进一步进行腹穿并行嗜中性粒细胞计数。

In this paper, a model of single-species growth in the chemostat on two non-reproducing, perfectly substitutable resources is considered.

讨论单一微生物种群利用两种营养的微生物培养模型。

The mixed microbial populations from the bioleaching plant in Zijinshan Copper Mine is separated and purified,and the identification based on the molecular biological,morphologic and physiologic methods of the microbe depurated and cultured are conducted by polyphasic taxonomy and phylogeny analysis with application of the 16S rDNA sequences and DNA homology technology.

对福建紫金山铜矿生物堆浸系统中的浸矿微生物进行分离纯化，对得到的纯化培养物采用了多相分类与系统发育分析方法，应用16S rRNA基因全序列分析技术，进行分子生物学鉴定、微生物形态性征与生理生化代谢特征鉴定。

Microbial biomass carbon and microbial biomass nitrogen, respiration CO2 and mineralized nitrogen in plant straw fertilized soil were determined under laboratory incubation conditions of constant temperature and moisture based on field above- ground yields of intercropped sweet maize straw and white clover straw.

根据田间甜玉米／白三叶草套种各作物的秸秆产量，在恒温恒湿条件下进行室内培养，探讨秸秆不同方式还田后土壤微生物量碳、微生物量氮、呼吸产生的CO2和矿化产生的无机氮的变化规律。

In the present study, three normal bras, aerobic spore-bearing bacilli, yeast fungus and streptobacterium plantarum, were isolated, identified and screened from intestinal tract of healthy rabbits. After safety and drug sensitivity test, a compound microecomodulator was produced by combining the three floras, each was cultivated separately, and applied to weanling immature rabbits. The effects of the microecomodulator were evaluated by monitoring animal body weight, food consumption and diarrhea incidence rate and recovery rate of the rabbits with diarrhea.

本实验从健康家兔肠道正常菌群中经分离、鉴定，筛选出3种有益菌种：需氧芽孢杆菌、酵母菌和厌氧乳酸杆菌，经安全试验和药物敏感试验合格后，分别发酵培养，制成复合型微生物制剂再应用到断奶幼兔中，观察对幼兔体重、消耗饲料量、腹泻发生率等的影响，同时应用微生物制剂治疗腹泻兔，观察其治疗效果。

Incubation and pot experiments were conducted to determine the effect of the industrial and agricultural wastes of different species and different composting-levels on soil microbial community, to compare the effects of suppressing the bacterial wilt of tomato by these organic matters, and the mechanism of suppressing the disease was discussed from soil microbial ecology point of view in this paper.

本文应用培养和两次番茄盆栽试验研究了施用不同种类和不同腐熟水平的工农业有机废弃物对土壤微生物群落的影响，比较了这些有机物对番茄青枯病防治效果的差异，并从土壤微生物生态角度对番茄青枯病的防治机理进行了初步探讨。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。