培养微生物

After comparison of 30 and 50℃ treatments, AQDS were added into 3 kinds of microorganism biocoenosis from Jiling, Tianjin and Sichuan paddy soil, and it showed that AQDS could accelerate the reduction of Fe 10%-288%, meanwhile, temperature could accelerate the reduction of Fe 6%-17%. In axenic culture treatment of 6 kinds of bacterial strains from Sichuan and Jiangxi paddy soil, the maximum accumulation amount of Fe reduction rate was constant, the maximum reaction rate and ferric reduction rate of ferric strain JX-a08 all increased as temperature rose, which showed that bacterial strain JX-a08 were suitable to 50℃.

在30和50℃培养温度下，来源于吉林、天津和四川的3种水稻土微生物群落添加AQDS可使Fe还原的反应速率常数增加10%~288%，而温度变化的增加幅度仅为6%~17%；对分离自四川和江西水稻土中的6株铁还原菌的纯培养试验发现，菌株JX-a08的Fe最大累积量、还原速率常数、最大反应速率及铁还原率均随培养温度的升高明显增加，表明菌株JX-a08更适于在50℃下生长。

The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r/min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims:(1) controlling the penetrating pressure of the broth in the hypotonicity condition;(2) controlling the pH of the broth between 4.0 and 7.0;(3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure.

本发明以一株粪产碱杆菌为发酵菌种，使用食品工业中常用的蔗糖或葡萄糖作为微生物培养的碳源，以农业生产中常用的化肥作为氮源和无机盐，辅以酵母粉配制微生物生长的培养基，在30℃以1.0vvm、500r/min通风搅拌条件下好氧发酵，发酵过程中流加特殊配制的补料液以达到以下目的：(1)控制发酵液的渗透压处于低渗条件；(2)控制发酵液的pH处于4.0～7.0之间；(3)为微生物生长提供丰富的碳源和氮源等营养物质，经72小时得到威兰胶发酵液，在95℃～115℃下常压干燥得到威兰胶产品。

The American Ginseng ,as the study objects, which grew in different years in Jiling and Beijing, were studied on Dynamics of Soil Microorganisms by separating, culturing, counting and identifying the edaphon among soil of American Ginsengs.

以吉林、北京两个产地不同年限西洋参根际土壤微生物为研究对象，对其土壤微生物进行分离、培养、计数和鉴定来研究土壤微生物区系的动态变化。

Conclusion1 Critically ill patients with indwelling catheters were of the high positive rate of microorganisms,and microorganism spectrum was of special properties;BF formed mainly inside the catheter,the occurrence rate of BF inside the catheter was high and the formation time was short;Changes on catheters and the result of microorganisms was positively correlated with the BF formation.2 Indwelling catheter time was the independent risk factors for the BF formatiom, while age、APACHEⅡscore、sex、urine sugar and the types of antibiotic usage were the potential risk factors for the BF formation;Nurses should perfect the catheter measures for critical ill patients,emphasis on the assessment of risk factors of BF formation,and implement special care in high-risk patients.3 In the early stage of BF formation,irrigation could play the role of eradicative BF effectively;While later,it couldn't,but it can delay the speed of BF formation and maturation;In the choice of washing methods,injection type washing method is superior to infusion.

结论1重危患者留置尿管微生物培养阳性率高，且微生物菌谱具有特殊性；重危患者尿管表面BF主要在尿管内壁形成；内壁BF的发生率高，形成时间短；肉眼观察尿管表面改变情况以及微生物鉴定结果与BF形成呈正相关。2尿管留置时间是重危患者留置尿管表面BF形成的独立危险因素；年龄、APAcHEⅡ评分、性别、尿糖定性以及抗生素使用种类是其可能的危险因素；护理过程中应完善重危患者尿管护理措施，注重对BF危险因素的评估，并对高危患者实施重点护理。3 BF形成早期，冲洗能发挥较好的清除作用；BF形成后期，冲洗无法彻底清除尿管表面BF，但冲洗能延缓BF形成与成熟的速度；在冲洗方式的选择上，注洗式冲洗法对尿管BF的清除效果优于输注式冲洗法。

Basing on utilization of organiccarbon compounds,mixotrophic culture is expected to possess advantages such as aless light requirement,capability of adopting fermentation techniques ofheterotrophic microorganisms and achieving a high cell density.

以对有机底物的利用为基础的微藻混合营养培养具有可降低光的需求、可借鉴已有的异养微生物的培养技术和实现高细胞密度培养等优势，是微藻培养技术的最新发展之一。

The results showed that, Cd6+ stress the Pistia stratiotes root of of the microbes with the impact of microbial flora or physiological groups, training time and the concentration of Cd6+ vary.

结果表明，Cd6+对大薸根系微生物的影响随微生物区系或生理类群、培养时间和 Cd6+浓度的不同而不同；总体来讲，Cd6+对各微生物指标表现出随浓度的变化没有连续性。

Research on rhizosphere microbe is essential for microbial ecology and industrial biotechnology. However, the inability of culturing most rhizosphere microorganisms (around 99%) in the laboratory obviates the research progress.

根际微生物研究对微生物生态学和工业生物技术开发均十分重要，妨碍其研究进展的主要原因是99%的根际微生物物种在实验室中无法成功培养。

This paper studied the characteristics of microbial in raw lignite, isolated and cultured the microorganisms, compared the influences of different types of microbial to improve the briquettes waterproof property.

研究了褐煤原煤中微生物的特征，对微生物进行了分离和培养，比较了不同种类微生物对提高褐煤型煤防水性能的作用。

As shown in the experimental results: as the conductor of petroleum biological degradation, the quantity of microorganism is the important factor of petroleum degradation efficiency, the huge natural capacity of microorganism in soil is its foundation of bioremediation; petroleum density in certain scope do not affect for the degradation efficiency of petroleum, and in the density of 5%, its initial degradation efficiency is higher, however after training 40 days, this kind of advantage change weak gradually, the reason may be the petroleum of high density probably having certain poison role for microorganism; the best ecological condition of petroleum contaminated soil bioremediation is that nutrition material C: N = 60, electron receives body HiOi joining quantity amount to 12 mg/g, water content is 50%, surface activator is anion surface activator of 12 Wan base benzene sulphur sour sodium; the relation of primary and secondary of factor is water content, surface activator, nutrition material and electron receiver.

试验研究结果表明：作为石油生物降解的执行者，微生物的数量是影响石油降解效率的重要因素，土壤中微生物的巨大的自然容量是其生物修复的基础；石油浓度在一定范围内对石油的降解效率并没有影响，并且在5％浓度时，其起始降解效率较高，但是到了培养40天以后，这种优势渐渐变弱，可能还是高浓度的石油对微生物有一定的毒害作用；石油污染土壤生物修复的最佳生态条件为：营养物质C：N为60，电子受体H_2O_2的累计加入量为12mg/g，含水量为50％，表面活性剂为阴离子表面活性剂十二烷基苯磺酸钠；因素的主次关系为：含水量、表面活性剂、营养物质和电子受体。

Sterilization in desinfection chamber and subcultures of collections ;②confection of fermentation media and sterilization technics;③influences to objective produce altering the fermentable conditions ,including the examinations of the parameters about remnant sugar、pH、growth of microorganisms and produce;④separations and distillations of objective produces in fermented broth,the distillation technics of filter press and ion exchange;⑤brief and operation techniques of automatic fermentation tank.

课程内容：本课程主要实验内容：①微生物无菌室灭菌及种子扩大培养技术；②微生物发酵培养基及配制及灭菌技术；③微生物发酵及发酵条件的改变对目的产物代谢的影响，包括菌体生长、pH、残糖和产物等发酵过程中各项参数的检测；④发酵液中目的产物的分离及提纯，板框压滤离子交换提取技术；⑤自动发酵罐的简介及操作技术。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。