培养微生物

5 kinds of paddy soil treatments were studied, then each microorganism biocoenosis was extracted or bacterial strain was dissociated. Artificial synthesis ferric oxide was considered only electron acceptor, which inoculated soil leaching liquor or bacterial strain with reduction function in mineral salt culture system under anaerobic constant temperature condition. After different temperature treatments towards leaching liquor, temperature was controlled and AQDS added into culture system in order to discuss the effect of temperature and AQDS on ferric oxide microorganism reduction process.

采用5种水稻土为供试材料，分别提取微生物群落或分离铁还原菌株；以人工合成氧化铁作为惟－电子受体，在无机盐培养体系中接种土壤浸提液或具有铁还原功能的菌株，厌氧恒温培养；通过对接种液的不同温度处理(40， 50， 60， 70℃)、对培养温度的控制（30和50℃）以及向体系中添加AQDS，探讨温度及AQDS对氧化铁微生物还原过程的影响。

The main results are: construction of Tarim desert highway shelter-forest promoted soil microbial development, and with increase of plantation time of the shelter-forest, the quantity, fatty acid diversity index of soil microbe and diversity index of bacterial DNA segment enhanced notably. As to soil microbial community structure, bacteria are dominant population with amounting for over 80% of total soil microbe, and fungi is not more than 1% of total soil microbe, but soil microbial community structure was different among three soil layers. The conclusions from study methods of conventional culture, biological marker and molecule biology were almost identical, which illuminates that construction of Tarim desert highway made soil microbial activity enhance and so promote nutrient cycle and utilization of Tarim desert highway shelter-forest land.

结果表明，塔里木沙漠公路防护林的建设促进了风沙土土壤微生物的发育，随着防护林定植年限的增加，土壤微生物数量、脂肪酸和细菌DNA片段的多样性指数明显增大；土壤微生物区系组成中，细菌是优势类群，占微生物总数80%以上，而真菌很少，不到微生物总数1%，但在不同土层间有所差异；传统培养法与现代生物标记和分子生物学方法对塔里木沙漠公路防护林地土壤微生物多样性的研究结果基本一致，说明塔里木沙漠公路防护林的建设使林地土壤生物活性有所增强，有利于林地土壤养分循环与利用。

Furthermore, several bacteria genera are anaerobic or facultative anaerobic, and therefore cannot form pure cultures by ordinary streak plate method. Combination of well developed classical anaerobic microbiological techniques with modern molecular techniques, along with dynamic understanding of growth status and mode of odor production by different bacteria genera is critical for successful future studies.

此外， 猪粪尿排泄物中很多微生物是厌氧或兼性厌氧菌，无法通过普通的平板划线法而获得纯培养，今后应将传统的微生物厌氧培养方法与现代微生物分子生物学技术结合，动态了解和掌握不同菌群的生长状态及在恶臭产生过程中的作用。

Culturable microorganisms provide very limited information on soil microbial diversity,because only 0.1%-10% of soil microorganisms can be cultured under conventional laboratory conditions.

传统的基于培养的研究方法只能反映土壤中少数(0.1%～10%)微生物的信息，而大部分微生物目前还不能培养，因而这部分微生物资源尚难以被有效地开发利用。

The changes of Cr and Fe concentration were measured during Co-culture experiment and slurry experiments. The microbial reducing ability of different chromate concentrations and the effect of chromate on dissimilatory iron reduction in paddy soil were investigated.

通过接种土壤微生物的混合培养及土壤泥浆培养试验，测定了厌氧培养过程中Cr和Fe浓度的变化，研究了不同浓度铬酸盐的微生物还原能力及不同浓度铬酸盐对水稻土中氧化铁还原的影响。

The Co-culture experiment and slurry experiments were applied to two paddy soil samples, which from Sichuan and Jiangxi provinces. The concentration varying of Crand Fe was measured in the course of incubation to study the chromate reducing capability of microbe and effect of chromate on dissimilatory iron reduction .

采用四川和江西水稻土为供试土壤，通过接种土壤微生物的混合培养及土壤悬液培养试验，测定厌氧培养过程中Cr和Fe的浓度变化，研究了不同浓度铬酸盐的微生物还原能力及不同铬酸盐浓度下氧化铁的还原能力。

The results show that Cd2+ for Pistia stratiotes and Myriophyllum spicatum L root-like effects of microbial flora with the microorganism or physiological groups, training time and Cd2+ concentration varies; Micro-organisms under Cd2+ stress would have two distinct effects: First, the growth of micro-organisms are not suited to reduce the number or extinction; Second, the growth of micro-organisms adapt to an increase in the number or the accumulation.

结果表明，Cd2+对大薸和穗状狐尾藻根系微生物的影响随微生物区系或生理类群、培养时间和 Cd2+浓度的不同而不同；受重金属镉胁迫后微生物会产生两个明显的效应：一是不适应生长的微生物数量会减少或灭绝；二是适应生长的微生物数量会增加或积累，在低浓度下，重金属对微生物数量一般有刺激作用，而在高浓度下一般为抑制作用，也有表现出刺激作用。

According to the above study result, the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable. The close relations between abundance of bacteria and nutrients were found. The correlation between heterotrophic bacteria and COD was 0.949. The correlation between organic phosphate bacteria and TP was 0.815. The correlation between nitrosobacteria and NH3-N was 0.909. The correlation between disnitrifier bacteria and TN was 0.653. These inneglectable great factors effecting on phosphorus-uptake and phosphorus-release of phosphorus accumulating organisms are the influent COD、the concentration of DO、the sludge retention time、the temperature. The diversity of organic phosphate bacteria population in the SUFR reactor was complex and there is no phosphate bacteria taking the absolute superiority in quantity. The near-native pure culture method powerfully complement the traditional pure culture technique and enrich culture technique. The sludgy bioactivity is very good in whole SUFR reactor. By the quality of being biochemistry, the anaerobic phase is higher than anoxic phase and the anoxic phase is higher than aerobic phase of SUFR.

通过以上试验内容研究发现，SUFR 系统中的微型动物种群较多，微生物生态系统稳定；系统中微生物的数量与营养盐的含量密切相关，其中总异养菌与COD的相关系数r 为0.949，有机磷细菌与TP 的相关系数r 为0.815，亚硝化菌与NH4+-N的相关系数r 为0.909，反硝化菌的数量和TN 的相关系数r 为0.653；污水生物除磷工艺中进水COD 的浓度、DO 浓度、泥龄的长短、温度都是影响聚磷菌释磷及吸磷效果的不可忽视的因素；SUFR 系统中的磷细菌呈现种群多样化的趋势，没有占绝对优势数量的磷细菌；微孔滤膜近自然培养法是对传统纯培养技术和富集培养技术的有力补充；整个SUFR 反应器系统中污泥的生物活性很好，就可生化性来讲，厌氧反应器大于缺氧反应器大于好氧反应器；按照动力学方程式求出的细胞生长动力学特征值和SUFR 脱氮除磷系统工艺的实测值结果基本吻合。

Summarization of the literatures in this research field are made comprehensively. The experiments are made as follows: H〓S and CH〓SH metabolizing micro-organisms are incubated and enriched in the bottles, into which quantitative inorganic nutrient salt and phosphate buffer are added.

研究以全面的文献综述为基础；微生物驯化培养采用液相硫化物加富培养筛选的方法；微生物包埋方案比较了多种包埋主剂、助剂和交联条件；固定化微生物颗粒填充滴滤床实验探索了驯化液pH值控制、循环液流量、处理负荷等因素对生物脱臭的影响。

Effects of complex microbial fertilizer on tomato bacterial wilt and soil microbial diversities were studied by agar media test and BIOLOG GN micro-plate culture.

采用微生物培养和B IOLOG GN2微平板培养，研究了施入自制的复合微生物菌剂对番茄青枯病和土壤微生物多样性的影响。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。