培养微生物

Bacteriology a nutrient substance that is used to cultivate micro-organisms.

一种用来培养微生物的营养物质。

Recently, with the application of the metagenome technique, genetic fingerprinting technical etc, it becomes more and more popular.

现代分子生物学技术和基因组学的深入研究，为开发环境中的未可培养微生物提供了新的研究方法和机遇。

Content: Pure cultivation of microbes and microtechnique, cell structure and function of microorganism, nutrition of microbes, metabolism of microbes, reproduction of microbes and its control, microorganic genetics and gene engineering, microbial ecology, evolution, phylogenesis, classification, microbial industry and products.

课程内容：微生物的纯培养与显微技术；微生物的细胞结构与功能；微生物营养；微生物代谢；微生物生长繁殖及控制；微生物遗传及基因工程；微生物的生态；微生物进化系统发育和分类鉴定；微生物物种多样性；微生物传染与免疫；微生物工业及产品。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Previous research has suggested that the true producers of numerous natural products isolated from marine invertebrates were the microbial epibiont and symbiont which are deemed as not-yet-cultivated microbe.

对海洋无脊椎动物天然产物的研究表明，很多种活性物质的真正生产者是与其共生或附生的未培养微生物。

Form、structure、characterization and function of prokaryotic、eukaryotic and molecular organisms;②nutrition and cultivation techniques of microorganisms;③physiological and metabolizable production of microorganisms;③theory of genetic variation and microbic breeding technique of microorganisms;⑤ecology and environmental protection of microorganisms;⑥Infection、 Immunity and biologics of microorganisms;⑦classification and identification of microorganisms and the international Code of Nomenclature of microorganisms.and microbial high-techs developing on these as the fundament of life sciences.

主要讲授：①原核微生物、真核微生物及分子生物的形态、结构、特点和功能；②微生物的营养和培养技术；③微生物生理及代谢产物；④微生物遗传变异理论和育种技术；⑤微生物生态和环境保护；⑥微生物传染、免疫和生物制品；⑦微生物分类鉴定和国际命名法则，以及在此基础理论上发展起来的微生物高新技术，是当代生命科学研究的基础。

METHODS: The suspected pathogens and unculturable phylotypes in subgingival oral flora from chronic periodontitis sufferer were detected by PCR based on species-specific ribosomal 16S rRNA gene. A KAPPA calculation was used to analyze the relationship between any two bacterial species.

使用基于16S rRNA基因检测的PCR法，对慢性牙周炎患者龈下菌斑中牙周可疑致病微生物和代表性未获培养微生物进行检测，通过KAPPA检验分析任意两种细菌对间的关系。

D、B. f/F. n combinations accorded with other's conclusions. Symbiotic relationship of AU126/P. n、AU126/F. n、AU126/X112 showed the unculturable phylotypes's roles in the subgingival oral flora.

d、B.f/F.n细菌组合的共生关系与文献报道一致，AU126/P.n、AU126/F.n、AU126/X112细菌对间的共生关系显示了未获培养微生物在龈下菌群中扮演的角色。

Results show that microbial biomass carbon and microbial biomass nitrogen in fertilized soil peak in the first several days, and then decline. Incubating both sweet maize straw and white clover have the highest yield, maxima of 529.57 mgkg^(-1) and 75.50 mgkg^(-1) respectively. They also release respired CO2 the most among all treated soils. The best mineral nitrogen release is in white clover incubating, with a maximum of 29.81 mgkg^(-1) under a 26-day culture, and then keeping 1.60 times the control and reaching 2.48 times at 80-day culture. Sweet maize incubating has the least mineral nitrogen release. It is 13%~53% of the control under a 26-day culture, with a maximum of 7.51 mg kg^(-1). Soil nitrogen mineralization is insignificant under sweet maize incubating with urea in short-term.

研究发现，各施肥处理的土壤微生物量碳、微生物量氮均在培养前期出现峰值，后期平稳降低；甜玉米桔秆和白三叶草绿肥同时还田的土壤微生物量碳、微生物量氮在各培养时期均最大，峰值分别达529.57 mgkg^(-1)，和75.50 mgkg^(-1)，土壤呼吸产生的CO2最多；白三叶草绿肥单独还田有利于土壤无机氮的释放，培养第26d无机氮达到最大值，为29.81 mgkg^(-1)，之后一直在对照处理的1.60倍以上，第80d达到2.48倍；甜玉米秸秆单独还田不利于土壤无机氮的释放，培养的第26d至结束，甜玉米秸秆处理的无机氮为对照的13%~53%，最大为751 mgkg^(-1)；甜玉米秸秆配施尿素，短期内不利于土壤无机氮矿化。

Results show that microbial biomass carbon and microbial biomass nitrogen in fertilized soil peak in the first several days, and then decline. Intercropping Incubating both sweet maize straw and white clover have the highest yield, maxima of 529.57 mg·kg-1 and 75.50 mg·kg-1 respectively. They also release the most respired CO2 the most among all treated soils. The best mineral nitrogen release is in white clover intercroppingincubating, with a maximum of 29.81 mg·kg-1 under a 26-day culture, and then keeping 1.60 times the control and reaching 2.48 times at 80-day culture. Sweet maize incubatingintercropping has the least mineral nitrogen release. It is 13%~53% of the control under a 26-day culture, with a maximum of 7.51 mg·kg-1. Soil nitrogen mineralizatiomineralization is insignificant under sweet maize incubatingintercropping with urea in short-term.

研究发现，各施肥处理的土壤微生物量碳、微生物量氮均在培养前期出现峰值，后期平稳降低；甜玉米秸秆和白三叶草绿肥同时还田的土壤微生物量碳、微生物量氮在各培养时期均最大，峰值分别达529.57 mg·kg-1和75.50 mg·kg-1，土壤呼吸产生的CO2最多；白三叶草绿肥单独还田有利于土壤无机氮的释放，培养第26 d 无机氮达到最大值，为29.81 mg·kg-1，之后一直在对照处理的1.60倍以上，第80 d达到2.48倍；甜玉米秸秆单独还田不利于土壤无机氮的释放，培养的第26 d至结束，甜玉米秸秆处理的无机氮为对照的13%～53%，最大为7.51 mg·kg-1；甜玉米秸秆配施尿素，短期内不利于土壤无机氮矿化。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。