培养

Human lung adenocarcinoma cells at logarithmic growth phase were cultured in vitro for 24 hours,and then were cocultured with arsenic trioxide (AS2O3 ) in the dosages of 075,10,15 and 20?

以体外培养的人肺腺癌细胞株细胞为研究对象，选用对数生长期细胞，培养？

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

The concepts and methods of the Quality-Pedagogy are the following:Self-confidence cultivation, expecting value promotion, progressive elements development, advantages and fortes presentation, different keys to different lock in consideration of the present and the fuiure possibilties, realizing the inclination of thd pedagigical concepls, affections, objectives,and...

面向&差生&的教育观念和方法主要有：培养自信心，提高期望值；发展进步因素，发挥优势与特长；按现有和可能发展水平因材施教；抓最佳时机，实现教育观念、感情、重点和方法的倾斜；培养&情商&因素，引导自我教育

SWEET KELP POWDER IN COSMETIC, SKIN AND HAIR CARE INDUSTRIESSWEET KELP POWDER products are widely adopted as ingredients in making natural cosmetics, skin and hair care products for people with normal skin, dried skin, damaged skin and aging skin, and hair damaged, scalp protection in multiply applications such of pH balance, secretion balance, nurturing, moistening, activating, purification, regeneration and rejuvenation and sunburn protection.

甜海带粉化妆品、护肤护发甜海带粉制品业的广泛采用，使天然成分的化妆品，皮肤和头发护理产品，为残疾人正常皮肤，皮肤干燥、皮肤老化、皮肤损害、而头发受损，保护头皮的pH值乘以申请这种平衡，平衡分泌、培养、滋润、活化、净化、再生与复兴、炙保护。

Methods: BMSCs were harvested from the femurs, tibias and humerus bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肱骨中分离BMSCs进行纯化、培养。

Methods BMSCs were harvested from the femurs, tibias and humems bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肚骨中分离BMSCs进行纯化、培养。

In present study, we used the adult rabbits articular chondrocytes as the object of study, we researched the effect of TGF-β1 to promote chondrocytes proliferation and preserve the synthetise of special stroma in order to provide theortically and clinically basis of culture and proliferation in vitro of a-dult people articular chondrocytes.

材料与方法 1。成年兔关节软骨细胞分离、培养：取18月龄的成年日本大耳白兔1只，处死，片状切取关节软骨，解剖刀将软骨切成1.0mm~3大小碎块，消化，过滤，离心，收集关节软骨细胞，加入DMEM培养液(含20％胎牛血清、青霉素100U／ml，链霉素100U／ml)，调整细胞密度为2.5×10~5／ml，置于37℃，5％CO_2孵育箱内培养。

Responsibilities: Independently perform audit work for assigned project, include: planning, field work and audit report Raise recommendations according to project audit findings. Monitor the progress of regional stocktake,...

。。。督办理有关审计要求的一切事宜。4）培养、发展优秀财务人员，提高财务管理人员素质及专业能力。5）根据要求时间，准确完成各项规定报表及其他不定期报表。6）编制当日销售收款、费用报销等财务凭证，负责相应的登记账；协助出纳核对库存。。。

Methods The samples from oil-containing wastewater were separated and cultured to screen oil degradation bacteria. The strain was identified through sequencing its 16S DNA and BLAST in GenBank combined with routine biochemical reactions of the bacteria.

含油脂废水中取样，通过分离、培养，筛选出以油脂为唯一碳源和能源并能分解油脂的菌株，对其基因组16S DNA测序，在核酸数据库中进行BLAST比对，并进行行生化反应，进行菌种的鉴定。

Pepper Tree is a vertically integrated Estate winery that owns, farms, harvests all of it's own grapes and crushes, vinifies, ages and bottles all of it's own wines at the winery.

黑椒树酒庄作为一个综合葡萄酒庄园，直接拥有、种植和收获庄园自产葡萄，并在厂内榨汁、酿造、培养、装瓶。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。