型录

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

In vitro transcripts from RNA5 wild type clone were co-inoculated into Tetragonia expansa with the RNAs of Hu3 isolate containing RNA1, 2 and 3. Following RT-PCR and Northern blotting results shows RNA5 could be biologically replicated in the leaves. The virus concentration of the isolate containing RNA5 in T. expanxa was higher than that of the isolate Hu3 evaluated by ELISA detection.

野生型RNA5的体外转录物与含Hu3(RNA1+2+3)分离物的总RNA混合接种番杏叶片的RT-PCR和Northern blot检测结果都表明，野生型RNA5能够在接种寄主体内复制，并且ELISA检测证明含有RNA5的番杏病叶中病毒浓度高于不含RNA5病叶的病毒浓度。

However, the transgenic lines overexpressing the truncated ST12 conferred enhanced salt tolerance, and had the normal phenotype and seeds in the tasselling time.

然而，过表达ST12的截短的转录本的转基因株系表现出耐盐的表型，且抽穗期表型和结实率正常。

From the present study, it has been demonstrated that CD44 is cleaved subsequently by MT1-MMP and Presenilins in PC-3 Cells, and in turn causes the release of the CD44ICD. CD44ICD translocates into nuclear and may regulate the tumor cells malignant phenotype via NF-κB pathway which plays a critical role in the malignant progression of prostate cancer, interruption of this pathway might contribute to the inhibition of tumor growth and invasion.

本研究表明：在PC-3细胞中CD44分子分别受MT1-MMP和Presenilin1/2的作用，最终导致CD44ICD从膜结合型CD44ΔE的水解释放并转移进核，并可能通过调控NF-κB等转录因子，影响肿瘤细胞的恶性表型。

Cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A.

接著并以活性为导向的分离策略，从樟芝子实体乙醇抽出物中分离出具抗发炎活性成分antrocamphin A，续利用LPS诱导小鼠巨噬细胞（RAW 264.7）产生发炎的模式，解析antrocamphin A之抗发炎机制，结果证实antrocamphin A确可有效抑制一氧化氮自由基和前列腺素的生成，透过蛋白质表现分析得知，antrocamphin A之抗发炎活性是经由抑制一氧化氮生成酵素和第二型环氧酵素的mRNA表现，进而抑制了一氧化氮生成酵素、第二型环氧酵素和核转录因子-κB等酵素之表现。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

The different types of bcr/abl splicers were present in CML patients in phases and there may be a certain relevance of splicing and hematological parameters.

结论不同阶段CML患者体内大都存在着不同的bcr/abl剪切型转录本，不同剪切型的存在具有一定的血液学相关性。

Results As shown in Western blot analysis, the presence of microsomes did not alter the molecular weight of TM-TNF , but it did result in the cleavage of the IL-2 signal peptide from S-TNFm,suggesting that the leader sequence of TNF might differ from the signal peptide of typical secretory protein in that it seemed not to have undergone cleavage during translation in the rough-faced endoplasmic reticulum. Further enzymatic analysis revealed that TM-TNF was converted into S-TNF through the effect of certain metalloproteinase. Conclusions These results suggest that the mechanisms of TNF-α production may be as follows: Activation of TNF-α producing cells by LPS leads to augmented transcription/translation of TNF-α gene, resulting firstly

实验结果提示17×103 S-TNF产生机制可能是：TNF产生细胞经LPS等激活后，导致TNF基因转录翻译增加，首先形成26×103 TNF，并借助其信号肽疏水氨基酸部分将之&锚定&在细胞膜，成为跨膜型TNF，介导细胞与细胞之间的生物学效应；在某些蛋白酶作用下，可将mTNF的&信号肽&切除，产生17×103分泌型TNF，释放至体液中，在局部或全身发挥作用。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。