型录

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

Methods: 9 cases of exogenic OVC、6 cases of cystoid and infiltrative OVC、15 cases of well-differentiation OSCC and 15 cases of poor-differentiation OSCC were selected; two specimens, which were carcinoma tissues and the sur...

方法取9例外生型口腔疣状癌、6例囊肿／浸润型口腔疣状癌、15例高分化鳞癌、15例低分化鳞癌，应用半定量逆转录多聚酶链式反应方法检测口腔疣状癌和口腔鳞癌中OPN mRNA的表达水平，应用免疫组织化学法检测上述标本中OPN的表达及分布，判断差异。

TGF-βbinds to a heteromeric complex of type I and type II receptors. The constitutively active type II receptor phosphorylates and activates the type I receptor which phosphorylates Smad2 and Smad3 (receptor-associated Smads). Receptor-associated Smads bind the common mediator Smad4 and are translocated to the nucleus, where they associate with DNA-binding cofactors to modulategene transcription.

TGF-β与其效应细胞表面的Ⅱ型受体TβRⅡ和Ⅰ型受体TβRⅠ形成的异源二聚体结合，相继激活TβRⅡ和TβRⅠ，活化的TβRⅠ再进一步活化Smad2和Smad3，磷酸化的Smad2/3必须与Smad4结合形成复合物，才能进入细胞核，调节靶基因的转录[6]。

In order to analyze proteins which may interact with AIF, interceptive AIF gene (mitochondrial localization sequence, MLS, was deleted) which was amplified from the total mRNA extracted from hepatoma HepG2 cells by reverse transcriptase polymerase chain reaction, was inserted into pcDNA3.1 plasmid.

为了筛选可能与截断型AIF存在相互作用的蛋白质，探讨截断型AIF诱导细胞凋亡的机制，用逆转录PCR扩增剪切掉线粒体定位信号的人AIF基因片段并克隆入pcDNA3.1载体，导入肝癌HepG2细胞。

The result showed that Insulin capsule could increase the insulin sensitivity of model rat of insulin resistance of typeⅡdiabetes mellitus by decreasing its blood sugar,decreasing its avoirdupois,improving its lipodystrophy and hemodynamics , increasing its GLUT4 translocation of skeletal muscle cell,increasing expression level of GK in liver cell, increasing expression level of PEPCK and decreasing level of TNF-αexpression in adipose tissue.

结果表明，胰苏灵胶囊可使2型糖尿病IR模型大鼠的血糖降低、体重减轻、脂质代谢异常得到改善、血流变异常得到改善；能使模型大鼠的脂肪组织TNF- a的mRNA表达水平降低，能使模型大鼠骨骼肌细胞的GLUT4的转位增加，能增加肝GK表达，能诱导PEPCK的转录。通过以上作用，胰苏灵胶囊可使2型糖尿病IR大鼠模型的IR得到改善。

The RNAs synthesized in PK15 cells of GD and BF strain, representative strain of PCV2 dominant genotype (CHN-2H) and nondominant genotype (CHN-2A) respectively, were characterized by Northern blot and RT-PCR.

采用Northern blot和RT-PCR技术对PCV2优势基因型(CHN-2H)的代表毒株和非优势基因型(CHN-2A)的代表毒株进行了PK15细胞培养的基因转录分析。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

The results showed that: 1 under the hydroponic culture established in this research, the experimental wheat plants grew well andperformed the typical architecture responses when exposed in Pi deprivation. Hemi-qantitative RT-PCR revealed that the transcriptional level of known P response gene changed dramatically; 2 In general the length of wheat root axis and the root/ shoot ratio arose when the Pi supply was deprived, however, the change models differed among different wheat genotypes. The investigation on their molecular basis would offer the useful clue for seeking high phosphorus efficiency protoplasma and gene resources of wheat.

结果表明：1在研究建立的小麦水培体系中，小麦植株生长正常并对磷饥饿胁迫条件做出发育形态建成水平的反应；半定量RT-PCR分析显示在此体系中小麦磷响应基因也在转录水平上产生显著变化；2在水培条件下不同基因型小麦对磷饥饿胁迫的反应总体上表现在根轴长度和根冠比有所提高，但不同基因型小麦的反应特点有差异；对这些反应特征的分子机理的深入分析可为有效筛选磷高效小麦新种质以及发掘磷高效基因资源提供线索。

Methods:The level of E2 was evaluated by Progesrone Serozyme,the expression of ER was tested by Immunohistocheminal assay,ER gene expression was tested by RT-PCR in 35 cases with mammary hyperplasia of stagnation of Liver-qi type,15 cases with mammary hyperplasia of disorder of thoroughfare vessel and conception Vessel type and 30 cases of healthy people were taken as control group.

分别用磁分离酶联免疫测定法、免疫组化法和逆转录—聚合酶链反应法，检测35例肝郁气滞型乳腺增生病患者及15例冲任失调型患者和30例正常人黄体期血清雌激素水平（E2 ）、乳腺组织雌激素受体及受体基因的表达。

EF116490. Characterization and comparison of this sequence with mouflons, goat, cattle and European bison orthologues were also conducted. Results showed that: 1 The 5'-flanking region contained many potential transcriptional factor binding sites such as those for C/EBPb, C/EBP, SP1, Cap, USF, HFH-2, HNF-3b, and Oct-1, which might have an important effect on transcription activation and regulation as well as tissue-specific expression.

EF116490，在分析其序列结构特征的基础上与GenBank中摩弗伦羊、山羊、普通牛、欧洲野牛进行了比较基因组学和系统进化研究，结果表明：(1)欧拉型藏绵羊GHR基因启动子P1区存在C/EBP、C/EBPb、SP1、Cap、USF、HFH-2、HNF-3b、Oct-1等多个潜在的转录因子结合位点，可能与GHR基因的转录调控和起始以及特异表达有关。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。