型录

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

The lack of liver and kidney type PBMC in patients with alopecia areata Thl/Th2 type cytokine gene expression imbalance, presented to Th1-type cytokines and transcription factor T-bet gene over-expression of Th1-type response characteristics, may be involved in the pathogenesis of alopecia areata, alopecia areata may be insufficient evidence of liver and kidney-shaped one of the micro-nature.

肝肾不足型斑秃患者PBMC中Thl/Th2型细胞因子基因表达失衡，呈现以Th1型细胞因子和转录因子T-bet基因过度表达为特征的Th1型反应，可能参与了斑秃的发病机制，也可能是斑秃肝肾不足证型的微观本质之一。

In fish and amphibia, the transition from maternal gene transcription to zygotic transcription happens during the time of midblastula formation, this stage is named by midblastula transition, at this period, embryo cell cycle composition changes, it is the first time to G1 time appearance, the cell cycle timelimit lengthens and the cell division synchronism loses, the large quantities of zygotic gene transcription starts.

鱼类和两栖类由母型调控向合子型调控过渡发生在中囊胚形成阶段，称为中囊胚过渡(midblastula transition，MBT)，胚胎细胞周期时相的组成在此期间发生变化，首次出现G1期，周期时限延长，细胞分裂的同步性丧失，大批合子型基因活化转录也在此时开始。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

The thesis mentioned that the conformation of lipase molecular can be induced by substrate to be an activated one in organic phase through constructing a water-in-oil micro-emulsion system. The activated conformation was recorded using high cross linking degree polymer. The effect of inducement on the activity of recorded lipase polymer was studied, the catalyzed property of recorded lipase polymer in water phase was investigated, and the fluorescence spectrum of lipase solution and recorded lipase polymer under different kinds of denaturalized circumstance were analyzed.

本文提出通过构建油包水型微乳体系，使酶分子能够在有机相中被反应底物诱导出活性构象，利用高交联度聚合物对该构象进行刻录，比较了底物诱导对刻录酶活性的影响，考察了刻录酶在水相中催化特性，并对脂肪酶溶液和刻录酶聚合物在各种变性条件下的荧光光谱进行分析。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关，植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性，为了验证它在体内的功能，本实验构建了过量表达载体和沉默载体，利用花浸染法把农杆菌携带的双元表达载体，pCAMBTA3301： at3g51960，pCAMBTA3301： at3g51960 Promoter和pCAMBTA3301： at3g51960-GFP及pCAMBTA3301： at3g51960 cDNA转入拟南芥，通过多次筛选已经获得了转基因拟南芥的纯合植株，对获得的转基因植株的表型分析发现，在过量表达at3g51960的转基因拟南芥中对胁迫的抗性有一定程度的变化，因此推测at3g51960可能参与植物的抗性调节。

It is now generally accepted that interactions between ligand-receptor complexes and cofactors are essential for AR function, selectivity, sensitivity, and may contribute to the progression of prostate cancer.

我们应用PKC激动剂PMA对AR进行的研究表明，PMA对野生型AR和AR突变体均有基本相同的配体依赖性转录激活作用，并且还与DHT一起，对野生型AR及AR突变体的转录激活起协同的作用。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。