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The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合表达载体pTYB11-EFE融合表达,对诱导剂IPTG的浓度和诱导温度进行了优化,通过降低诱导温度使产物由包含体变成可溶性融合产物;利用融合分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#,对诱导时间进行了优化,获得了较高的表达量,但发现产物没有明显的分泌表达,绝大部分是以可溶形式存在于胞内。

Chinese petrous picture are classified into four types: noumenal symbols,simulative symbols,symbolic symbols and behavioral symbols.

中国岩画的象征分为四种类:本体象征、拟象象征、符号象征和动作象征

The lumped kinetic model of monoterpene, abietic type resin acid, pimaric acid type resin acid, p-cymene, hydro-monoterpene, dehydro-abietic acid, hydro-abietic type resin acid, and hydro-pimaric acid type resin acid was established based on the structural groups and approximate reaction kinetic theory according to the fundamental principles of the lumped method.

在消除内、外扩散影响的条件下,在线跟踪反应产物并用气相色谱法测定反应体系组成随时间的变化,构造了Pd/C催化剂上松脂分子间氢转移集总反应网络,建立了包含单萜烯、枞酸树脂酸、海松酸树脂酸、对伞花烃、氢化单萜烯、脱氢枞酸、氢化枞酸树脂酸和氢化海松酸树脂酸等复杂反应体系的集总动力学模,并估算了模参数。

The gold deposit types are com plicated,can be divided into five types;hydrothermal breccia gold deposit,similar beded disseminated gold deposit in limestone,old karst cave gold deposit,porphyry gold deposit and skarn gold deposit.The form of these gold deposits are related to Tongshi subvoleanie complex.

该区金矿形戚与铜石次火山杂岩体有关,区内金矿类复杂,可划分为隐爆角砾岩带金矿、似层状畏染状灰岩中金矿、古溶洞金矿、斑岩金矿、矽卡岩金矿等5种矿化类

We have sequenced the coding region of Cx26 gene from 190 Taiwanese patients with prelingual deafness and 120 unrelated normal individuals. In the deaf patients, three mutations were found: missense mutation, 551G to A and deletion, 235delC and 299-300delAT.

收集120位听力正常人及190位听障孩童对Cx26基因进行多性比对发现有5种多性,其中有4种多性是之前已被发表的:79G→A(V27I)、109G→A(V37I)、341A→G(E114G)及608T→C(I203T),另发现有一个新的多性(558G→A)。

The researchers used the Lesch typology to classify patients into four types of alcohol dependence: type 1, those who suffer from heay alcohol withdrawal and use alcohol to weaken the withdrawal symptoms; type 2, indicating those who use alcohol for its anxiolytic effects; type 3 ("depression"), coering patients who hae an underlying affectie disorder that accounts for the alcohol abuse; and type 4 ("adaptation"), applied to those who hae premorbid cerebral defects, behaioral disorders, and a high social burden.

研究者使用lesch类学把病人分为四种酒精依赖:1,那些遭受沉重酒精戒断症状并使用酒精减轻戒断症状的人;2,表示那些依赖酒精的镇静效果的人;3,涵盖有可导致酗酒行为的潜在情感障碍的病人;而第4,适用于那些有病前脑缺陷、行为失常或有很高社会负担的人。

The results showed that:(1) Type B of P. shenzhou served as progenitive material is cut and differentiated into type A 79.60%, type B 18.30% and type C 2.20%, and the proportion is stable.

结果如下:(1)深州杨B作为繁殖材料扦插后,深州杨A占79.60%,深州杨B占18.30%,深州杨C占2.20%,且分离比例稳定。

Two different fading procedures were studied. One is the oxidation procedure on polyester fibers,the other is the reduction procedure on wool fibers.The study of oxidation procedure mainly focused on 11 disperse azo dyes and 11 disperse anthraquinone dyes. The study of reduction procedure mainly focused on 7 pyrazolone coupled acid azo dyes and 10 azo disperse dyes which has sulfonic group on the couple component.

本论文研究了11支偶氮分散染料,11支蒽醌分散染料在聚酯纤维上的分子结构与光牢度的定量关系,同时研究了7支以吡唑啉酮为偶合组份的偶氮酸性染料和10支偶合组份上带有磺酸基的偶氮酸性染料在羊毛纤维上的分子结构与光牢度的定量关系。

Other types of titanate are chelate (for greater stability in wet environments), quat (water-soluble systems), coordinate and cycloheteroatom.

其它类的钛酸酯是螯合、水溶、配位和杂环原子

The karyotype of the genus Lycoris has great variation,we find some new karyotypes and new chromosome number by meticulous cytological analysis, especially in Lycoris radiata which karyotypes exist tremendous variation even in different population.

石蒜属植物的染色体核变异很大,通过细致的核分析,发现了许多新的核和染色体数目;其中石蒜在不同居群中的核存在巨大的变异。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。