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Objective In order to understand the role of IL-6 as a neurotrophic factor owing to increasing of nerve fibers in anterior pituitary after adrenalectomy.

目的 了解肾上腺切除后垂体前叶内神经纤维增多的成因及与神经营养因素的关系。

Methods: fifteen sd rats were randomly divided into five groups (n=3), and adrenalectomy was performed and anterior pituitary specimens were obtained after a week. anti gap 43 antibody and anti adrenocorticotrophic hormone, thyroid stimulating hormone, luteinizing hormone, prolactin and growth hormone antibodies were used in the double immunohistochemical staining.

将成年雄性sd大鼠15只随机分为5组(n=3),手术切除大鼠双侧肾上腺,1 wk后取垂体前叶标本分别进行抗gap 43与抗促肾上腺皮质激素、促甲状腺激素、黄体生成素、催乳素、生长激素抗体的免疫组织化学双标染色,观察gap 43 li神经纤维与各腺细胞之间的关系。

The pituitary gland consisted of neurohypophysis and adenohypophysis. The adenohypophysis was divided into anterior lobe, intermediate lobe and posterior lobe.

黄颡鱼的脑垂体由垂体神经部和垂体腺部组成,垂体腺部又由前叶、间叶和后叶三部分构成。

The results showed: 1 All GH deficient children presenting with polyuria and polydipsia had severe ADH deficiency; 2 In 11 GH deficient children without polyuria and polydipsia, 7 were found endogenous ADH insufficient; 3 The children with sho...

结果表明:(1)GH缺乏、伴多饮多尿症状的生长落后儿童均为ADH严重缺乏;(2)GH缺乏、无多次多尿症状的生长落后儿童11例中7例为潜在性ADH缺乏;(3)GH正常、伴多饮多尿症状的生长落后儿童都为ADH部分缺乏;(4)GH正常、无多饮多尿症状的生长落后儿童的下丘脑-垂体后叶功能基本正常。

Results Following orchiectomy 7d,the amount of CGRP-immunoreactive nerve fibers in the AP of the rat increased significantly, with the tortuous varicose fibers more densely distributed than that of NOR and the amount of varicosities increased significantly, while the thick fibers were more thicker than that of NOR,and many thin fibers were sent out from the thick fibers, and the varicosities were more larger.

结果 睾丸摘除7d后,大鼠垂体前叶CGRP免疫反应神经纤维发生了显著变化,表现为膨体型纤维较正常组稠密,分支增多,迂曲成片,盘绕成网,分布更加广泛,膨体数量显著增多;粗纤维较正常组粗,且发出许多迂曲盘绕的细纤维,膨体形态变大。t检验显示,OX组与NOR组垂体前叶内CGRP免疫反应神经纤维的平均面积密度差异显著,OX+V与NOR组差异显著。

When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change.

外源腹腔注射10 IU/g IFN-γ后,同A组相比:D组TNF-α免疫阳性产物在下丘脑视前内侧核、视上核、视前大细胞核、室周核、室旁核、弓状核显著增高,在视交叉上核显著降低,在其它核团较A组无显著差异,在神经垂体、垂体前叶、卵巢中阳性产物表达均较A组显著降低,在子宫中的表达较A组变化不明显;同B组相比:D组TNF-α免疫阳性产物在弓状核显著增高,在视交叉上核、视前大细胞核、室周核、室旁核、腹内侧核、背内侧核、神经垂体、垂体中间部、卵巢各部阳性物质表达均显著降低,在子宫表达变化不显著;同C组相比:D组下丘脑视前内侧核、视上核、室旁核、弓状核、垂体前叶与中间部均显著增高,在视交叉上核、室周核、神经垂体、卵泡、黄体中均显著降低,子宫中表达变化不明显。

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