块菌

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Inject uses a husband ground acerbity natrium is white on the west loose lump content or powder, for wide chart antibacterial, to a series of change bacterium of positive of orchid family name has the powerful effect that fight bacterium.

注射用夫西地酸钠为白色疏松块状物或粉末，为广谱抗菌药，对一系列革兰氏阳性细菌有强大的抗菌功能。

Measure the inhibition by agar piece method. After puddling treatment raise seedlings and dipping root pot culture test, get the growth promoting effect to the strawberry and measure the strawberry leaves and root's PPO activity. so as to get the induced endurance.

采用琼脂块法、拌土育苗和蘸根盆栽生物试验及PPO活性测定法研究供试放线菌对病原菌的皿内拮抗作用、对草莓的促生作用及诱导抗性。

Ultra-fine fibers are made of the use of modern high-tech means a new type of textile materials, with a special equipment, special techniques, special finishing after the fluffy soft, feel comfortable, absorbent, and non-sp is not rotten, anti-bacterial hygiene, and my company Ultrafine Products have unique advantages to address the other issue generated by the equipment hardware, leakage at the end of the grate screw terry fight disease is to produce a bathrobe, shower cap, bath towel, hand make-up kits, facial towel, cleaning gloves, cleaning brush block , clean towel, pumping grid towel, mop cloth, and many were able to rags Jiapin quality.

超细纤维是采用现代高科技手段制成的新型纺织材料，经特殊设备、特殊工艺、特殊整理后蓬松柔软、手感舒适、吸水性强、不霉不烂、抗菌卫生、并且我公司超细产品有独特的优点，解决了其他设备所产生的发硬、漏底、毛圈打拧的箅病，是制作浴衣、浴帽、浴巾、化妆指掌套、面膜巾、清洁手套、清洁擦块、清洁巾、抽格巾、拖把布及多共能擦布的优质佳品。

The odontolith is the hard calcified or calcifying lump which is mainly formed by dental plaque .

牙石是附在牙面上的钙化或正在钙化的牙菌班为基质的团块。

Methods: The model of multi-infarction dementia was made by means of retrograde catheterization infusing embolus, which was made of airdry sterilized blood clot of homogenic rats, to arteriae carotis externa.

取同种大鼠无菌自然干燥血凝块研碎，于颈外动脉处逆行插管注入栓子制作多发性梗死性痴呆动物模型。

The purpose of this study is to screen microorganisms with growth inhibition against plant fungal pathogens. The plate-dilution method was used to isolate the bacteria from salt marshes at Dafeng, Jiangsu. The antifungal activity of the bacterial broth filtrates against 5 phytopathogens was determined using mycelial disk method. The antifungal compositions of four active bacteria were analyzed primarily with chromatography techniques. Two hundred and thirty-five aerobic (92) and anaerobic (143) bacterial strains have been isolated from the coast salt marsh and sixty-five strains, of which 39 are aerobic and 26 are anaerobic showed greater than 30% of inhibition rate against the growth of at least one of the phytopathogenic fungi on petri dish.

为了开发对植物病原真菌生长有抑制作用的药源微生物，本实验采用平板稀释法从江苏大丰盐沼湿地土壤中分离微生物，并通过菌丝块法检测这些微生物代谢产物对5种植物病原真菌生长的抑制活性，进而利用柱层析分析其活性成分；结果共分离得到235株细菌，其中好氧细菌92株，厌氧细菌143株；体外活性测试结果显示有65株至少对1种测试植物病原真菌的抑制率在30%以上，其中好氧菌39株，厌氧菌26株。

The effects of nutrition condition on the activation of stratal microflora, especially the beneficial bacteria including hydrocarbon degrading bacteria, methaneproducing bacteria and nitrate reducing bacteria for oil recovery, were investigated on the basis of the analysis of the stratal microflora in S12 block in Shengli Oilfield.

在考察了胜利油田 S12块注入水和油井产出水中细菌群落状况的基础上，针对烃类降解菌、产甲烷菌、硝酸盐还原菌等有益于采油的细菌群落，研究了营养条件对细菌群落激活的影响。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！