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Objective To compare on affection between cryotherapy and immunosuppressive agent for alloˉgraft of rat sciatic nerve.

目的 探讨应用冷冻法与免疫抑制剂法对同种异体鼠坐骨神经再生效果的比较。

Study on effect of BuqiTongluo Formula on the axon regeneration of sciatic nerve in rats.

补气通络方对大鼠坐骨神经轴突再生的影响。

In DM group, decreasing of density of axoplasm, deposition of fat droplets, swelling and vacuolization of mitochondria and separation or disintegration in lamellar of myelin sheath of the sciatic nerve fiber could be observed, which could be partially improved by EA and KBK.

电镜形态学分析见造模两个月的糖尿病大鼠坐骨神经髓鞘板层有分离现象,线粒体嵴紊乱,有的线粒体嵴完全消失形成空泡线粒体,轴浆内有大量脂肪颗粒沉积;经电针和渴痹康治疗后能减轻这些病理改变。4。无论是电针治疗还是渴痹康治疗均无明显降血糖作用。

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP (hydrogen-peroxide oxidoreductase)逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数(sciatic nerve function index,SFI)测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况,探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用,为外周神经损伤的治疗提供新的理论基础。

we exposed the hibateral sciatic nerves of the rats by means ofexperimental modes,one side was being retained for sixty seconds,the other was justbeing exposed for same time to be the nomal contrl,so we established the rats\'ischiadic nerve crushing injuried models.

我们用实验的方法将大鼠的双侧的坐骨神经进行暴露,一侧钳夹持续一分钟,另一侧作为正常对照组,从而建立了大鼠坐骨神经挤压损伤的动物模型。

Chitosan and pigskin were added into the PLGA conduit of film containing gelatin microsphere (containing bFGF, then this material was planted into rabbit's ischiadic nerve. The experiments indicate that the material has a good biocompatibility, no inflammatory reaction, and no allergic response.

将成功构建的含bFGF明胶微球与PLGA材料加入壳聚糖、粉碎的脱细胞猪皮等改善其机械性能制作成复合导管之后,将此导管植入兔坐骨神经进行相关的观察,初步实验显示此材料在兔坐骨神经部位生物相容性良好,无炎症反应,无过敏反应。

Schwann cells from wallerian degenerating segments of rat's facial nerves were isolated and cultivated. Their morphological, in vitro growth features and typical functional phenotype--〓 were observed.

一、对分离培养的大鼠沃勒变性段面神经雪旺氏细胞与大鼠沃勒变性段坐骨神经雪旺氏细胞及新生乳鼠面神经和坐骨神经来源的雪旺氏细胞的形态、生长等生物学特点进行了比较实验研究。

Before transecting inject 1ml Sodium Chloride in the epineurium. Annular tensile the proximal of epineurium with microscopic forceps annular shear the epineurium 7mm before broken ends with microscopic shear and stretch the epineurium until the two ends distance is 5mm.Use 8-0 suture line to bridge the nerve gap and make a 5 mm nerve tube at the proximal nerve end. Interrupted suture the epineurium tube and the distal nerve end make a 5 mm nerve mamuro for the injury nerve.Results: Postperative observation lasted for 6 weeks regeneration nerve through the 5 mm nerve mamuro.

在大鼠的坐骨神经距股方肌下缘15mm处,用1ml注射器向神经外膜下注入1ml生理盐水,然后于此处切断坐骨神经,用显微镊环形拉伸近端神经外膜,在距断端约7mm处用显微剪环形剪开神经外膜,并继续拉伸外膜使外膜两断端间距约5mm停止,用8-0的无损伤缝线桥接神经外膜两端,在神经近端形成约5mm的神经外膜管,将神经外膜管与神经远端间断缝合,使断伤的神经束间留有5mm的小间室。

Materials and Methods: A 47-year-old woman complained of a right posterior thigh mass that she had discovered two months earlier. Serial examinations showed a large neurogenic tumor about 4.8 cm in maximal diameter originating from the right sciatic nerve. Intraoperatively, we found the tumor encapsulated by the stretching nerve fibers. It was feasible to peel off these fibers from the tumor; therefore, we removed the tumor without severing the sciatic nerve.

材料及方法:一个四十七岁的妇女,最近两个多月抱怨右大腿后侧有一肿块,相关的检查疑似为神经源起之肿瘤,最大直径为4.8公分,术中发现肿瘤本身被撑开的坐骨神经束包覆住,而肿瘤可在小心剥开这些神经束后被移除掉,坐骨神经可被完全的保留而不需要牺牲。

The rabbits were immobilized according to the standard of X_knife before CT scanning. The interrelation of the silica gel tube and bony structures was analyzed, a nerve segment was selected, which could be localized according to the related bony structures as irradiation target. A radiosurgery treatment planning was performed on the selected nerve segment, its changes of pathology and ultrastructure were observed. Results: Base on the dissection, the rabbits' sciatic nerve was composed of the seventh lumbar nerve and first and second sacral nerves.

通过解剖分析兔坐骨神经主干的解剖位置和行径,选择照射靶点,放置硅胶管进行标记,按X_刀标准行CT扫描,从CT图像上分析硅胶管与荐骨和髂骨的位置关系,确定可以用骨性结构定位的神经段作为照射靶点,利用立体定向技术单次大剂量照射坐骨神经,观察其病理及超微结构变化。

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