坐骨神经的

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Physiological oscilloscope was used to examine the latent period on both sides of rat sciatic nerve and amplitude of evoked potential.

同时用生理示波器测定大鼠两侧坐骨神经的潜伏期和诱发电位的波幅。

Conduits absorption and surrounding tissue cicatrization were observed at 1、2、4 weeks after neurotomy. And the nerve regeneration and functional recovery were evaluated by electrophysiology, histological changes, morphometric analysis, and weighing of gastrocnemius muscles at 6, 8 weeks afterneurotomy.

分别于术后1、2、4周观察桥接鞘管周围的瘢痕形成及吸收情况，并于术后6、8周行神经电生理、组织学观察、图象分析、腓肠肌湿重检测，比较各组大鼠坐骨神经的再生与功能恢复情况。

Morever, it could prevent the changes of never pathology.

该药对血糖及坐骨神经的形态学也有一定程度的影响。

The main goal of this subproject is to build a transverse biomechanical model of sciatic nerve of Wistar rat by in vivo experiments.

中文摘要本子计画的主要目标是透过活体实验，建立 Wistar 白鼠坐骨神经的横切面生物力学模型。

130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.

实验方法：将130只Wistar大鼠随机分为五组：正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组，除正常组外，其余各组手术切取坐骨神经4mm后，做坐骨神经硅胶管桥接术，造成周围神经损伤模型，取患侧足三里、三阴交、环跳穴，术后第四天开始电针，每两天一次，每次10分钟，治疗10周后处死动物，取出硅胶管内新生的坐骨神经，纵切片，HE染色、S100免疫组化染色、Sevier-Munger银染，观察坐骨神经纤维再生情况，以健侧同段坐骨神经做对照；取患侧脖肠肌称取湿重，HE染色，观察腓肠肌肌细胞直径，生化测量腓肠肌肌糖元、乳酸。

Three animals randomly selected from three groups were killed on Day 0 to determine the baseline of PLA2 activity within unmanipulated rat sciatic nerve; other 39 animals underwent operation for model-making of acute sciatic （Maves-modified Bennet-Xie model） on right sciatic nerves, while Sham operations were performed on left sciatic nerves.

第0天，每组随机抽取1只共3只大鼠处死，测量双侧坐骨神经节段磷脂酶A2活性为基线，其余39只大鼠右侧坐骨神经制作急性坐骨神经痛模型（Maves改良的Bennet-Xie模型），对侧行Sham手术；第7天，每组随机抽取3只处死，测量治疗前模型和Sham坐骨神经节段的磷脂酶A2的活性；第14天，剩余大鼠处死后测量各组模型和Sham坐骨神经节段的磷脂酶A2的活性。

METHODS:①Sixty samples were randomly divided into two groups(test and contrast groups,n=30 respectively),and the sciatic nerves of the toad was isolated.Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

Over an eight-year period, Pavarotti had cancelled 26 out of 41 scheduled appearances at the Lyric and the decisive move by Krainik to ban him for life was well-noted throughout the opera world, after the performer walked away from a season premiere less than two weeks before rehearsals began, saying pain from a sciatic nerve required two months of treatment.

因为在大约八年的时间内，帕瓦罗蒂取消了26场演出，而预定的演出仅有41场。卡拉尼克因此决定他终身不得再在芝加哥歌剧院演出。在一次季度公演中，离预演不到两个星期的时侯，帕瓦罗蒂离开了，声称他需要两个月来治疗坐骨神经的疼痛。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。