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The study of the histopathology showed that the haemolymph in the disease crab decreased sharply, and was replaced by large amount of parasites, which was observed in muscle, heart and hepatopancreas from the disease crab. The pathological characteristics mainly appeared as cells swelling, necrosis, nucleus condensation and collaspe.

组织病理学观察发现,病蟹血淋巴急剧下降,代之以大量的寄生原虫,该寄生虫在病蟹的肝胰腺、鳃、心脏、肌肉等部位大量侵袭,并引起这些组织发生以坏死为主的变质性病变,其病理特征主要表现为:细胞肿胀、变性、坏死,某些细胞的细胞核固缩、碎裂或崩解。

The results the fresh fracture of meck of femur(≥20year old)treatrd with internal fixatioon with compressor screw,≥150°argulation,could obviously decrease the curvation and shearing stress of fracture line.

本文资料说明,新鲜的≥20岁的股骨颈骨折采用本法治疗,可显著减少股骨颈骨折线的弯矩和剪应力,故可保护骨折线中新生血管,避免并发股骨头坏死,有较好的临床应用价值,尤其是减少并发股骨头坏死方面。

The pathologic changes were observed by light and electron microscope.Results The liver tissues of the rats in model group showed obvious hydropic and fatty degeneration.And the necrosis foci of hepatocytes were seen in liver tissue.

结果 模型组大鼠的肝组织呈现明显的水样变性和脂肪变性,并可见肝细胞坏死灶,姜黄素治疗组的肝细胞未见坏死改变,水样变性和脂肪变性程度减轻,以高剂量姜黄素组的治疗效果最为明显。

Results The liver tissues of the rats in model group showed obvious hydropic and fatty degeneration.And the necrosis foci of hepatocytes were seen in liver tissue.Hepatocytes in curcumine treatment group showed no necrotic changes,and the degree of hydropic and fatty degeneration was markedly reduced,especially in the high-dose curcumine treatment group.

结果 模型组大鼠的肝组织呈现明显的水样变性和脂肪变性,并可见肝细胞坏死灶,姜黄素治疗组的肝细胞未见坏死改变,水样变性和脂肪变性程度减轻,以高剂量姜黄素组的治疗效果最为明显。

One hundred and twenty-one cases comprised of absecesses, secondary peritonitis, necrotizing fascitis and wounds with devitalized were studied.

应用标准的微生物方法检测了脓肿、继发性腹膜炎、坏死性筋膜炎和坏死性组织创口4类外科脓液标本的微生物分布,并同时进行需氧菌和厌氧菌的检测。

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性,建立了一种可同时检测6种对虾病毒的RT-PCR方法,并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响,本研究应用体外扩增的新生大鼠中脑NSCs,使用免疫组织化学技术,观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中,雏鹅临床表现为高发病率和高死亡率,食欲和饮水减少,拉白色、黄绿色稀粪,伴有神经症状;常规组织学观察,脾脏和胰腺肿大,有白色坏死斑点,肠道出血和坏死,肝脏肿大变性,肾脏肿大变性有尿酸盐沉着等;用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究,结果表明:胰腺中发现病毒包涵体,肠道中大量纤维素以及胶原纤维增生,肾脏、肺脏大量含铁血黄素沉着,免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少;电镜下多种细胞呈现线粒体水肿,多种实质细胞的染色质固缩,染色质边集,特别是细胞内容物如脂肪滴增多的病理变化特征。

Steroid-induced avascular necrosis of femoral headwas a refractory disease,it was caused by the lack of local bone tissue repair and regeneration,and lead to collapse of femoral head which resulted in hip joint dysfunction.

激素性股骨头坏死是世界性的难治病,其主要受限于局部坏死骨组织修复作用的不足,最终造成股骨头塌陷而导致肢体的功能障碍。

PartⅡExperimental Study on Acute Hypatocyte Necrosis and Apoptosis with MRI, DWI and MRS Objective: To demonstrate the ability and value of MR imaging, diffusion-weighted imaging, and MR spectroscopy in detecting and characterizing necrosis and apoptosis in vivo with a comparison to the pathology after establishment of hepatic necrosis and apoptosis animal model by different surgical ligations of hepatic blood supply vessels.

目的:通过手术结扎的方法建立大鼠肝细胞坏死和凋亡的动物模型,将不同时间点的MRI、DWI和MRS检测结果与病理学所见进行对照研究,以评价MRI、DWI及MRS在检测及鉴别肝细胞凋亡和坏死两种病理改变的能力与价值。

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