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The region between the circular brown band generated by cell lipid exosmosis and the band of the wound in the non-transgenic cotton Z35's leaves, is wider than that of the transgenic cotton T-34's leaves, and no micro-HR was found in Z35, the intensity of leaves wilting is much serious, by contrast, micro BR was found around the wound region within the transgenic cotton leaves inoculated with the pathogen, but the micro-HR was not found in the transgenic cotton treated with water and around wound region of non-transgenic cotton Z35 leaves which showed that there was micro-HR defence in transgenic hpa1xoo cotton.

棉花黄萎病菌侵染叶片后,非转基因棉花叶部伤口周围细胞无微过敏性反应,受病原菌侵染的叶片萎蔫程度较重;转hpa1xoo基因棉花叶部伤口周围细胞有微过敏反应,叶部刺伤处仅有较小的坏死斑,且不相连,发病较轻,而清水处理的转基因棉花与非转基因棉花叶片中伤口周围均无微过敏反应,说明病原菌侵染诱导转hpa1xoo基因棉花产生微过敏防御反应,转hpa1xoo基因棉花较非转基因棉花有较强的抗病性。

In contract, the tumor tissue of control group kept morphology, tumor nodules composed of undifferentiated carcinoma, and some karyokinesis could be seen, some spotty necrosis were found occasionally in where the structure among tumor cells were loose associated very slight inflammatory response; At the 21 days after injected AdCMVIL-12, the tumor boundary of AdCMVIL-12-Ⅰ group were clear and felt hard by touch, section showed grey.

同期的对照组绝大多数肿瘤细胞形态完好,排列紧密,由大量未分化的肿瘤细胞组成并可见多个核分裂像,偶尔可见散在的点状坏死灶,瘤细胞之间结构松散,炎症反应很轻;AdCMVIL-12注射后第21天时,AdCMVIL-12-Ⅰ组的肿瘤组织经手摸感觉瘤体境界清楚、发硬,切面呈灰白色。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论:(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞,(2)与LAK相比,用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长;CD〓AK是一类值得开发的抗瘤效应细胞;(3)CD〓AK能够介导MHC非限制性细胞毒活性,可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞;(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡,两者并存于同一体系,推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

Results:pedunculated omentum majus-cirded trachea transplantation ensured an early re-establishment of blood circulation of transplanted trachea and maintenance of its normal animate states; allogeneoilc transplantation of trachea activated bodys immune mechanism. resulting in a chronic process of rejection and finally leading to progressive necrosis of transplanted trachea tissues: chondroregeneration occurred at every period under perichondrium after transplantation. and the degree of regeneration was related with favorable blood circulation; the emplogyment of a supporting tube in trachea,under the condition of favorable re-establishment of early blood circulation, could preventgranulation tissue hyperplasia and keep trachea cavity unobstructed.Postgraduate Ding XiaoQuan Directed by-Lin LeShen

结果 带蒂大网膜包绕移植段气管行腹腔移植,早期重建了移植段气管的血液循环,保证了移植气管的早期成活及维持其正常生机状态;同种异体气管移植所激发机体的免疫机制,引起一个慢性排斥反应过程,导致移植段气管组织进行性坏死;软骨再生存在于移植后各个时间段,其再生程度与良好的血液循环有关;气管内支撑管的应用可防止肉芽组织增生堵塞管腔,从而保证气管管腔通畅。

Methods: A brain concussion was produced in rats using a simple pendulum device model. One control (n=12) and six experimental groups were used(n=12 in each group). The cerebral concussion groups were assigned to sacrifice times ranging from 1 day to 24days after injury.A Morris Water Maze memory paradigm was used to assess learning and memory function. Pycnosis degeneration or necrosis neurons in the locus cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation were observed and counted after brain concussion. Cholinergic neurons in the basal forebrain and brainstem reticular formation were identified and quantitated using choline acetyltransferase.

应用自制单摆式闭合性机械打击装置建立脑震荡大鼠模型;成年SD大鼠随机分为1个对照组(n=12)和6个脑震荡组(即伤后1、2、4、8、16、24d,n=12);应用Morris水迷宫(Morris Water Maze,MWM)实验评价大鼠的学习记忆功能;用光镜对大鼠的上述脑区的固缩变性坏死细胞进行观察和计数;用免疫组化方法检测基底前脑和脑干网状结构的胆碱乙酰转移酶(cholin acetyltransferase,ChAT)阳性细胞的表达。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后,血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少,Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少,血小板聚集程度及红细胞聚集指数显著上升,病理切片显示胸主动脉内皮细胞破坏明显,脑心肾组织内的血管进一步增厚甚至几乎阻塞,导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR,脑体重比显著升高;心肌内出现明显的缺血梗塞疤痕灶,心体重比显著升高;肾小球明显萎缩、玻璃样变性,肾小管也变性甚至坏死,使肾硬化程度显著增加呈颗粒性固缩肾,肾体重比也显著减少。

Erythematous plaques with violaceous and edematous patchesin the palms, soles, and other high-pressure areas are usuallymild and resolve in a week or 2. The problem may, however, evolve into blistering desquamation, crusting, ulceration, and epidermal necrosis if the next chemotherapy cycle is not delayed or thedose reduced. The reviewers point out that palmar-plantar erythrodysesthesia is often uncomfortable and can interfere with the abilityto carry out normal activities.

在手掌、脚跟和其它高压区域的红肿斑块,有紫色以及水肿的片状病灶,通常是轻微且会在1~2周内缓解;然而,若下一次的化学治疗疗程没有延后、或是降低剂量,这个问题可能会恶化成水泡、结痂、溃疡与表皮坏死;作者指出,手掌脚掌红肿感觉异常通常是不舒服的、且可能影响日常生活。

Therefore, the purpose in this study is to observe the change of blood vessel endothelium function and cytokine (tumor necroses factor-α TNF-α, interleukin-6 IL-6) and to investigate the mechanism of Carvedilol improve cardiac function in the patients with CHF.

本实验通过观察CHF患者血管内皮功能和细胞因子:肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)的改变,以及使用卡维地洛后CHF患者这两方面的变化与心功能的改善的相关性,并探讨卡维地洛对CHF改善心功能的可能作用机制,为卡维地洛治疗慢性充血性心力衰竭提供临床依据。

After bleeding sloped, the hemoglobin amount in films or gauzes were measured to determine the hemostatic effect Using a randomized design, one incision in each animal was treated with C-CM membrane, the influence of C-CM film on the growth of bacteria (Staphylococcus aureus ATCC 27853. Escherichia coli ATCC 25922. Monil lia albicans ATCC 14053)were observed in vitro with solid medium. Rusults: It was observed that C-CM membrane had no toxic effects on surrounding tissue and was slowly degraded after implanted into rat leg muscular porch ; The results of hemostatic experiment showed that C-CM film adhered well to incisions.

结果:C-CM膜植入动物肌肉中,周围组织无明显炎症,未发现组织变性和坏死,可在组织内缓慢降解,具有良好的组织相容性,6周后反应接近外科缝合丝线组;止血实验结果发现:C-CM膜组和纱布组的出血时间分别为83.5±9.333s和117±15.9275s,血红蛋白光度吸收值为0.9204±0.7559和1.9764±0.7205,二组比较差异显著(P<0.05),说明C-CM膜有一定的止血作用;促进创面愈合实验结果显示:不同时间段C-CM膜组肉芽组织较对照组厚,其中胶原纤维含量亦较多,C-CM膜组的创面愈合时间早于空白对照组,伤口愈合较快;抑菌实验显示C-CM膜对三种菌产生接触性抑制。

The highest relative defense effect on different diseases were analyzed: 88.61 percent (NO.5 at 50ppm) against powdery mildew, 77.63%(NO.5 at SOppm) against head blight. Although the RDE against stripe rust is lower than the formers, NO.5 at SOppm can induce obvious HR on wheat leaves.

对小麦白粉病的防治效果最高可达86.61%(5号100ppm);对小麦赤霉病的防治效果最高也有77.63%(10号100ppm);并发现50ppm的5号提取物处理后能使感染条锈菌的小麦产生明显的过敏性坏死反应。

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