均质的

In order to study the therapeutic effect of Sodium Hyaluronate injection of articular cavity and taking orally "JIN GU TONG XIAO WAN"(JGTXW, a TCM preparation for osteopathy disease) in the treatment of osteoarthritis of knee, 130 cases of OA were randomly divided into the treated group (66 cases, treated by SH and JGTXW) and control groups(64 cases, treated only by SH); The effect such as local pain, tumidness, joint movement and so on of the two groups in one year were observed and evaluated according to Lysholm criterion.

骨性关节炎是一种常见的老年退行性关节软骨病，随着我国人口的老年化增高，发病亦越来越多，严重影响老年人的生活质量，对膝关节骨性关节炎的治疗方法多，但疗效都均不太理想，不能阻止或延缓疾病的进展，近年来，以透明质酸制剂为代表的氨基糖类黏弹性物质补充疗法，在防治OA的基础研究和临床应用上取得了良好效果，为OA治疗寻找到了新的途径〔1〕。

At present,the rate laws and reaction mechanisms of pyrite oxidation,pyrite and chalcopyrite formation,uraninite and magnetite dissolution in aqueous solutions are mainly studied,and it is discovered that (1)oxidation rate of pyrite controlled by surface reaction assume fractional dependence on Fe3+ and O2 molalities in acid solutions;(2)Pyrite cannot nucleate directly from solutions below 300 ℃,it can only form initially through sulfidation of a FeS precursor. The rate equation of reaction between FeS and H2S to form pyrite is of the second order;(3)Chalcopyrite is formed by reactions of pyrrhotite or pyrite with Cu2+,the former proceeds via a series of mediate metastable phases of Cu-Fe sulfide,whereas the rate equation of the latter is apparently of the first order which is controlled by surface reaction;(4)The nonlinear behavior of magnetite dissolution at acid pH can be represented by a shrinking core model coupling with surface reaction and diffusion transport.

目前主要研究了水溶液中黄铁矿氧化、黄铁矿和黄铜矿形成、晶质铀矿和磁铁矿溶解的速率定律和反应机理，发现：(1)酸性溶液中黄铁矿的氧化速率对Fe3+和O2浓度呈分数依赖并受表面反应的控制；(2)低于300 ℃时黄铁矿不能从溶液中直接成核，而需初始地通过FeS先驱物的硫化生成，FeS与H2S反应形成黄铁矿的速率方程为二级；(3)磁黄铁矿或黄铁矿与Cu2+反应均可形成黄铜矿，前者经历了一系列准稳的Cu-Fe硫化物的中间物，后者的速率方程为表观一级并受表面反应的控制；(4)酸性pH时磁铁矿的非线性溶解行为可采用表面反应扩散输运耦合的收缩核模型来描述。

In this study, EPMA and LA-ICP-MS were used to determine the major and trace element concentrations, respectively, of apatites from Transhimalayan granitoids. The results indicate that F, Mn, Sr and REEs in apatites generally show good correlations with compositions of their host magmas and thus have high potential to be utilized as petrogenetic tracers. More specifically, F and Mn contents in apatites are covariant with the aluminosity of the host rocks so that can be used as an indicator for magma differentiation. Combining with Sr and REE data, which show significant variations in apatites from different rock types, these elements may be furthermore used to construct "discrimination diagrams" for more detailed investigations of complex petrogenetic processes such as magma mixing and compositional heterogeneity.

本研究中，电子微探以及雷射烧蚀感应耦合电浆质谱术分别被用以量测藏南火成岩中磷灰石的主量与微量元素含量，微区分析的结果显示，磷灰石的氟、锰、锶以及稀土元素均与岩浆源成分展现良好的对比关系，揭示了它们应用在岩石成因示踪上的优秀潜力：其中，磷灰石氟、锰含量的变化与原岩的铝饱和情形息息相关，因此可以作为岩浆分异程度的指标；而锶以及稀土元素则在不同岩石类型来源的磷灰石中展现了明显的丰度变化，因此综合这些元素将有机会进一步建立岩浆来源的「鉴别图」，甚而以此讨论岩石成因，例如：岩浆混合与源区组成的不均一性。

Objective To study the relationship of endangium proliferation and the expression of metalloproteinases and tissue inhibitors of metalloproteinases after angioplasty of iliac artery in rabbits to investigate the probable mechanism of composite Danshen pill in prevention and treatment of restenosisMethods 30 white male rabbits of Japan （the average body weight was 25～30 kg）were randomly divided into 3 groups:normal control group,model group (intima destroyed by balloon and given hypercholesterol diet),CDP group (intima destroyed by balloon and given hypercholesterol diet plus drug CDP 150 mg/d),10 ones each groupResults The results of iliac artery angiogram and the analysis of pathology:there were lumens stenosis,thinner intima,smaller intima area,in CDP group compared with those in model group,and ratio of intima and tunica media thickness and area among each group (P＜001)Immunohistochemistry analysis:the MMP2,MMP9,TIMP1,TIMP2 in model group significantly increased than those in control group (P＜001)The CDP group had lower MMP2 and MMP9 expression,higher TIMP1 and TIMP2 expression,and increased MMP2/TIMP1,MMP9/TIMP2 than model group (P＜001)The thickening of vascular neointima and stenosis degree of vascular lumen were relevant to MMP2 and MMP9 (r=0896,P＜001)Conclusions MMP and TIMP play the very important role in the restenosis process of arteryCDP could inhibit the thickness of vascular neointima after balloon injury,the probable mechanism of which may be inhibiting collagen formation,smooth muscle immigration and decreasing hyperplasia of intima by interfering expression of MMPs and TIMPs

目的 研究兔髂动脉成形术后血管内膜增生和基质金属蛋白酶及其抑制物表达之间的关系，探讨复方丹参滴丸预防再狭窄的可能机制。方法健康成年二级雄性日本大耳白兔30只，平均体重25～30 kg。随机分为3组：正常对照组10只，模型组10只（球囊内膜剥脱加高胆固醇饮食），治疗组10只（球囊内膜剥脱加高胆固醇饮食以及复方丹参滴丸150 mg/d）。结果兔髂动脉造影、血管病理图像分析检测结果：①复方丹参滴丸组较模型组血管造影示管腔直径狭窄、内膜厚度减少、内膜面积减少、内膜厚度和中膜厚度比、面积比，各组之间有显著性差异(P＜001)。②免疫组化分析：模型组MMP2、MMP9、TIMP1、TIMP2表达均高于对照组（P＜001）；复方丹参滴丸组MMP2、MMP9的表达低于模型组，而TIMP1、TIMP2的表达高于模型组；TIMP1/MMP2、TIMP2/MMP9明显增加，差异有显著性（P＜001）。③内膜的增生以及管腔的狭窄程度与MMP2、MMP9有很好的相关性(r=0896，P＜001)。结论 MMP和TIMP在再狭窄形成中起重要作用；复方丹参滴丸能够明显抑制血管损伤后的内膜增生，可能的机制是通过影响金属蛋白酶MMP2、MMP9及其抑制物TIMP1、TIMP2的表达从而抑制胶原的生成、平滑肌的迁移、增生，而减少内膜的增生。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

Associated minerals are primary glauberite, halite and terrigenous mud; while calcite is absent or rare. The X-ray diffraction analysis shows that the mud-sized crystalline dolostone has an average CaCO3 mole content of 57.30% and an average degree of order of 0.40, revealing the characteristics of higher calcium content and lower degree of order. It is closely intergrown with glauberite, indicating that the aqueous medium in the depositional environment of mud-sized crystallinedolostone was hypersaline water or dilute brine. The temperature of water in the depositional environment calculated according to the oxygen isotope data averaged 30.5℃ and the minimum homogenization temperature of inclusions in primary glauberite crystals associated with mud-sized crystalline dolomite was 44℃. The two groups of temperature data are close, indicating that the temperature of the water in the paleolake environment during the deposition of mud-sized crystalline dolostone ranged from 30.5℃ to 44℃.

泥晶白云石在阴极发光下呈红色、橙红色，与其伴生的矿物除原生钙芒硝、石盐、陆源泥质外，方解石没有或极少；X衍射分析结果表明，泥晶白云石的CaCO3摩尔含量平均为57.30%，有序度平均为0.40，具高富钙、低有序的特征；与钙芒硝岩的紧密共生，说明泥晶白云岩沉积环境的水介质为高盐度的咸水或淡卤水；根据氧同位素数据计算的沉积环境的水体温度平均为30.5℃，与泥晶白云石伴生的原生钙芒硝晶体中的包裹体均－温度最低值为44℃，这两组温度数据基本接近，表明泥晶白云石沉淀时环境水体的温度大致在30.5-44℃之间。

The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and OH initiated hydroxylation of salicylate, and inhibit O2(superscript -) mediated light chemical reduction of nitroblue tetrazolium effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.

牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用，降低DPPH的吸光值、抑制O2介导的NBT光化学还原及OH作用下的水杨酸羟基化作用，其中牡丹花瓣提取液清除DPPH自由基的活性高于200μg/mL BHT，但低于80μg/mL Vc.6个品种的牡丹花瓣提取液，在4种测定系统中均具有一定程度的抗氧化能力，且水提取液的抗氧化活性略高于体积分数50%乙醇提取液，深色花瓣提取液对不同自由基的清除能力较强。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

These results suggest that the diagenesis material of the Xiangshan volcanic intrusive complex was derived from crustal sources. The sources either had relatively lower maturity or had more juvenile mantle-derived igneous rocks than the exposed basement metamorphic rocks. Involvement of a small amount mantle-derived magma during the formation of the Xiangshan volcanic-intrusive complex was also possible. The diorite enclave, sub-quartzmonzonitic porphyry and lamprophyre were probably different mixing products between mantle and crust materals.

上述同位素示踪结果充分证实：相山火山-侵入杂岩的成岩物质主要为壳源，但与该区出露的基底变质岩相比，其物源区的成熟度相对较低或含有一定量的火成物质，并且不排除在其成岩过程中少量地幔物质的介入；闪长质包体、次石英二长斑岩及煌斑岩的成岩物质均含有一定量的地幔组分，可能为壳-幔混合作用的产物，只是受地壳物质混染的程度不同而已。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。