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PAL activity, chitinase activity, total Polyphenol content and total Flavonoids content in soybean leaves induced by the same crude toxin and race were similar, it stated that the crude toxin is an important factor which can induce resistant substances, but the induced speed and the induced intensity by the crude toxin and conidium were different. Firstly, the induced speed of crude toxin which induced PAL activity and total Flavonoids content of resistant soybean varieties was faster than speed of C.

同一生理小种的粗毒素与灰斑病菌对大豆叶片内PAL活性、总多酚类含量、总黄酮含量以及几丁质酶活性等几种生化指标均表现出相似的诱导作用,由此可推断粗毒素是诱导大豆叶片内的PAL活性、总多酚含量、总黄酮含量以及几丁质酶活性产生变化的主要生物因子,只是二者在对抗性不同的大豆品种的诱导速度及强度上存在一定的差异。

The absorption of root system was the main sources of nitrogen in the plant.The effects of matrix ventilation on the abaorption,transportion and metabolism of nitrogen in the root system of cucmber were discussed deeply.The results showed that contents of ammonium,nitrate,free amino acids and soluble protein in cucumber roots were higher in G1 treatment.Contents of ammonium,nitrate and free amino acids in cucumber roots were the trend of the dropping after the first rising in F1 treatment,but contents of soluble protein was lower11.03%than that of the control.

根系吸收是植物体内氮素的主要来源,本研究深入探讨了基质通气状况对黄瓜根系氮素吸收、运输和氮代谢的影响,结果表明G1通气处理根系的铵盐、硝酸盐、游离氨基酸和可溶性蛋白含量均较高;非通气处理(F1处理)根系内铵盐、硝酸盐和游离氨基酸含量呈先升高后降低的趋势,而可溶性蛋白含量始终较低,比对照降低11.03%。G1处理根系硝酸还原酶、亚硝酸还原酶、谷草转氨酶、谷丙转氨酶、谷氨酰胺合成酶活性均较高;而F1处理的酶活性在处理末期显著降低,其中谷氨酰胺合成酶活性比对照降低33.33%。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏,匀浆、研磨,经高速离心,超速离心和蔗糖密度梯度离心,分离肝脏质膜,得到肝脏质膜的电镜切片:其次,用去垢剂TritonX-100增溶膜蛋白,利用放射受体分析法,测定细胞膜及膜蛋白与~(125)I-hGH的结合活性,均得到理想的饱和曲线;再次,将两点结合试验法与凝胶层析法结合检测到特异结合物,然后,经SDS-PAGE电泳分析,纯化后的样品呈现一条带,与标准分子量比较,得出分子量为119KD。

The regulatory effect of corticosteroids treatment on growth factors is mainly at early stage of tendon healing, but hyaluronic acid works at early and late stages. Corticosteroids always facilitate the expression of the growth factor, whereas hyaluronic acid not only promote but also inhibit the expression of growth factor.

皮质激素在肩袖损伤修复过程中对4种因子的调控主要在早期;透明质酸在修复早期、后期均有不同程度的作用;皮质激素的调控作用主要是促进生长因子的表达,而透明质酸存在双向调控作用。

Simulated regression analysis showed that the exponential increase of MDA content, plasmamembrane permeability, auto-oxidation rate of the organ and O2 produc-tion rate and parabolic decrease of SOD activity and GSH content as well as the logarithmic decrease of CAT activity in the stressed leaves were found with the decrease of soil water content....

经回归模拟显示,蔗叶MDA含量、质膜差别透性、O_2产生速率和组织自动氧化速率均随土壤含水量的降低呈指数增加,SOD活性和GSH含量呈抛物线变化,而CAT活性呈对数降低;逐步回归分析定量地指出了水分胁迫下蔗叶SOD和CAT在清除活性氧、防止膜脂过氧化和质膜破坏中的协同作用关系,且受O_2产生的影响;经卡方测验证实,该回归模型各参数与大田干旱胁迫的实际结果相符。

The degradation rates of copolyanhydrides were much slower than those of homopolyester anhydrides, and decreased with the increase of the ratio of CPP.

含Aspirin的均聚酸酐基质片的体外药物释放速率随时间逐渐加快,而共聚酸酐基质片的释放速率慢得多,且随CPP比例增大而降低,可保持一段时间的稳定释放速率。

Qualitative and quantitative analyses were carried out for the analyte under the multiple reaction monitoring mode after the chromatographic separation on a Waters Atlantis T3 column (150 mm×2.1 mm, 3 μm). The quantitation was performed with deuterated N-nitrosodiethanolamine as internal standard. The limit of quantitation for N-nitrosodiethanolamine was 50 μg/kg. The mean recoveries were 89.1%-98.2% at the spiked levels of 50-250 μg/kg, with the intra-day precision less than 9% and the inter-day precision less than 11%.

NDELA经waters Atlantis T3色谱柱(150mm×2.1mm, 3μm)分离后在多反应监测模式下进行串联质谱定性及定量分析,以d8-NDELA为内标定量NDELA的方法定量限为50μg/kg;在50~250μg/kg的3个添加水平范围内的平均回收率为89.1%~98.2%,日内精密度均小于9%,日间精密度均小于11%。

Results SDF-1/CXCR4 was present in the basal epithelial cells of normal conjunctiva. Of 20 pterygia cases, 17 expressed SDF-1/CXCR4 in the full thickness of the epithelium and CXCR4 was also found in the inflamed cells and vascular endothelial cells in the tissues; 3 primary pterygium cases showed the same staining pattern as that in normal conjunctiva.

结果 在正常结膜组织中SDF-1/CXCR4仅表达于基底部上皮细胞;胬肉组织中有17例,全层上皮细胞均有SDF-1/CXCR4的表达,胬肉基质层中浸润的炎性细胞、血管内皮细胞均有CXCR4的蛋白表达;3例初发性翼状胬肉表达方式与正常结膜相似。

The slightly negative δ^30Si values of both thick-bedded and anatase-rich siliceous rocks are similar to those of hydrothermal and biogenic siliceous rocks. The ranges and mean values of δ^18O values are similar.

两种硅质岩的δ^30Si值为变化较小的负值,与热水沉积和某些生物成因硅质岩的硅同位素组成相似,两者的δ^18O值范围和平均值均相似。

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