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We found that the release of ROS in rat astrocytes was significantly increased by the stimulation of calcium ionophore or opsonized zymosan, which are known to trigger respiration burst in phagocytes by NADPH oxidase pathway. Further study indicated that diphenylene iodonium or apocynin , two inhibitors of NADPH oxidase, significantly suppressed ROS release caused by calcium ionophore or opsonized zvmosan.

我们发现在用吞噬细胞呼吸爆发刺激剂钙离子载体或酵母多糖刺激星形神经胶质细胞时,细胞O〓和H〓O〓产量均明显增加,而NADPH氧化酶的抑制剂二联苯碘(diphenylene iodonium,DPI)或夹竹桃麻素(apocynin,APO)均可以明显地降低刺激剂引起的细胞O〓和H〓O〓产量增加。

In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21, The expression of recombinant prolyl endopeptidase in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains.

在高密度发酵条件下,G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重,均明显优于对照菌株PA1和BL21;重组蛋白脯氨醯内肽酶在G830和PA1中获得稳定高表达;重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。

Geochemical study indicates that constant elements, microelements and trace elements in manganese ores and protore wholly take on thermal sedimentary characteristics, the siliceous limestone, silica-bearing limestone and bioclastic limestone is predominant protore beds of manganese-capped ores.

通过对锰矿石和矿胚层岩石中常量元素、微量元素及稀土元素等地球化学的研究,均具有热水沉积的特征,硅质灰岩、含硅质灰岩和生物碎屑灰岩是锰帽型矿石最主要的矿胚层。

Take silica gel chromatography eluting with EtOAc / petroleum ether(1:9~5:5). The production was washed several times by MeOH, and dissolved in dimethyl formamide to recrystallize, we got yellow power crystal.

结果:(1)HPLC检测含量大于95%,符合对照品的要求;(2)理化鉴别实验均与文献吻合;(3)紫外、红外、质谱、核磁共振的光谱结果均与文献值一致,确定产物为金松双黄酮。

Results Different degree expression of FasL can be observed in the trophoblasts of all 40 placentas, but no expression of FasL can be seen in endometrium tissues.

结果 40例胎盘组织中滋养叶细胞均有不同程度的Fasl表达,宫内膜组织的腺体及间质细胞等均未见明显Fasl表达。

According to this scheme, the Longmaxi Formation is only referred to as the black siliceous and carbonaceous shales at the base of the Silurian strata. All the carbonate rocks above it have been redelineated and redivided into new lithostratigraphic units.

新方案除对志留系底的黑色硅质碳质页岩沿用龙马溪组这一岩石地层单位名称外,其上的碳酸盐岩均重新划分和建立了新的岩石地层单位,并确定其涵义和时限标准。

DNA efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 C、D法提纯的质粒DNA的含量、纯度和收获率均较A、B高,上述4 方法提纯的质粒DNA都能进行酶切反应。

Dna efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 c、d法提纯的质粒dna的含量、纯度和收获率均较a、b高,上述4种方法提纯的质粒dna都能进行酶切反应。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

HPLC/MS/ MS was used to analyse the nucleosides in Cordyceps sinensis .and its counterfeits and adulterations. Compared the samples with the standard samples,the result showed that the main fragment chromatogram were similar.The four nucleosides in Cordyceps sinensis .and its counterfeits and adulterations were identified by MS/ MS.The totle content of uridine、hypoxanthosine、guanosine、adenosine in Cordyceps sinensis .was from 2.473 mg/g to2.830 mg/g ,the content of adenosine was from 0.850 mg/g to1.009 mg/g .

应用HPLC-MS对冬虫夏草及其混淆品与伪品中的四个核苷成分进行二级质谱定性分析,并与对照品进行比较,结果显示,冬虫夏草及其混淆品与伪品均能产生与相应对照品一致的主要碎片离子峰,进一步说明,冬虫夏草及其混淆品与伪品中均含有尿苷、肌苷、鸟苷及腺苷四种核苷成分。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。