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Results Pulmonary carcinosarcoma and blastoma both were found to be rare malignant tumors, and both epithelial and mesenchymal elements can be seen under the microscopy with different characteristic features.

结果 肺癌肉瘤及肺母细胞瘤均为肺内罕见的恶性肿瘤,镜下均可见上皮及间质两种成分,但又有其不同之处,手术前很难确诊。

Results The pathological changes in the nuclei mentioned above included the crimple and ruture of nucleus membrane,mitochondria degeneration and other degenerative structure such as plenty of primary lysosomes,agglomeration of lipochrome,Golgi complex degeneration and so on.

结果上述核团神经元的超微结构均发生病理性改变,神经细胞核膜皱缩,核膜凹凸不整,并有局部断裂;线粒体变性,基质浓度降低及空泡化;粗面内质网和高尔基复合体囊腔扩张变性;大量初级溶酶体及脂褐素集聚;出现了髓样体和多泡体等变性结构。

Experimental study indicated: the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats, promote its multiplicative division, and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-α and the osteoblast senescence of rats induced by t-BHP.

实验研究表明:骨碎补总黄酮能够提高大鼠骨髓基质细胞的活性,促进其增殖分裂,并对脂质化大鼠骨髓基质细胞凋亡具有保护作用,而且骨碎补总黄酮含药血清对TNF-α诱导的大鼠成骨细胞凋亡和t-BHP诱导的大鼠成骨细胞衰老均具有一定的保护作用。

The increased amount of non--exchangeable NH4^+-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^+-N (P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively). NH4^+-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^+-N increased significantly when added (NH4)2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^+-N from (NH4)2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^+-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d (P=0.0434), but not significantly at 40 d and 60d (P=0.7378 and 0.5375). The increased amount of non-exchangeable NH4^+-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^+-N, the increased amount of non-exchangeable NH4^+-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^+-N的增加量均表现为以土垫旱耕人为土最大,其次是黄土正常新成土,简育干润均腐土和干湿砂质新成土较小;添加有机物料极显著影响培养期间的非交换性NH4^+-N增加量(培养20d、40d和60d时P分别为0.0002,0.004和0.0003),表现为紫花苜蓿和长芒草土壤非交换性NH4^+-N的增加量均极显著高于不添加有机物料的对照土壤;在培养20d、40d和60d时,加(NH4)2SO4土样非交换性NH4^+-N的增加量显著大于不加(NH4)2SO4土样(户分别为0.0037,0.0033和0.0027),这是土壤对(NH4)2SO4中NH4^+-N固定的必然结果;不同植被类型土壤培养20d时的非交换性NH4^+-N增加量差异显著(P=0.0434),培养40d和60d时差异不显著(p分别为0.7378和0.5375);长期秸秆和氮肥配施土壤非交换性NH4^+-N增加量大于不施肥对照土壤,但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^+-N含量均呈极显著正相关;而非交换性NH4^+-N的增加量与粘粒无相关性,但与全氛和有机质呈显著正相关。

Results: Of all the semen samples, 8 were HCMV positive, 4 HSV-Ⅱ positive, but none were both HCMV and HSV-Ⅱ positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-Ⅱ positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration.

结果:83例精液样本PCR检测HCMV阳性8例,HSV-Ⅱ阳性4例,未发现2种病毒同时阳性病例。8例HCMV阳性病例ICC标记生精细胞HCMV晚期抗原均见阳性表达,HCMV早期抗原均为阴性表达;4例HSV-Ⅱ阳性病例ICC标记生精细胞3例阳性表达,1例弱阳性。12例病毒阳性病例均发现较多未成熟生精细胞,并有不同程度的核染色质固缩、出现空泡、核膜破损、核崩解、凋亡小体等凋亡现象,但未能找到病毒感染的特异性形态学改变。

Chromatic plastic film and light conversion film has been applied in protected cultivation, and plastic films with different light quality may serve as a non-chemical approach regulating growth and thermoperiodic responses of plants. A few studies showed that light quality affected growth and yield of strawberry, but little is known about its effects on photosynthesis and chlorophyll fluorescence in strawberry leaves, and fruit quality. So it is beneficial to scientific regulating growth and fruiting for strawberry protected cultivation to study on those effects and the interactions between CO2 and N supply on photosynthesis.

而低氮影响植株生长、叶片黄化,进而影响光合作用,导致抗性减弱和产量品质下降;③CO_2供应问题:目前我国草莓栽培常在设施环境下进行,在封闭性强的设施中空气流通不畅,常导致设施内部CO_2供应不足而影响光合作用,进而影响产量和品质;④光不足与光质问题:设施栽培中,光通过玻璃和塑料棚膜后使设施内的光强与光质均发生改变,这些变化特别是光质变化对草莓生长与结果的影响程度尚无明确认识。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用:主要表现为:(1)对受损神经元具有直接的保护作用,可提高受损神经元的活性和存活率,降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量;(2)对受损的星形胶质细胞也有直接的保护作用,可提高其活性、存活率以及培养液蛋白质的含量;(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力;(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达,降低受损神经元对bax和caspase-3的表达;(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元,因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用,统计学上具有显著性差异。

Makes the decency coal, the pearlite and the vermiculite carries on between the root nodulebacteria and the alfalfa variety match as the matrix, alfalfas nitrogen fixation effect and thegrowing trend is higher than other two kind of matrix, the decency coal may first take the highlyeffective strain screens matrix.

以风化煤为基质进行根瘤菌与苜蓿品种之间的亲和性的匹配试验中,苜蓿的固氮效果和长势均高于珍珠岩和蛭石这两种基质。因此,风化煤可优先作为高效菌株筛选的基质。

Results Brucine and its liposome had significant inhibiting effect on transplanted tumor growth in mice. Inhibitory rates of brucine on Heps were 35.05% [1.61mg/], 43.70% [3.23mg/], and 46.09% [6.46mg/], while of its liposome on Reps was 45.41% 1.61mg/1 and 58.19% [3.23 mg/, respectively. The inhibitory rate of brucine on S180 was 37.59% [1.61 mg/], 36.13% 3.23mg/, and 38.87% [6.46 mg/, while of its liposome on S180 was 53.00% [1.61mg/] and 48.37% [3.23mg/], respectively. However, both of them had no distinct effect on prolonging the life of mice with transplanted tumor.

结果 马钱子碱及其脂质体能有效抑制实体瘤模型荷瘤小鼠体内肿瘤生长,1.61、3.23、6.46mg/马钱子碱对Heps小鼠的抑瘤率分别为35.05%、43.70%和46.09%,而1.61、3.23mg/马钱子碱脂质体的抑瘤率分别为45.41%和58.19%;1.61%、3.23、6.46mg/马钱子碱对S180小鼠的抑瘤率分别为37.59%、36.13%和35.57%,而1.61和3.23mg/马钱子碱脂质体的抑瘤率分别为53.00%和48.37%,明显高于马钱子碱;但两者对腹水瘤模型荷瘤小鼠的生存时间均无延长作用。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus, BFV)中国株BFV3026原病毒DNA为材料,构建R区系列缺失质粒,通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定,确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用;同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下,通过对其转染细胞293T中RT酶活性的测定,确立R区对于病毒结构基因pol的表达具有一定的调节作用,并将其功能区域初步界定在R区5′端100bp内。

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