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The double membrane vesicle, which was produced by budding of the outer membrane of amyloplast, accumulated starch to form new amyloplast. The double membranes of amyloplast invaginated and the inner membrane dilated to form new amyloplast in the amyloplast.

淀粉质体增殖产生新淀粉质体有多种方式:出芽增殖、缢缩增殖、中间隔板增殖、被膜向内出泡或内陷增殖、被膜形成双层膜小泡再积累淀粉增殖,它们均是淀粉质体被膜的一种膜行为。

They were diagnosed as chronic tubulointerstitial nephropathy accompanied with renal tubular acidosis, renal glucosuria, hyposthenuria and different degree of chronic renal dysfunction.

结果:9例均为中老年患者,临床上均表现为慢性小管间质性肾病,均有肾小管酸中毒、肾性糖尿、低渗尿和不同程度的慢性肾功能减退。

In the group of treatment with ZKK,the AST,ALT and the lipid in the liver decrease significantly and differ from the natural recovery group'sbut the normal group's.In the group of treament with Simvastatin,the affection to the ALT,AST and the lipid in liver is no apparent,there is no difference campared to that of recovery group,but very difˉferent from that of treatment group with ZKK,In the combined treatment group,the effect to decrease the bloodlipid is significant,the influence on liver is similar to the ZKK's.Conclusion Rich-fat food can induce dyslipidemia,atheromatous patch and hepar adiposum.

辛伐他汀组对成模时增高的ALT、AST和肝组织中的脂质及脂肪肝影响不大,与模型组相比无差别;之可康组和联合治疗组均可明显降低成模时升高的AST和ALT及肝组织中的脂质,逆转脂肪肝,与正常组相比无差别,与模型组、辛伐他汀组分别相比差异有显著性;联合用药组血脂水平调节幅度最大,对肝脏的影响与之可康组相比无差别。3个治疗组均可使动脉硬化斑块回缩。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长,以少突胶质细胞表现更为明显;(2)星形胶质细胞呈向心性生长,细胞相邻的接触面有较多的伪足;(3)传代培养的星形胶质细胞保持较强的分裂增殖能力,可在1周左右生长至完全融合,其纯度可达95%以上。

But the latent period for reaching the platform above the water in Morris Water Maze of the Mn-exposed group was not different from that of control group. There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

The physicochemical properties, fatty acid composition and mineral elements of Viburnum cylindricum seed oil were determined. The results showed that 20 kinds of fatty acids in the Viburnum cylindricum seed oil were identified, in which the main components were oleic acid (33.34%), 8,11-octadecadienoic acid (28.24%) and palmitic acid (22.55%); the seed oil contained 6 kinds of essential mineral elements including Ca, P, Mg, Fe, K and Mn, and their contents were in the reasonable range of body requirement.

测定了水红木油的理化指标;用GC/MS法测定了脂肪酸组成及含量,共检出20种脂肪酸,主要成分9-十八碳烯酸占33.34%,8,11-十八碳二烯酸占28.24%,十六烷酸占22.55%,3种脂肪酸共占84.13%;用ICP-OES法测定了矿质元素的种类和含量,检出的Ca、P、Mg、Fe、K、Mn 6种矿质元素均为人体必需元素,含量均在人体所需合理范围内。

Methods by using a broad pH range column(Gemini NX C18,4.6mm×150mm,5μm),basic mobile phase which was[H_2O-ammonia-glacial acetic acid] (96:3.6:0.4)-methanol,the related substances in etimicinsulfate were separated and detected by electrospray ionization and positive ion monitoring,the ESI ion source condition was following: temperature is 350℃,nebulizing pressure is 50 psi,dry gas flow is 10 L /min.some related substances in etimicinsulfate were identified by comparing the retention time in chromatography,~+ spectrum and MS~2 spectrum with reference substances\',the others which haven\'t reference substances were identified or speculated by analyzing their MS~2 fragmentation with the help of a rule summarized from the MS~2 fragmentation of gentamicin C1a,micronomicin and etimicin.Results nineteen related substances in etimicinsulfate were separated and detected.

方法利用宽pH范围的Gemini NX C18(4.6mm×150mm,5μm)色谱柱,在碱性条件下,以[水-氨水-冰醋酸(96:3.6:0.4)]-甲醇(70:30)为流动相,直接分离各组分;并用HPLC-ESI-MS~2检测样品中各组分,质谱条件:ESI离子源,离子源温度350□,雾化室压力50 psi,干燥气流速10 L/min,正离子检测方式;对有对照品的有关物质采用比对有关物质与对照品的色谱保留时间、准分子离子的质荷比及二级质谱方法进行鉴定,无对照品的有关物质,则是先通过解析庆大霉素Cla、小诺霉素、依替米星的二级质谱,对该类化合物的二级质谱裂解规律进行归纳,并以此推测未知新化合物进行结构,再用推测的结构对二级质谱进行解析,如所有的碎片离子均合理解释,表明推测合理。

Almost all CF was released from Dansyl-PE by CTX-IX that osmosed the Dansyl-PE liposome like detergent; CF releases from PE or PS liposome induced by CTX-IX were related with both of CTX-IX and phospholipid concentrations, this indicated that the phase seperation should be a reasonable mechanism for CF permeability of PS or PE liposome in the presence of CTX-IX rather than CTX-IX channel formation on the membrane. CTX-IX influence of CF release of PC liposome was very slight. CF release from Dansyl-PE、PE or PE liposome in response to CTX-IX could be inhibited by Ca〓.

CTX-IX对靶脂如此种种强烈的作用,使得它引起含Dansyl-PE脂质体CF的泄漏犹如击污剂那样强烈;引起含PS或PE脂质体CF渗透的增加为一个与CTX-IX及磷脂浓度有关的二级反应过程,这种动力学特征提示渗透性增加的机制可能是CTX-IX在脂膜上产生的脂质分子相分离,而不是CTX-IX脂膜上形成通道;CTX-IX对PC脂质体渗透性几乎没有影响,而对上述脂质体渗透性增加作用均可被Ca〓所抑制。

Results: Tubulointerstitial damage index, tubulointerstitial fibro- sis index, and expression of TGF-β1 in group U were significantly higher than those in group S (P.01). These indexes were obviously lower in group A than those in group U (P.05). TDI and TFI positively correlated with expression of TGF-β1 respectively.

结果:U组肾小管间质损伤指数及肾间质纤维化指数均明显高于S组(P.01);U组TGF-β1的表达均明显高于S组(P.01);A组的上述各指标分别较U组降低(P.05);TDI、TFI分别与TGF-β1的表达呈正相关。

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