均质的

Wtp53 has obvious antiblastic effect towards Y79 cells, and the apoptosis rate of Y79 cells was the highest after transfection mediated by ultrasound-microbubble.

结论wtp53基因对RB瘤细胞具有较明显的抑制生长的作用；质粒+微泡+超声辐照，质粒+脂质体转染，以及质粒+超声辐照，均可促进Y79细胞的凋亡，但超声微泡介导的转染引起的凋亡率高于脂质体转染，而二者的凋亡率又明显高于质粒+超声转染。

Results The hind limb function of the injured rats recovered at different degrees, the most extent recovery occurred during the time site 1~2 week, recovery continued from 2 to 3 week and, BBB was up to 12 at the end of the third week, but there was no significance recovery during 3~4 week. the astrocyte caudal to the injury plane began hyperplasy and hypertrophy; astrocyte in which GFAP in expression was positive the gray matter increased obviously from 3 days to 14 days after hemisection. The expression of MBP is same as that of GFAP.

结果 伤后后肢均有不同程度的恢复，1~2周时恢复幅度最大，2~3周时后肢运动功能继续恢复，3周时BBB评分最高达12分，3~4周运动功能无显著性恢复，损伤后1 d损伤远端3~6 mm处GFAP阳性星形胶质细胞开始增生肥大，3~4 d灰质中星形胶质细胞明显增多，2周时达到高峰，损伤近端3~6 mm处少突胶质细胞的增生反应过程与星形胶质细胞相似。

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d，D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9，从用药后14 d开始明显缩小(均P＜0.05)；而其他3组瘢痕疙瘩组织块体积均明显增大，从用约后7 d开始各时点测得的体积均明显大于D组(均P＜0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润，胶原结构破坏和成纤维细胞凋亡明显重于其他3组，Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

Compared with the comparison group,the number of children who have positive history of hypersusceptibility and familys disease are markedly different. 2.Compared with the comparison group, the number of children not only between the congruity constitution and non-congruity constitution,but also between the Fei-Pi constitution and Pi-Shen constitution have significant difference.AH the children in CVA group are non-congruity constitution types.Of all the children with Fei-Pi constitution types,there is much more distributing in CVA group than that of comparison group. 3.The criterion of constitution types which is adopted in this research can be recommended in some degree and area. 4. there is a hypothesis that probably the particular constitution which caused the CVA in the episode of CVA.

1、与对照组相比，CVA患儿有过敏史及相关疾病家族史人数比较差异极其显著。2、两组儿童调和质人数和非调和质人数比较差异极其显著，CVA组患儿均为非调和质；CVA组和对照组儿童非调和质中A、B型差异极其显著，CVA组B型体质分布偏多，CVA组与对照组肺脾质儿童中也以CVA组B型体质分布居多。3、本研究所采用的体质分型标准在CVA患儿的体质研究中可以在一定范围内一定程度上采用。4、CVA患儿病因上可能存在体质发病途径。

Results (1)The permillage of myocyte apoptosis in infarct area in elder patients with sudden death caused by AMI was significantly higher than that in control group(663.00‰±117.12‰ vs 34.30‰±20.68‰,P＜0 .01).However the permillage of myocyte apoptosis in 1-vessel,2-vessel,and 3 - vessel disease were 514.28±165.12‰,564.38‰±102.33‰ and 668.25‰±127. 19‰,respectiverly,significantly higher as compared to control group(All P＜ 0.01).But no significance was found among the three groups.(2)The size of DNA f ragment about 180～200 bp was found only in those patients with two and three ve ssels involoved.(3)The electron microscope showed the characteristics of myocyte apoptosis episodes,the others showed the characteristics of necrosis.

结果 TUNEL法发现，猝死者梗死区的心肌细胞凋亡千分数老年组(663.00‰±117.12‰)明显高于正常对照(34.30‰±20.68‰)(P＜0.001)；心肌细胞凋亡千分数在冠脉1支病变者(514.28‰±165.12‰)＜2支病变者(564.38‰±102.12‰)＜3支病变者(668.25‰±127.19‰)，虽然均明显高于正常对照(均P＜0.01)，但三组之间比较则无统计学上的差别(均P＞0.05)；冠脉2～3支病变者梗死区的心肌细胞DNA电泳可见相差约180～220 bp的阶梯片段；电镜发现猝死者梗死区内的心肌细胞核膜完整、染色质浓集、电子密度增加的凋亡特征，有的则出现核膜破裂、染色质溶解成碎屑的坏死现象。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The results showed the bacterial supernatant and periplasm containing NKAT2 scFv were able to bind the NKAT2 receptors expressed on cell surface,but NKAT4 scFv failed to bind to NKAT4 receptors.

结果显示，含NKAT2－scFv的细菌上清及胞质提取物均可识别到表达在细胞表面的NKAT2受体分子，但含NKAT4－scFv的细菌上清及胞质提取物均未成功

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。