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The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

结果 获得1株含HIV-1 gp41基因的重组质粒,其蛋白的特异性表达为8%,经HIV-1阳性血清检测和国家标准参比血清检定,该表达蛋白灵敏性、特异性均为100%。

Result and Conclusion: The optimum proportion of PA-MC-1, kaolin, lipa is 90∶10∶0.5, and join extracts of Chinese traditional medicine,then make into CTM Poultice of Neck-Shoulder-Waist-Leg paste, its viscosity moderate, spread-stretchability and moist-proof quality are very good.

结果与结论:PA-MC-1,高岭土,油脂的配比是90∶10∶0.5为最优,并以该基质加入中药提取物制成颈肩腰腿一贴灵的中药巴布剂,其粘性适度,涂展性,保湿性均良好,经临床验证,对皮肤无刺激性与过敏性,且疗效显著耐久。

Field trials on the effect of three levels (1.0, 1.5 and 2.0 kg/plant/year in plant cropping, and 0.76, 1.06 and 1.36 kg/plant/year in ratoon cropping) of compound fertilizer Tai-Fei No.4 on the vegetative growth, yield and post-harvest quality attributes of TC1-229, a semi-dwarf, fusarial wilt-resistant banana clone, were conducted on a soil of silt loam with a pH of 8.0 and 2% of organic matter for two consecutive years.

在有机质含量约百分之二的坋质壤土定植中矮性抗黄叶病品系TC1-229组织培养苗蕉株,每年每株施用台肥四号复合肥料1.0,1.5或2.0公斤,抽穗期叶片之矿物营养组成均在正常范围内,惟磷和镁含量随肥料施用量减少,处理间差异达显著水准(P=0.05)。

Base on the properties mention above, they are very similar with the Ando soils of Japan or Indonesia Java. But different from Black colour soils in Eastern Taiwan, black soils derived from basalt in the North and Rendzina in the South. That may be called as black color soils of andesite.

阳明山之黑色土各项理化特性与日本或爪哇之Ando Soils相近,但母质并非火山灰,而为安山岩或安山凝灰岩,与木省东台湾之黑色土,或北部玄武岩之黑色土,或南部之黑色石灰土之理化性均有不同,笔者认为称应之为黑色安山岩土。

Note: pregnancy, lactation, severe liver damage, jaundice, or a continued itching during pregnancy, Dubin-Johnson syndrome, Rotor syndrome, liver cancer or a history of liver tumors, thromboembolic, or a history of sickle-cell anemia, breast cancer or endometrial cancer, vascular changes associated with severe diabetes, lipid metabolism disorders, should be used with caution.

注意事项:妊娠,哺乳,严重的肝功能损害,黄疸或在妊娠期有持续瘙痒,Dubin-Johnson综合征,Rotor综合征,肝脏肿瘤或有肝脏肿瘤病史,血栓栓塞或病史,镰状细胞性贫血,乳腺癌或子宫内膜癌,伴有血管变化的严重糖尿病,脂质代谢紊乱,均应慎用。

Sinensis were conducted.The result of SDS-PAGE showed that different parts in spermatophore, including spermatophore matrix, seminal plasma and the whole sperm, had different characteristic bands in PAGE maps, and the result could be repeated, so the method for spermatophore fractionation was reliable.

实验结果显示,通过分级分离所得的精液、精荚基质和纯品精子等各个部分,在SDS-PAGE电泳图谱上均显示出不同的特征性谱带,且试验的重复性较好,证明本实验建立的精荚分级分离的方法是可行的。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明,SM2-Kit的标准曲线呈典型的S型,相关系数R2=0.9911,符合4参数logit曲线拟合,线性检测范围为0.625 μg/L~80 μg/L,灵敏度为0.9 μg/L,半数抑制浓度(IC50)为5.82 μg/L,检测限为1 μg/L;饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %,平均批内和批间变异系数均低于15 %;SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %,与其它磺胺类药几乎没有反应性;基质对SM2-Kit的检测结果影响不大;试剂盒在4 ℃可保存6个月。

At present the urgent question affronting the medical scinence field is to seek effective medical for pulmonary fibrosis.

肺间质纤维化,可分为特发性和继发性两大类型,虽由多种病因引起,但均具相似的病理过程。

In this study, EPMA and LA-ICP-MS were used to determine the major and trace element concentrations, respectively, of apatites from Transhimalayan granitoids. The results indicate that F, Mn, Sr and REEs in apatites generally show good correlations with compositions of their host magmas and thus have high potential to be utilized as petrogenetic tracers. More specifically, F and Mn contents in apatites are covariant with the aluminosity of the host rocks so that can be used as an indicator for magma differentiation. Combining with Sr and REE data, which show significant variations in apatites from different rock types, these elements may be furthermore used to construct "discrimination diagrams" for more detailed investigations of complex petrogenetic processes such as magma mixing and compositional heterogeneity.

本研究中,电子微探以及雷射烧蚀感应耦合电浆质谱术分别被用以量测藏南火成岩中磷灰石的主量与微量元素含量,微区分析的结果显示,磷灰石的氟、锰、锶以及稀土元素均与岩浆源成分展现良好的对比关系,揭示了它们应用在岩石成因示踪上的优秀潜力:其中,磷灰石氟、锰含量的变化与原岩的铝饱和情形息息相关,因此可以作为岩浆分异程度的指标;而锶以及稀土元素则在不同岩石类型来源的磷灰石中展现了明显的丰度变化,因此综合这些元素将有机会进一步建立岩浆来源的「鉴别图」,甚而以此讨论岩石成因,例如:岩浆混合与源区组成的不均一性。

Recombinant apoE〓 protein could inhibit the death of the neurocyte cell of mice brain induced by beta amyloidal peptide 25-35 fragment and the death of the SK-N-SH-neuroblastoma cell induced by beta amyloidal peptide 25-35 fragment and 1-40 fragment.

重组apoE〓蛋白对Aβ 25—35多肽诱导的胎鼠大脑皮质神经细胞凋亡及Aβ25-35和A β1-40多肽诱导的神经成纤维胶质瘤细胞生存力下降均具有明显的拮抗作用,表明apoE对神经退化性疾病中的细胞死亡具有拮抗作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。