均质

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity.

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法。

Results: MMP-9 and TIMP-1 were mainly expressed in the cytoplasm in tumor cells, and rarely in tumor stroma.

结果：MMP-9和TIMP-1蛋白在肿瘤细胞和间质细胞均有表达。

The expression of MMP-3 was higher than that of MMP-9 in the stroma of the endometriotic tissues.

在异位内膜的间质和腺体，MMP-3染色均较MMP-9增强(P＜0.01 P＜0.05)。

MMP-9 was detected in both pancreatic cancer cells and stroma.

MMP-9在胰腺癌细胞和间质组织中均有表达。

The expression of ER-α was in endometrial epithelium and stroma locating in nucleus.

ER-α在子宫内膜腺上皮和间质均有表达，定位于细胞核。

Methods:3-exceptions health brainstem glioma one case of the pterional,two cases of the sigmoid sinus after suboccipital approach adopted microscopic surgery,postoperative radiotherapy governance week trip gamadao treatment.

3例外生型脑干胶质瘤1例经翼点入路，2例经枕下乙状窦后入路均采用显微外科手术，术后一周行伽马刀放射治疗。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Basalts plot in tholeiite in A.I-Al〓O〓 diagram. They have enriched LREE patterns without negative Eu anomaly. Their Zr/Y=4.4～6.5,〓=0.70790～0.70146, Th＞＞Ta,εNd values are between -1.8 and +3.7, indicating continental contamination. Pb isotope characteristics are close to that of typical tholeiitic flood basalt in the world.

Al〓O〓图上落入拉斑玄武岩区；稀土配分为LREE富集型，均无负Eu异常，Zr/Y＝4.4-6.5，〓＝0.70790～0.70146；Th＞＞Ta，ε〓介于-1.80～+3.7之间，都显示了陆壳混染的影响，Pb同位素与世界上典型拉斑质溢流玄武岩比较接近。

Objective:The aims of the present study were to determine the expression of transformation growth factor β 1 (TGF β 1) mRNA and TGF β 1 protein in the peripheral blood and its relation to serum levels of procollagen type Ⅲ N terminal peptide in the patients with cirrhosis.

转化生长因子-β1(TGF-β1)在一些体外和体内的研究中均显示能刺激胶原和细胞外基质的合成，同时又可通过抑制ECM降解以加强纤维的发生，在肝纤维化中起重要作用。

There were no significant differences in soil bacterial and fungal biomass, total nematode density and diversity, herbivore density, bacterivore density and omnivore-predator densities between the trenched and control plots in both mineral and humus soil layers.

切根处理对腐殖质层和矿质土层的细菌和真菌生物量、线虫总密度和多样性、植食性线虫、食细菌线虫和捕食与杂食性线虫的密度均没有显著影响。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。