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Further study indicated that low dose breeding with diphenylene iodonium or apocynin, two inhibitors of NADPH oxidase,significantly suppressed ROS release caused by aerobic exercise.

和H2O2生成均明显增加,而人工饲喂NADPH氧化酶的抑制剂二联苯碘(diphenylene iodonium,DPI)或夹竹桃麻素(apocynin,APO)后,均明显地降低由运动引起的大鼠神经胶质细胞O2^-。

We found that the release of ROS in rat astrocytes was significantly increased by the stimulation of calcium ionophore or opsonized zymosan, which are known to trigger respiration burst in phagocytes by NADPH oxidase pathway. Further study indicated that diphenylene iodonium or apocynin , two inhibitors of NADPH oxidase, significantly suppressed ROS release caused by calcium ionophore or opsonized zvmosan.

我们发现在用吞噬细胞呼吸爆发刺激剂钙离子载体或酵母多糖刺激星形神经胶质细胞时,细胞O〓和H〓O〓产量均明显增加,而NADPH氧化酶的抑制剂二联苯碘(diphenylene iodonium,DPI)或夹竹桃麻素(apocynin,APO)均可以明显地降低刺激剂引起的细胞O〓和H〓O〓产量增加。

After selective extraction, the cyst wall of two kind of resting cysts displayed a filament network. In the resting cysts of Tachysoma pellionella and Euplotes encysticus, the filament was located in mesocyst and endocyst respectively. On the other hand, a special filament scaffold region was observed below the cyst wall in two kind of cysts. Compared with the true IF which was located in central part of cyst, the filament of this region was net as well as uniform, and no any cytoplasm residue can be seen in this region. In the resting cyst of Euplotes encysticus, a filamental boundry which partitioned the special filament scaffold region and the true IF region was observed.

两类休眠包囊的包囊壁经生化分级抽提后均显示纤维结构,急纤虫休眠包囊中纤维定位于中间层,包囊游仆虫休眠包囊中纤维定位于内层壁;其次,在两类休眠包囊的包囊壁下,相当于细胞质表质层的位置,均有一个特殊的纤维网络区,与细胞质深层的网络区相比,此部分的纤维纯净、均匀,未见附着细胞器及细胞质残留物,在包囊游仆虫休眠包囊中此纤维网络区与真正的细胞质纤维网络之间由纤维构成的界膜隔开。

Among them, 39 cases were in low grade ,31 cases in high grade and 4 cases had the ependyma tumor. All the patients received radiotherapy in 3-4 weeks after operation. Thirty-one cases were performed total resection, 38 cases balk-resection and 5 cases partial resection.

对脑胶质瘤低级别39例、高级别31例及室管膜瘤4例均进行手术切除,其中肉眼肿块全切31例,大部切除38例,部分切除5例,均在手术后3~4周内接受放射治疗。

Compared with the control, pear with S2, S5 and PDR54 as inter rootstock bore fruits ahead of 2-3 years .Pear with PDR54 fruited even more early.

以PDR54为中间砧的嫁接树叶片当中,N、P、K、Ca、Mg、Fe、Zn等所测定的矿质元素的含量均有不同程度的下降,其中K和Ca的降低程度均超过了10%。

With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长,表达的融合蛋白产量逐渐增加,表达蛋白总量最高可达到菌体总蛋白的39.14%;在15℃、25℃和37℃三个不同的诱导温度下,均能诱导产生融合蛋白,而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加;在菌体的各个部位中,融合蛋白主要在细胞质中以可溶的形式进行表达,有少量在外周质中表达或以包含体形式在细胞质中表达。

Three mice were killed every time at interval, of one, two, three, four, and six weeks after the implantation, and the tissue where the composite was implanted was excised for histologic investigation of heterotopic steogenesis under photomicroscopy.

结果:大鼠骨髓基质细胞体外培养后增殖,植入后2周(1只)、3周(2只1)、4周(1只)、6周(1只),在石膏和陶瓷人工骨复合移植侧载体周边均见骨生长、骨软骨生长和骨髓生长,各阶段中均见纤维组织增生。

In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21, The expression of recombinant prolyl endopeptidase in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains.

在高密度发酵条件下,G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重,均明显优于对照菌株PA1和BL21;重组蛋白脯氨醯内肽酶在G830和PA1中获得稳定高表达;重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。

Take silica gel chromatography eluting with EtOAc / petroleum ether(1:9~5:5). The production was washed several times by MeOH, and dissolved in dimethyl formamide to recrystallize, we got yellow power crystal.

结果:(1)HPLC检测含量大于95%,符合对照品的要求;(2)理化鉴别实验均与文献吻合;(3)紫外、红外、质谱、核磁共振的光谱结果均与文献值一致,确定产物为金松双黄酮。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

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