均质

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d，D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9，从用药后14 d开始明显缩小(均P＜0.05)；而其他3组瘢痕疙瘩组织块体积均明显增大，从用约后7 d开始各时点测得的体积均明显大于D组(均P＜0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润，胶原结构破坏和成纤维细胞凋亡明显重于其他3组，Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状，由精子群、精荚基质及初级精荚壁和次级精荚壁组成，初级精荚壁与精荚基质是相连续的，它们之间无明显界限，两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成，但后者不含精子，后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物，可能与形成精子细胞核中的泡状带和膜状带有关，在交配后的雌虾，其头胸部腹面上粘着的精荚中，其基质内已不见絮状和同心圆泡，仅见稀疏的纤丝。

When EGTA (Ca〓 chelator), Verapmil and LaCl〓(Ca〓 channel blockers) were used to treat tomato fruit at green mature and strawberry fruit at white stage with ethylene, they could reverse ethylene-induced increase in ethylene production in tomato and strawberry, PG activity, lycopene content, soluble protein content in cell wall in tomato and degradation in soluble protein in strawberry, which indicated blocking Ca〓 channel in plasma membrane or chelating Ca〓 can decrease intracellular Ca〓, further inhibite ethylene-induced maturation, ripening and senescence of fruit.

用质膜钙通道阻断剂异博定、钙通道Ca〓竞争性抑制剂以及Ca〓专一性螯合剂与乙烯一起处理绿熟期番茄果实和乳白期草莓果实，均可抑制乙烯诱导番茄和草莓的乙烯生成、番茄PG活性的提高、番茄红素含量的增加和细胞壁可溶性蛋白含量的增加以及草莓可溶性蛋白的分解，表明阻断质膜Ca〓通道或螯合胞外Ca〓以减少胞内Ca〓能抑制乙烯对果实的催熟作用，间接证明乙烯对果实成熟衰老的诱导与胞外Ca〓内流引起的胞质Ca〓浓度的增加密切相关，表明乙烯信号转导可能与钙信使有关，调节胞质Ca〓浓度可调节乙烯对果实的催熟作用。

objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.

目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验，取50只小鼠随机分成5组，分别给予溶媒和不同剂量的色素，然后采用溴代苯致实验性肝损伤，测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用，抑制率随样品的浓度增高而增大，并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中，中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。

By electrospray ionization multistage mass spectrometry, the fragmentation pathways of ＋ and ＋ions of homocyclic peptides were studied in detail. The fragmentation pathways of ＋ ions were different from those of ＋ ions and their dissociation mechanisms were proposed, respectively.

采用电喷雾多级质谱技术，对系列均环多肽＋离子和＋离子的质谱裂解规律进行了系统研究，两种系列的离子具有不同的质谱裂解特征，分别提出了其可能的质谱裂解机制。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The increased amount of non--exchangeable NH4^＋-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^＋-N （P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively）. NH4^＋-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^＋-N increased significantly when added （NH4）2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^＋-N from （NH4）2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^＋-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d （P=0.0434）, but not significantly at 40 d and 60d （P=0.7378 and 0.5375）. The increased amount of non-exchangeable NH4^＋-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^＋-N, the increased amount of non-exchangeable NH4^＋-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^＋-N的增加量均表现为以土垫旱耕人为土最大，其次是黄土正常新成土，简育干润均腐土和干湿砂质新成土较小；添加有机物料极显著影响培养期间的非交换性NH4^＋-N增加量（培养20d、40d和60d时P分别为0.0002，0.004和0.0003），表现为紫花苜蓿和长芒草土壤非交换性NH4^＋-N的增加量均极显著高于不添加有机物料的对照土壤；在培养20d、40d和60d时，加（NH4）2SO4土样非交换性NH4^＋-N的增加量显著大于不加（NH4）2SO4土样（户分别为0.0037，0.0033和0.0027），这是土壤对（NH4）2SO4中NH4^＋-N固定的必然结果；不同植被类型土壤培养20d时的非交换性NH4^＋-N增加量差异显著（P=0.0434），培养40d和60d时差异不显著（p分别为0.7378和0.5375）；长期秸秆和氮肥配施土壤非交换性NH4^＋-N增加量大于不施肥对照土壤，但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^＋-N含量均呈极显著正相关；而非交换性NH4^＋-N的增加量与粘粒无相关性，但与全氛和有机质呈显著正相关。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

Compared with the control, the lungs of 3-day-hyperxia-exposure group developed into edema, hemorrhage and inflammatory infiltration and this condition aggravated at the 7th day. The pulmonary septum was significantly widened at the 14th day, the interstitial fibrocytes as well as alveolar epithelial cells proliferated obviously, and the tendency of lung fibrosis was also presented. The results mentioned above showed that the modle of hyperoxia lung injury was successfully established, and the acute inflamationm was its early symptom while the fibrosis was its late manifistation. There was no positive cytes of IFN-γ in the lung of nomal rats, The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day, which climbed to the climax at the 7th day and began to decline from the 14th day, were found in alveolus epithlia,pulmonary interstitial, bronchoepithlia and macrophage.

高氧14天（5.46±0.30）虽有所下降，但同空气组比，差异仍然具有显著性（P.05）；BALF中蛋白含量高氧3天（0.45±0.10）、7天（0.66±0.08）、14天（0.79±0.14）显著高于空气组（0.18±0.08，P值均。05）；肺通透系数高氧3天（6.61±2.05）、7天（10.88±1.54）、14天（12.75±2.20）均明显高于空气组（2.99±1.27，P值均。05）；同时病理组织学改变也表明与空气组比较，高氧3天时肺组织水肿、出血、炎性细胞浸润，7天时进一步加重，14天时肺间隔明显增宽，间质纤维细胞和肺泡上皮细胞明显增生，出现肺纤维化倾向；以上结果均表明我们成功地建立了高氧肺损伤动物模型，且急性炎症反应是其早期表现，纤维化改变是其晚期表现。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。