均质

Wtp53 has obvious antiblastic effect towards Y79 cells, and the apoptosis rate of Y79 cells was the highest after transfection mediated by ultrasound-microbubble.

结论wtp53基因对RB瘤细胞具有较明显的抑制生长的作用；质粒+微泡+超声辐照，质粒+脂质体转染，以及质粒+超声辐照，均可促进Y79细胞的凋亡，但超声微泡介导的转染引起的凋亡率高于脂质体转染，而二者的凋亡率又明显高于质粒+超声转染。

Compared with the comparison group,the number of children who have positive history of hypersusceptibility and familys disease are markedly different. 2.Compared with the comparison group, the number of children not only between the congruity constitution and non-congruity constitution,but also between the Fei-Pi constitution and Pi-Shen constitution have significant difference.AH the children in CVA group are non-congruity constitution types.Of all the children with Fei-Pi constitution types,there is much more distributing in CVA group than that of comparison group. 3.The criterion of constitution types which is adopted in this research can be recommended in some degree and area. 4. there is a hypothesis that probably the particular constitution which caused the CVA in the episode of CVA.

1、与对照组相比，CVA患儿有过敏史及相关疾病家族史人数比较差异极其显著。2、两组儿童调和质人数和非调和质人数比较差异极其显著，CVA组患儿均为非调和质；CVA组和对照组儿童非调和质中A、B型差异极其显著，CVA组B型体质分布偏多，CVA组与对照组肺脾质儿童中也以CVA组B型体质分布居多。3、本研究所采用的体质分型标准在CVA患儿的体质研究中可以在一定范围内一定程度上采用。4、CVA患儿病因上可能存在体质发病途径。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长，以少突胶质细胞表现更为明显；(2)星形胶质细胞呈向心性生长，细胞相邻的接触面有较多的伪足；(3)传代培养的星形胶质细胞保持较强的分裂增殖能力，可在1周左右生长至完全融合，其纯度可达95%以上。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

objective to compare image manifestations values between high resolution computerized tomography and x-ray chest film in idiopathic interstitial pneumonia in the elderly.methods we analysed and compared image manifestations between hrct and x-ray chest film with 12 cases of clinically suspected iip in the elderly.results ct and hrct had more sensitivity and specificity than x-ray chest film.there were more image manifestations in hrct such as grand grass,net,line,nodular,honeycombing,bronchiectasis,honeycombing cyst,peribronchovascular interstitial thickening and irregularity.there were significant difference between them （p.05）.these patients who were suspected iip had better control and remission after being treated with hormone and immuno-suppressive drug.conclusion it have more significant virtues in hrct to manifest iip in the elderly than x-ray chest film,and it also may manifest iip curative effect.

目的 比较高分辨断层摄影术、x线胸片检查对老年特发性间质性肺炎的诊断价值。方法对12例临床疑诊为间质性肺炎的老年患者，进行胸部hrct与胸部平片检查，并对照分析。结果胸部ct在诊断方面比常规胸片有较大的优越性，hrct则有更高的敏感性和特异性，hrct表现出磨玻璃影、网状、线条、小结节、蜂窝影，支气管扩张、细支气管扩张、支气管壁和血管壁增厚及不规则等征象，12例临床疑诊为间质性肺炎的患者，经胸部hrct诊断的12例，而胸部平片诊断的仅有2例，二者比较差异有显著性（p.05），hrct疑诊的iip患者，经临床激素及免疫抑制剂治疗，病情均得到控制或缓解。结论 hrct表现老年间质性肺炎的特点明显高于胸部平片，且hrct可以反映间质性肺炎的疗效。

Methods by using a broad pH range column(Gemini NX C18,4.6mm×150mm,5μm),basic mobile phase which was[H_2O-ammonia-glacial acetic acid] (96:3.6:0.4)-methanol,the related substances in etimicinsulfate were separated and detected by electrospray ionization and positive ion monitoring,the ESI ion source condition was following: temperature is 350℃,nebulizing pressure is 50 psi,dry gas flow is 10 L /min.some related substances in etimicinsulfate were identified by comparing the retention time in chromatography,~+ spectrum and MS~2 spectrum with reference substances\',the others which haven\'t reference substances were identified or speculated by analyzing their MS~2 fragmentation with the help of a rule summarized from the MS~2 fragmentation of gentamicin C1a,micronomicin and etimicin.Results nineteen related substances in etimicinsulfate were separated and detected.

方法利用宽pH范围的Gemini NX C18(4.6mm×150mm，5μm)色谱柱，在碱性条件下，以[水-氨水-冰醋酸(96：3.6：0.4)]-甲醇(70：30)为流动相，直接分离各组分；并用HPLC-ESI-MS~2检测样品中各组分，质谱条件：ESI离子源，离子源温度350□，雾化室压力50 psi，干燥气流速10 L/min，正离子检测方式；对有对照品的有关物质采用比对有关物质与对照品的色谱保留时间、准分子离子的质荷比及二级质谱方法进行鉴定，无对照品的有关物质，则是先通过解析庆大霉素Cla、小诺霉素、依替米星的二级质谱，对该类化合物的二级质谱裂解规律进行归纳，并以此推测未知新化合物进行结构，再用推测的结构对二级质谱进行解析，如所有的碎片离子均合理解释，表明推测合理。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后，同A组相比：E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高，在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著，在卵泡、黄体中阳性产物表达均较A组显著降低，在子宫中表达较A组变化不明显；同B组相比：E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高，在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低；同C组相比：E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高，在室周核、黄体中均显著降低；同D组相比：E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高，在视上核、弓状核显著降低，在其它部位表达变化差异不显著。

Firstly, according to the relationship between classic Carnot cycle and Lorenz cycle, the paper evaluates the cycle characteristics and thermodynamic properties of NARMS (Non-azeotropic mixtures), and compares the energy-saving difference between pure and mixed refrigerants. The main of factors that affect heat pump operating efficiency are analyzed in theory and using the Second Law of Thermodynamics the exergy are evaluated in components of heat pump system, in the end a conclusion is drew that all of the exergy have bearing on working fluids' properties.

本文首先从经典的Carnot逆循环与Lorenz逆循环相对关系出发，评估了非共沸混合工质循环的特点以及热力学性质；在不同环境条件下，比较了单工质与混合工质的循环特性差异；针对非共沸混合工质热泵循环效率的主要影响因素（即工质本身的热物性和系统部件特性）进行了理论分析，并利用热力学第二定律对热泵系统的各个主要部件进行了可用能分析，最终发现每一处可用能损失均与工质物性有关；在此基础上提出了减少可用能损失的方法—寻找适宜的非共沸混合工质。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷