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Compared with the traditional method, this new method has some advantages:①centrifugation is not necessary, therefore, separation of high molecular weigh DNA is possible;②phenol, chloroform and other toxic organics are not needed;③separation is more efficient and less timeconsuming④the quality of DNA isolated in this way is very high.

当样品裂解液与杂交液1:1混合并用磁性微粒进行小麦黄化苗和种胚的DNA分离与纯化时,效果非常理想。与传统的纯化方法相比,该法具有①快速、简便;②无有机试剂污染;③无需离心分离;④得率和纯度均较高等优点。

They are affected by total aluminiumconcentration and preparing methods as well as basicity mainly in differentcomponents of Ala, Alb and Alc. In addition, the aluminium species component isalso affected by some environmental conditions, such as diluting in water,different pH value, aging time and so on.

此外,一些环境因素也会影响其聚合形态组成的变化,如聚合氯化铝在水中稀释时,不同的稀释阶段和稀释倍数对其各聚合形态组成之间的转化作用不同;随着pH 值的逐渐升高或熟化时间的延长,聚合氯化铝中各种聚合形态均向着聚合度较高的形态转化。

R peak of BNT-BKT ceramics doped with Nd_2O_3 became wider than the basic ceramics without Nd_2O_3. All the ceramics are relaxor ferroelectrics proved by Curie-Weiss law measured at 10 kHz. Otherwise, d_(33) and K_p took on increasing trend and then decreasing trend.

Nd_2O_3掺杂后的陶瓷介电常数整体趋势随Nd_2O_3掺杂量的增高而降低,介电峰呈现更加宽化的趋势,并且从10kHz下的居里-外斯关系图得出在研究范围内所有陶瓷样品均为驰豫铁电体;随Nd_2O_3含量的增加,d_(33)和K_p呈先升高后下降的趋势。

The sequences analysis indicated that complete open read frame of F gene of 3 isolates was 1662bp and encoded F protein of SS3 amino acids/There were 6 identically potential asparagine-linked glycosylation sites and the deduced amino acid sequence of the cleavage site region of F protein of the 3 NDV isolates that had the same amino acids pattern as velogenic strain was ~(112)R-R-Q-K/R-R-F~(117), which was accordant with the results of mean death time of chicken embryos and intracerebral pathogenicity index flCPI.

序列分析结果表明,所获得的3株分离株F基因完整的开放阅读框长度为1662bp,共编码F蛋白的553个氨基酸:QH3、HU和A4株均有相同的6个潜在的糖基化位点,其F基因的裂解位点为~(112)R-R-Q-K/R-R-F~(117),符合强毒株裂解区氨基酸组成的特征,且与MDT和ICPI的测定结果相吻合。

The protective effect SAB was likely not related with the expression of grp75. It showed SAB can decreased the Ca(superscript 2+) level of the cells under glucose deprivation.

免疫细胞化学法和RT-PCR结果均显示丹酚酸对grp75表达没有影响;但经SAB作用后,细胞内Ca(上标 2+)浓度明显降低。

Western blot was used to examine the phosphorylation of NR2A in rat hippocampus. Results Both Src and Fyn antisense ODNs could decrease the phosphorylation of NR2A induced by cerebral ischemia/reperfusion, while administration of Src antisense ODNs had more suppressive effect on the phosphorylation of NR2A than Fyn antisense ODNs.

对NR2亚基有磷酸化调节作用的是Src家族酪氨酸蛋白激酶(Src family of protein tyrosine kinase, Src PTKs),这是一类膜结合的非受体酪氨酸蛋白激酶;现已知Src家族成员中Src、Fyn、Yes、Lyn、Lck在中枢神经系统中均有表达。

In grain-filling stage, the qP values of evolutionary wheats decreased under water stress, while Shan253 and Changwu134 hadn't decreased, indicated that the effect of water stress on the re-oxidation in Q-A of modern wheat was slight, while before this stage, the electron transport from PSⅡto QA restrained by water stress was main factor of photosynthesis non-stomata limitation to modern wheat; The qNP values of hexaploid were higher than diploid and tetraploid wheat, indicated that the ability of using light energy

随小麦由二倍体向四倍体、六倍体及现代小麦进化,表观电子传递速率有增大趋势,其受水分胁迫影响的程度在花前期有加剧趋势,花前期栽培一粒、栽培二粒、普通小麦、陕253和长武134这五种小麦在水分胁迫下表观量子产额的降幅分别为5%、7.47%、14.29%、33.3%和53.49%,光饱和Pn的降幅分别为8.8%、26.14%、23.25%、44.22%和51.29%,光饱和点整体上也是下降的,且这三个指标的降幅随进化程度提高有增大趋势,表明小麦在进化过程中能量转换系统受水分胁迫影响的程度加剧;而在水分胁迫下,小麦的羧化效率和RuBP再生速率也均下降,下降的程

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推荐网络例句

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说,如果是/否的问题回答f可看的价值观念在许多最有限坐标,然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明:基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小,它既能去除奇异点,又能保持图像中目标的边缘不变性,具有很好的滤波效果。

It explains in detail how the methods of elicitation, discussion, and induction are to be used in the physics teaching.

本文根据心理学原理论述了改革初中物理教学的必要性,并对"启发、讨论、归纳法"在物理教学中的应用作了详细的阐述。