地方

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Currently,in the organization and guidance of urbanization,local governments have noticeable advantages in political and economic resources, but also confront with the restriction of some unfavorable factors in the structure of political,economic,cultural,social and administerial systems.

当前我国地方政府在组织领导地方城镇化的进程中，既有可资凭借的显著的政治资源优势，经济体制优势，同时也面临着政治、经济、文化、社会与行政体制结构中的不利因素的制约。

It is to help the local know the deeper meaning of space well through the deep record and description of township. Also, it is to use it as an identification and adscription to the local, and to look for a local cultural perspective on future development and imagination.

透过对聚落做深度的纪录与描述，帮助地方掌握空间的深层意义，并以此作为地方的认同与归属，在未来的发展与想像上能寻求一种在地性的文化视角。

Those scattered around villages also take the part of social life center and thus form a significient position in agrestic settlement.

散布于村村落落的道教建筑还是地方文化活动中心，在地方聚落形态中，居于重要的地位。

Those scattered around villages not only the place in sacrifice Guanyu but also the center of take local cultrual life and thus form a significient position in agrestic settlement.

而散布于众多村落的关帝庙建筑不仅是祭祀关羽场所，还是地方文化生活的活动中心，且在地方聚落形态中，居于非常重要的地位。

Deconstruction is the reflection in the aporia, which focuses on the impossibility, and ＂the passable＂ that can not be reached.

解构就是在绝境中的思考，解构思考不可能性，解构不能去&能去的地方&，去能去的地方那不是解构，不是真正对责任的承担。

Move the stack to a different place in your bag, now take however many of the soulbound/stacked items you want and put the stack of the original item in slot 1 in your bag. For example you've duped 240 arcanite rods, if you want to have 240 warglaives you would put 240 arcanite rods in that spot. Usually 2 would be fine unless it's an item like potions.

移动到另一个地方的叠加在你的袋子，现在却为数众多的物品，你想要/堆放绑定，把堆叠的原始物品放进书包。1槽例如你骗240 arcanite棒，如果你想要有240 warglaives你将会在这个地方arcanite 240杆。2将会是一个多么美好的，除非它是一项像药水。

A leading member of the ruling United Socialist Party, Aristóbulo Istúriz, called for the dismantling of local government, which Mr Chávez wants to replace with communes.

执政的团结社会党领导成员之一Aristóbulo Istúriz要求拆解地方政府，Hugo Chávez想要以公社代替地方政府。

Is the sun able to rise in the place falling behind, and fall behind in assurgent place?

太阳会在落下的地方升起，并在升起的地方落下吗？

All the current talk of preventing banks from becoming "too big to fail" may reflect old atomistic thinking, when in fact the least obvious parts of a system may be its greatest source of vulnerability.

目前所以的关于阻止银行变成&太大而不能倒闭&的说法刚好印证了旧的原子论思维，但是事实上一个系统的最显著的地方恰好是最脆弱的地方。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了