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In the preparation of bio-imprinted enzymes, two bio-imprinting approaches are available:(1) bio-imprinting of egg albumin using heat-denaturalization; and (2) bio-imprinting of egg albumin using bovine serum albumin using dissolving-precipitation process.

在制备生物印迹酶方面,利用蛋白质在水溶液中的柔性,分别采用两种方法对蛋白质进行生物印迹:以热变性法对蛋清蛋白进行生物印迹,以及用溶解沉淀法对牛血清蛋白进行生物印迹。

Based on the "Structural Holes" theory and the concept of "Bridge",combined with the characteristics of the sharing, divide the "Bridge" into five types. Discuss the role of the five types in sharing; Based on the results of the UCINET analysis, express the coordination role of coordinator, and give a optimization way of the inside network; Divide the broker into two types, and make a description of the two types' function in details; Integrate the discussion of gate keeper and representative, and descript the different function of them on the same system; Descript the function of the Simmel model,and illustrate the bad effect of the model and give measures to avoid that.

在简述"结构洞"理论和"桥"的概念的基础上,结合科技资源共享的特点,把"桥"划分为5种类型;对不同类型"桥"在共享中的作用进行论述;基于UCINET分析结果说明协调者的协调作用,并提出内部人际网络优化方法;对中介者进行再次分类,并详述两种模式在科技资源共享中的功能实现;将守门人与发言人综合论述,描述在同一个体系下实现两者不同作用;对齐美尔连带的作用进行论述,对其可能产生的负面影响进行说明并给出相应的避免措施。

At present under the socialist market economy condition, completes the enterprise staff thought political work the key is the Central Party Committee 17 big spirits in every sense of the term implementations basic unit Since the specially reform and open policy, has had many head of undertaking, deceives both superiors and subordinates works for one's own interests under the guise of working for the public, thought the present does not have the movement, in the waist the animal pen axe, the fan which in the hand takes, often on greatly sends the arms, teaches whips the staff, often on to the authority coerces the staff, simply not staff when the person looked, the wages casually decides, bully softly, is hard feared, pays out the wages not to have the pay list, wants to give how many to give how many, Sunday does not let the staff rest, does not pay adds the spot to spend If you offer an opinion to him, regards you is the thorn in one's side, the thorn in the side, does everything possible to carry on retaliates you, and direct descendant wilfully tolerates using the hand in authority to oneself, to give the green light to something, to holds the different political view greatly to open killing They use in the hand two "magic weapon" to benefit as the third party in a dispute, deceive both superiors and subordinates flatter the higher authority, exploits power, violates the country head of undertaking the cadre relative about to forbid in this enterprise to be engaged in production management the stipulation Also uses in the hand the authority private full center pouch, is greedy, does everything possible to concoct pretexts makes the forged account, high keeps off the office supplies with the government money purchase modern age to converge the oneself to have, makes the forged account to reimburse the beach person cost, digs national the corner of a wall Then receives using the duty gives the sales commission,包产 outside the package exits the enterprise outside, fishes for the wealth, even allies the annual bonus, violates the country and the enterprise stipulated, then 参股using the duty manages the enterprise But greatly gives the discount in the staff benefit treatment aspect, in 工资分 is joined to greatly makes the issue, drills the national policy spatial, borrows the wage reform is the excuse, seeks the benefit for own clique, borrows the work then to provide the special sedan car for the enterprise basic unit leadership, all expenses completely are responsible by the unit, to become not to pay the solid private sedan car The party and the country stressed repeatedly that, strictly prohibits the leading cadre to exploit power, but such stipulation into head of undertaking cadre's neglected advice, really it may be said in has the policy, gets down has the countermeasure If the country does not carry on effective supervising and managing to the enterprise, does not carry on the strengthened management, gets down for a long time, they can depend on right and not the improper income become the millionaires and surely the wealthy man, the state asset can massively drain, but government's policy can become a mere scrap of paper, loses it in common people's prestige Let these clothes eating insects embezzle national the property, thus causes the enterprise to move towards goes out of business, such example is too numerous to cite individually The working class is the national master, such formulation until now is does not have the question, but present working class's actual condition how?

目前在社会主义市场经济条件下,做好企业职工思想政治工作的关键是把党中央十七大精神不折不扣的贯彻到基层。特别是改革开放以来,有不少的企业领导,欺上瞒下假公济私,认为现在没有运动,腰里苄着斧子,手里拿的扇子,动不动就大发干戈,训策职工,动不动就以权力要挟职工,简直不把职工当人看,工资随便定,欺出软,硬出怕,发工资没有工资单,想给多少给多少,星期天不让职工休息,不付加斑费。如果你给他提意见,就视你为眼中钉,肉中刺,想方设法进行报复你,利用手中的权力对自己和嫡系任意纵容,大开绿灯,对持不同政见者大开杀界。他们用手中的两件"法宝"渔翁得利,欺上瞒下讨好上级,以权谋私,违反国家关于企业领导干部亲属禁止在本企业从事生产经营活动的规定。还利用手中的权力私饱中囊,多吃多占,想方设法巧立名目做假帐,用公款购买现代高挡办公用品归为己有,做假帐报销滩人成本,挖国家的墙角。利用职务之便收授回扣,把企业外包产品外包出去,从中捞取钱财,甚至加盟人股,违反国家和企业规定,利用职务之便参股办企业。而在职工福利待遇方面大打折扣,在工资分配上大做文章,钻国家政策的空,借工资改革为借口,为自己的小集团谋利益,借工作之便为企业基层的领导配备专门的小轿车,一切费用全部由单位负责,成为名不付实的私家小轿车。党和国家一再强调,严禁领导干部以权谋私,而这样的规定成为企业领导干部的耳旁风,真可谓上上有政策,下有对策。如果国家对企业不进行有效监管,不进行强化管理,长期下去,他们会靠正当和不正当的收入成为百万富翁和千万富翁,国有资产就会大量流失,而政府的政策就会成为一纸空文,失去其在老百姓中的信誉。让那些蛀虫侵吞国家的财产,从而使企业走向倒闭,这样的例子不胜枚举。工人阶级是国家的主人,这样的提法至今是没有问题的,可是现在的工人阶级的实际状况又如何呢?

The contribution to the interruptive coordinator is that if the knowledge discovery can not go on solely in the knowledge base, the process is interrupted and turns to the database; and that if the data mining in the database discovers some redundant attributes, the process is interrupted and turns to the knowledge base to delete the redundant attributes.

对中断型协调器的发展是:原本单纯在知识库中的知识发现,若不能继续进行则要中断发现过程而借助于数据库进行规则的发掘;原本在数据库中的数据挖掘当发现属性的冗余时可以中断挖掘过程而在知识库中删除冗余的属性。

In order to overcome the key problems in the experimental study on microwave plasma thruster, of which the whole system operates under vacuum condition, and to remove the unnecessary energy loss, the microwave source of plasma thruster operated under atmosphere and vacuum condition was developed by applying the design technology of the nonlinear power supply, the integration of attenuator and detector, liquid cooling, after overcoming the gas breakdown problems of electro and microwave apparatus.

为解决微波等离子推力器全系统在真空中进行实验,减少不必要的能量损失,利用开关电源、衰减器和检波器一体化设计技术和液体冷却技术,通过解决微波电子器件在真空中的放电问题,研制出可在大气与真空环境中工作的体积小和质量轻的微波等离子推力器微波源,其输出和反射微波功率可根据磁控管的阳极电流和检波器的输出电压进行测量。

On the basis of ESTs, three antenna specific protein—Ac-ASP1, Ac-ASP2 and Ac-ASP3, were cloned and identified through the transcriptional profiles and immunocytochemical localization in antennal sensilla. All above will produce an important basis to the exquisite olfaction and action of discrimination against others and resistance to mites of A.

在此基础上,着重通过对中蜂主要的触角特异蛋白——Ac-ASP1,Ac-ASP2和Ac-ASP3进行基因克隆,并通过其在触角中的转录表达谱和在触角不同感器内的亚细胞定位,以期对上述分子进行鉴定,最终对揭示中蜂灵敏的嗅觉以及独特的排异和抗螨能力奠定重要的基础。

In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本,提高生产效率,本文针对发酵法生产PHB过程中存在的,发酵液难以分离沉淀,非有机溶剂提取PHB时分子量严重降低等问题,进行了发酵液的絮凝,及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究,同时,对于研究过程中发现的,加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

The propose of the research is to insist on the view of applying to solve the various confusion in applying at present when present The Doctrine of Equivalents in China by analyzing dispute in applying of DOE; apply the research method of history analysis and compare synthetically, integrate with the case analysis and draw the conclusion: It is necessary to induct The Doctrine of Equivalents to prevent the tortious people filching the benefit of the inventor and confirm whether the infringed people imitated and copied patent on purpose, it is international tidal current to apply DOE in the processing of patent infringement cognizance, and firstly it must ensure the protecting confine by explaining the claim with compromise explain method, the difference of different extent in patent creation also effect the demarcation of equivalent extension; it should apply the rule of comparing factor one by one rather than comparing the whole equivalent in comparison of equivalents, and needs to be confirmed by increasing the assistant means in the processing of patent infringement cognizance by using Triple Identity Test of Graver; contrast and analyze whether they are equivalent depend on the level of the common technicians in relative domain and it is scientific and reasonable to confirm equivalent time standard by infringement date; in judicial practice of equivalent cognizance, it may use the experiences in Japan for reference to solve the cognizance misunderstanding area of reality in the law and the technology; The superabundance designation that may be understood as a display unfair contract because of neglect and materially misunderstanding should be conditionally applied in current time.

研究目的在于通过分析等同原则在适用上的争议,提出我国适用等同原则时应坚持适用的观点,解决目前在适用中的各种混乱局面;运用历史分析和综合比较的研究方法,结合案例分析;专利侵权认定中引入等同原则是必要的,其实质是防止侵权人窃取发明人的利益,被侵权人是否故意模仿或抄袭专利,不影响等同侵权的判定,运用等同原则判定专利侵权已成为世界性的潮流;适用等同原则首先要对权利要求进行解释以确定保护范围,在解释权利要求时应采取折衷的解释方法,不同专利创造性程度的差异也影响着等同范围的划定;在等同物的比较上,应当适用&逐个要素比较&,而不能适用&整体比较&,在适用&三一致&标准进行侵权认定时,还需要增加辅助手段予以确定;是否构成等同,应当以所属领域普通技术人员的水平来对比分析,以侵权日为等同的时间标准是科学合理的;在等同认定的司法实践中,解决法律事实和技术事实认定上的误区,可以借鉴日本的经验;多余指定可以理解为一个因疏忽大意或重大误解而订立的显失公平的合同,应当在现阶段有条件限制地予以适用。

First, the paper analyzes 217 factory"s exterior environments completely and objectively by the models of PEST and Boter "s "five forces" and it points out the opportunities and the challenges for 217; Second, making use of the strategic groups theories, the BOSTON matrix analysis, the core ability theories and value chains theories, the paper analyzes the competition position of 217 factory in AC profession. It explicates the main rivals of 217 and the present conditions that 217 factory makes use of chances and averts the exterior threat; The third, according to the exterior environment and internal resources-ability of analysis, the paper makes use of the theories of SWOT to map out competition strategic projects. Immediately after, the paper evaluates and analyzes these competition strategic projects by means of strategy choosing theories.and then it chooses strategic projects of competition under current circumstance; The end, this paper puts forward to some ways in order to carry out these strategic projects of competition that has been chosen.

首先,借助于PEST和波特&五力&模型对217厂所处的外部环境进行了比较全面、客观地分析,指出了217厂所面临的机遇与挑战:其次,运用战略集团群理论、BOSTON矩阵分析、核心能力理论和价值链分析理论对217厂在行业中的竞争地位进行了研究,明确了217厂在行业中所面对的主要竞争对手以及217厂利用外部机会和避开外部威胁的资源和能力情况;第三,根据外部环境分析以及企业内部资源和能力的分析,利用SWOT理论对217厂的竞争战略方案进行初步制定,同时,采用战略选择理论对这些方案进行了评价和分析,选择出了当前情况下217厂的竞争战略方案;最后,围绕所选择的竞争战略方案,提出了实施这些战略的建议以及着眼于企业长远发展的核心竞争力的培育问题。

In demonstration analysis, Hair'double powers washer', the exploitation case is analyzed, and its management pattern in application is tested.

在实证分析中,以海尔&双动力洗衣机&研发案例进行评析,对该管理模式在企业中的运用进行验证。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。