在进行中

The contribution to the interruptive coordinator is that if the knowledge discovery can not go on solely in the knowledge base, the process is interrupted and turns to the database; and that if the data mining in the database discovers some redundant attributes, the process is interrupted and turns to the knowledge base to delete the redundant attributes.

对中断型协调器的发展是：原本单纯在知识库中的知识发现，若不能继续进行则要中断发现过程而借助于数据库进行规则的发掘；原本在数据库中的数据挖掘当发现属性的冗余时可以中断挖掘过程而在知识库中删除冗余的属性。

In order to overcome the key problems in the experimental study on microwave plasma thruster, of which the whole system operates under vacuum condition, and to remove the unnecessary energy loss, the microwave source of plasma thruster operated under atmosphere and vacuum condition was developed by applying the design technology of the nonlinear power supply, the integration of attenuator and detector, liquid cooling, after overcoming the gas breakdown problems of electro and microwave apparatus.

为解决微波等离子推力器全系统在真空中进行实验，减少不必要的能量损失，利用开关电源、衰减器和检波器一体化设计技术和液体冷却技术，通过解决微波电子器件在真空中的放电问题，研制出可在大气与真空环境中工作的体积小和质量轻的微波等离子推力器微波源，其输出和反射微波功率可根据磁控管的阳极电流和检波器的输出电压进行测量。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Recent years as development of the computer and software, it becomes reality using numeric simulation to research grouting in clay. Using the software of PFC2D, the grouting in clay is lucubrated and such achievements are reached: Grouting technologies in existence grouting material and serosity are studied and rational methods are put forward in choosing grouting technology and serosity.(2) Base on numeric simulation biax test a method is explored to simulate clay with PFC2D.(3) A method is explored to simulate grouting using PFC2D.(4) The relation between serosity pressure and diffuseness of grouting in clay with different initial stress is studied.(5) The stress transformation of clay is studied during grouting.(6) The stress distribution is studied under different serosity pressure.(7) The normal stresss and shear stresss at any section are studied.

近年来随着计算技术的发展，使得采用数值模拟的方式研究均质土体中的注浆成为可能，本文则利用离散元程序PFC2D对均质土体中注浆进行了研究，主要做了以下几方面的工作：(1)研究了现有注浆加固技术、注浆材料以及浆液的性质，阐述了合理选择注浆方式以及浆液的方法；(2)采用数值双轴实验，探索了应用离散元模型模拟土体的方法：(3)探索了采用颗粒流程序进行注浆数值模拟的方法途径；(4)研究了在粘土中注浆不同侧向土压力条件下浆液扩散范围与注浆压力规律；(5)研究了注浆过程中不同阶段土体中压应力的变化；(6)研究了不同注浆压力对土体应力分稚的影响：(7)研究了注浆过程中土体中任一截面处正应力与剪应力的对比关系。

At first in the preliminary design this paper computes the linear damping coefficient of the viscous damper employed in this project using Composite Response Spectra approach, meantime, this paper proves that reducing the exponent of velocity of the piston can increase the magnitude of the dissipative energy, because of the viscous damper employed in the practical project character nonlinear behavior; next this chapter performs analysis and comparison between the building without and with the viscous dampers aforementioned when the building suffers from various Time Domain Response, from the angle of displacement, interstroy drift, velocity, interstory velocity, acceleration, energy, maximum axial force of the dampers and of the column etc., in order to prove that the incorporating the viscous dampers into the buildings has great superiority on enhancing its aseismic performance ; furthermore, this chapter also expatiates on diverse assembly form of the viscous dampers that results in the adverse impact on the internal force of the structural member in this building, on the ground of which this segment bring about the preference of the assembly form of the viscous damper; finally this chapter points out the sphere of application of the analysis and computation using Composite Response Spectra approach in design: it is available in well-proportioned stiffness structures and isn抰 available in the structures that contain weak story. It is recommendable, particularly, to incorporate the fluid viscous dampers into this structure that contain weak story to refrain earthquake and reinforce, such as the Central Hall of Shanghai Exhibition Hall.

首先使用了反应谱分析法设计出欲附设的粘滞阻尼器的线性阻尼系数，由于实际工程所用的粘滞阻尼器具有非线性的特性，故而笔者又证明了降低速度指数对提高粘滞阻尼器的耗能量的优势所在；接着分别从位移、层间位移、速度、层间速度、加速度、能量、最大阻尼出力和柱轴力等不同的角度对结构在附设粘滞阻尼器前后的时程反应进行了分析对比，籍此在实际工程中证明了附设粘滞阻尼器对于提高结构的抗震性能的优越性；再者，笔者还阐述了采用不同的粘滞阻尼器布置方式对结构中构件内力特别是柱轴力和基座竖向反力产生的不利影响，并在此基础上提出了布置方式的优选；最后，指出了反应谱法在设计中的适用范围：对于均匀结构反应谱法能够使用，而对于含有薄弱层的结构反应谱法并不适用；对于类似于上海展览馆中央大厅的含薄弱层的结构尤其值得使用粘滞阻尼器的方法进行抗震加固。

On the basis of ESTs, three antenna specific protein—Ac-ASP1, Ac-ASP2 and Ac-ASP3, were cloned and identified through the transcriptional profiles and immunocytochemical localization in antennal sensilla. All above will produce an important basis to the exquisite olfaction and action of discrimination against others and resistance to mites of A.

在此基础上，着重通过对中蜂主要的触角特异蛋白——Ac-ASP1，Ac-ASP2和Ac-ASP3进行基因克隆，并通过其在触角中的转录表达谱和在触角不同感器内的亚细胞定位，以期对上述分子进行鉴定，最终对揭示中蜂灵敏的嗅觉以及独特的排异和抗螨能力奠定重要的基础。

In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本，提高生产效率，本文针对发酵法生产PHB过程中存在的，发酵液难以分离沉淀，非有机溶剂提取PHB时分子量严重降低等问题，进行了发酵液的絮凝，及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究，同时，对于研究过程中发现的，加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

CNTs were first pre-treated using acid solution (HNO3) to obtain CNTs functionalized with carboxylic groups. Subsequently, the stearyl alcohol was grafted onto CNT with the assistance of dehydrating agent, N,N'-dicyclohexyl-carbodiimide. Excellent dispersion in organic solvents such as acetone, THF, and chloroform was found for the modified CNTs (CNT-C18). In addition, the PBS/CNTs nanocomposites were then prepared through simple melt-blending. Mechanical properties, thermal behavior, conductivity of resultant polymer/CNT composites were investigated. The results show that excellent dispersion of nanotubes in the PBS matrices was achieved.

为了增加多壁奈米碳管在复合材料中的分散性，将碳管表面进行化学修饰，首先将多壁奈米碳管浸入HNO3溶液中，进行酸化，使碳管表面带有羧酸的官能基，然后再加入十八烷醇，在温和的条件下，藉由N，N'-二环己基碳二亚胺(N，N'-dicyclohexyl- carbodiimide，DCC)脱水剂脱水，使碳管和十八烷醇之间产生酯基的化学键结，改质后的碳管可以分散在acetone、THF、chloroform…等有机溶剂中，结果显示成功的利用DCC脱水剂完成碳管的改质。

With the society progress,vehicles are used more and more prevalent,because of it,traffic accidents increase quickly,criminal law regards man who makes severe traffic accidents as crimes.and then punish the man by crimial law.but, actually,identify criminal suspect lying on evidences that someone has destroyed individul properties and made accidents which are significant for criminating and measuring crimes.whilst,when progressing in investigating judicial practices of criminal suspects,there are lots of same criminal conditions.for this , should be identifed clearly.

随着社会的发展，交通工具的使用越来越普及，随之而来的是交通事故大幅度增长。刑法对严重的交通肇事行为确认为犯罪，对行为人进行刑事处罚。但是在现实中，对交通肇事涉嫌犯罪的初步认定依赖于对人员伤亡、财产损失以及事故责任的认定。而这些因素对犯罪嫌疑人的定罪与量刑具有决定性的意义。同时，在对交通肇事进行刑事追究的司法实践中，存在诸多与交通肇事罪发生竞合的情形，对此也应进行明晰。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。