在试验中

In this study, the classification of Chinese Babesia spp. infective to cattle were carried out by the classical methods and molecular biotechnology, and investigated the vector ticks of an unidentified Babesia sp. isolated from Xinjiang province by the experimental transmission, and observed the developmental forms of Babesia sp. in the engorged Hyalomma anatolicum anatolicum females;We developed the indirect hemoagglutination test for Babesia bigemina, and evaluated its effect in the sero-survey;We made slow-released injection of imidocarb, and estimated its efficacy on the treatment and prevention of Babesia bigemina infection by the laboratory work and the field tests. The major results were presented as following:1. Discovery of a bovine Babesia sp.

本研究以牛的巴贝斯虫为研究对象，采用传统技术与分子生物学技术相结合的方法对在我国已分离的牛巴贝斯虫病主要病原进行了分类学研究，在实验室内运用蜱传播试验对分离于我国新疆的牛的巴贝斯虫未定种的媒介蜱及其传播方式进行了详细的研究，采用组织抹片方法对牛的巴贝斯虫未定种在小亚璃眼蜱饱血雌虫体内的发育过程进行了观察；建立了间接血球凝集试验诊断方法，并对其在血清学调查中的应用效果进行了考察；研制了咪唑苯脲缓释注射剂，并通过实验室工作和田间试验对防治效果进行了评价。

To breed grafting watermelon Zhuang Miao, terminal of promotion of farming ability of terminal of farming ability promotion and peaceful wave city is in county of peaceful 2005 sea jointly town of street of Ning Hai's head of a county undertook inside the grow seedlings canopy of Shao Xingyong of village of palace of empress or imperial concubine of the first rank " in mother " the card is compressed model matrix nutrition earthen bowl Ci Xi, summarize test result as follows now. Earthen bowl of nutrition of the compression that 1 experiment process suits grafting watermelon grow seedlings to use matrix has 6cm × 2.5cm or 5cm × 2.2cm, what this we choose is 6cm × 2.5cm, smooth squeezing ramming should make seedbed copy first when grow seedlings, seedbed upper berth lies between water to use deep plastic film, in order to prevent moisture leakage and root system next plunging into, make sure nutrient earthen bowl is even and bibulous, filmy all around left and right sides of tall 6cm of bed a low bank of earth between fields, avoid prediction of a person's luck in a given year of moisture side direction.

为了培育嫁接西瓜壮苗，2005年宁海县农技推广总站和宁波市农技推广总站共同在宁海县长街镇娘娘宫村邵兴永的育苗棚内进行了"中慈"牌压缩型基质营养钵(慈溪市中慈生态肥料有限公司生产)和常规塑料钵育苗对嫁接西瓜秧苗素质影响的对比试验，现将试验结果总结如下。1试验过程适合嫁接西瓜育苗用的压缩型基质营养钵有6cm×2.5cm或5cm×2.2cm，这次我们选用的是6cm×2.5cm，育苗时要先将苗床底整平压实，苗床上铺隔水用厚塑料薄膜，以防止水分渗漏和根系下扎，保证营养钵均匀吸水，薄膜四面床埂高6cm左右，防止水分侧向流失。

In order to understand the mechanism of anti-inflammatory effect of PA and carvacrol, the superoxide dismutase, glutahoine reductase, glutahoine peroxidase activities in liver and malondialdehyde , cyclooxygenase 2 (COX-2), tumor necrosis factor-α levels were eveluated. Also observed inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) expression and the pathological histology in λ-carrageenan induced paw edema in mice.

本研究首先以醋酸扭体试验评估左手香水抽提物及其活性成分香芹酚是否具有镇痛效果，以福马林舔足试验辨别其镇痛作用是在周边或是中枢，再以λ-角叉菜胶诱导小鼠足跖肿胀试验探讨其抗发炎作用，并分析其肝脏组织中的超氧歧化酶、麸胱甘肽还原酶、麸胱甘肽过氧化酶活性、发炎足跖组织中的脂质过氧化指标产物丙二醛、肿瘤坏死因子-α及环氧化酶-2之含量变化，观察诱导型一氧化氮合成酶及环氧化酶-2之蛋白质表现量，并将肿胀足跖做病理切片观察之，以探讨左手香水抽提物及香芹酚之抗发炎机转。

To accelerate the widespread of Synchronous Chip Seal and improve the operation quality of Synchronous Chipsealer, key techniques which influence the operation quality is studied detailedly, including the control of distributing quality, the dynamics characteristics of hydraulic system and the spray characteristics of asphalt nozzle of Synchronous Chipsealer.1 The factors which influence Synchronous Chip Seal quality are studied. Fiducial probability of simultaneous distribution density is advised to be used in evaluating the distribution bias and standard deviation of asphalt and aggregates simultaneously, which is more advanced than the single index evaluation method used before.2 The volume efficiency formula of asphalt pump is revised after analyzing the data of volume efficiency with mathematical statistics, which is helpful to match the asphalt spray system and control the flow out of asphalt pump accurately. With equation deduction and experiment, dynamic of hydraulic system used in Synchronous Chipsealer is studied, which lay the theory foundation on improving the stability and efficiency from design and controlling.4 Simulation on hydraulic system used in Synchronous Chipsealer is done with AMESim; the results indicate that multi-circle parallel connected hydraulic system with constant pressure power is better than that with load-sensing pressure power at present, because the latter will oscillate when the multi circles work at the same time. This conclusion has been proved during the debug of Synchronous Chipsealer.5 Asphalt nozzle characteristics, such as flat jet shape, flow distribution, are studied with experiment, the results indicate that cross quality of asphalt distributing is not increased linearly follow the increase of fan overlap level, the best point usually exists between two whole overlap level, asphalt distributing quality is better than±4% at the 2.5 overlap level, meets the superior specified in standard; The distribution quality of Synchronous Chipsealer can be increased by spray asphalt of different flow rang with nozzle of different diameter

为了加快同步碎石封层技术的推广，提高同步碎石封层设备的作业质量，本文对同步碎石封层设备作业质量的影响因素与控制方法、液压系统的动力学特性和沥青喷嘴的喷洒特性等关键技术进行了深入细致的研究：1研究了影响同步碎石封层作业质量的因素，提出了采用联合密度分布置信概率对同步碎石封层中沥青洒布量和碎石撒布量的系统偏差和标准差指标进行综合评价的方法，克服了传统评价方法只能对单一指标进行评价的缺陷；2采用数理统计的方法，研究了沥青泵的容积效率特性，修正了沥青泵容积效率公式，为沥青喷洒系统的匹配和沥青泵出口流量的精确控制提供了依据；采用数理方程推导与试验相结合的方法，研究了同步碎石封层设备液压系统的动力学特性，为从设计和控制的角度提高同步碎石封层设备液压系统的稳定性和效率奠定了理论基础；3运用AMESim软件对采用负载敏感控制和恒压控制的两种同步碎石封层设备液压系统进行仿真，结果表明：负载敏感控制的并联液压系统在多回路同时工作时，由于液压泵排量控制参考压力不稳定，容易引发液压系统振荡，恒压控制的并联液压系统是目前技术条件下比较理想的同步碎石封层设备工作装置驱动方案；该结论亦通过同步碎石封层设备装机试验得到了验证。4采用试验的方法对沥青喷嘴的喷洒特性进行了研究，结果表明：沥青洒布的横向精度并不是随着喷洒扇面重叠度的增加线性增加，其最高点通常出现在二个重叠度之间的"半重叠位置"，"2.5重叠"洒布时，沥青的横向洒布精度≤±4%，可以达到国家相关标准中的优级标准；同步碎石封层设备还可以根据不同洒布量情况下的流量需求，采用不同通径的喷嘴进行喷洒，提高沥青洒布精度。

The tests have Simulated the rainstorm state and the order state, and the results indicated the disposal of microorganisms-aquatic plant system is efficient. The optimization removal rate time was ascertained under the static state test, in dynamic test, comparing and analyzing the effect on removal rates of different concentration wastewater. The results showed, without aeration, the removal rate of CODCr, TN was 85.9%,75.1%, respectively, the highest removal rate of TP was 64.6%, and the concentration of DO increased steadily from 2mg/L to 7mg/L during experiment. In dynamic test, the microorganisms-aquatic plant systems have also appeared better effect on freshet, and the removal rate of TN,TP and CODCr, was 64%,69% and 81%, respectively.

利用接种高效微生物菌剂促进填料快速挂膜，并和水生植物联合作用去除水体中的氮磷等污染物，在静态水体下研究达到最佳去除效率的时间，并在连续流试验下对比分析不同浓度下的去除效率，根据静态模拟试验结果表明，在不曝气，自然复氧条件下，CODCr和TN的最终去除率分别为84.3%和71.4%，TP最高去除率为62.9%，DO也从2mg/L稳定的升高到7mg/L左右；在连续流模拟试验结果表明，该系统对雨后入湖的河水中TN、TP及CODcr的去除率分别稳定在近64%，69%和81％以上。

Result:There are problems in the management of transfusion reaction,such as incomplete registration,limited range of samples and insufficient testing percentage of the cases,inconsistence in the conclusion of two testing methods of drugs-domestic:hare testing method and limuloid testing method,unawareness of the medical staff and ineffective investiga...

结果 ：输液反应管理中存在以下问题：登记不全；取样范围局限和热原检测比例不足；两种药物热原检测方法—家兔试验法与鲎试验法的结论有不一致性；医务人员思想认识模糊，原因排查不力。结论：健全医院输液反应管理制度，做好各种原始记录，在输液反应热原检测中，鲎试验法更为可靠及时

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The proposed method contains two main parts: one part is determination of the noncontinuous factors by the orthogonal design method and the other is determination of the continuous factors by the uniform design method.

联合试验设计法主要分为：用正交试验设计法确定非连续性因素及其在备选水平中的适宜水平，以及用均匀试验设计确定连续性因素及其备选水平中适宜的水平范围两部分。

Individuals who have a positive history but a negative skin test tolerate penicillin-class antibiotics as well as they tolerate other antibiotics, according to AAAAI literature. Although the skin test is as safe as skin testing for other allergens, a "very low rate of resensitization" will occur after skin testing and reuse of penicillin. The skin test exposes the patient to an amount of penicillin that consists of 1/5,000th of that in a typical prescription.

根据AAAAI的文献指出，有盘尼西林过敏的病历却在盘尼西林测试中出现阴性反应者，对其他抗生素有著一样的耐受特质；虽然盘尼西林皮肤测试和其他的过敏原皮肤试验具有一样的安全性，盘尼西林在经过皮肤试验及使用过后，再度产生敏感的比率却非常的低；皮肤测试中，患中会暴露在正常盘尼西林处方剂量的5000分之一。

Study on the rubber stator compoundof one step injection screw drilling tool;2. he paper presents a test method to research the dynamic property of the rubber stator for screw pump,compares the result of static and dynamic tests and selects the formulation according to the tests.

提出研究螺杆泵定子橡胶动态性能的试验方法，对静态试验及动态试验结果进行对比，并应用其试验对配方进行筛选，结果表明，橡胶压缩温升试验能较好地表征螺杆泵定子橡胶的动态性能，为橡胶定子的研究提供了较为可靠的依据，并在实际应用中得以证实。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。