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Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

Sum up as follows now. This group uses 1 clinical data ancon vein is pressed determine heart function of the patient 44, male 24, female 20; age 46 years old of ~79 year old, average 56 years old of; classification by function of new York heart, heart failure Ⅲ spends 6,Ⅱ spends 23,Ⅰ spends 15; right heart failure 31, complete heart failure heart disease of sex of source of 13; lung 14, hypertensive heart disease 8, coronary heart disease 5 (among them acute cardiac muscle straightens dead 2), outspread sex cardiac muscle is ill 7, rheumatism sex heart disease 4, hypertensive heart disease is amalgamative coronary heart disease 3, be short of disease of cardiac muscle of courage and uprightness 2, congenital heart disease 1. 2 detect 2.1 equipment treat the method dish 1, l measure press glass to be in charge of 1 (long 30cm, internal diameter 4mm), also can use measure a head to press a canal to replace, 10mL injector 1, 12500U heparin fluid 1. 2.2 operations patient is taken smooth lie, make be exhibited outside the upper arm show ° of 45 °~60 with trunk, and difficulty breathes like the patient in same horizontal; with right atrium, desirable lie partly, arm height is equivalent to the 2nd costal region.

现总结如下。1临床资料本组采用肘静脉压测定病人的心功能44例，男24例，女20例；年龄46岁~79岁，平均56岁；按纽约心功能分级，心力衰竭Ⅲ度6例，Ⅱ度23例，Ⅰ度15例；右心力衰竭31例，全心力衰竭13例；肺源性心脏病14例，高血压心脏病8例，冠心病5例(其中急性心肌梗死2例)，扩张性心肌病7例，风湿性心脏病4例，高血压心脏病合并冠心病3例，缺血性心肌病2例，先天性心脏病1例。2检测方法2.1设备治疗盘1个，L型测压玻璃管1个(长30cm，内径4mm)，也可以用测脑压管代替，10mL注射器1具，12500U肝素液1支。2.2操作病人取平卧位，使上臂外展和躯干呈45°~60°，且和右心房在同一水平面；如病人呼吸困难，可取半卧位，手臂高度相当于第2肋间。

The species does exist. It will be remembered that he pretended to have served in the army; he was in the habit of relating with exuberance, how, being a sergeant in the 6th or the 9th light something or other, at Waterloo, he had alone, and in the presence of a squadron of death-dealing hussars, covered with his body and saved from death, in the midst of the grape-shot,"a general, who had been dangerously wounded."

我们记得他妄称自己有过汗马功劳，他常说得天花乱坠，告诉别人说他在滑铁卢战争时是某个第六或第九轻骑队的中士，他单独抵抗一中队杀人不眨眼的骑兵，用自己的身体遮护过一位&受了重伤的将军&，并且把他从枪林弹雨中救了出来。

Headquartered in Bellevue, WA, MulvannyG2 has close to 400 employees.

MulvannyG2建筑设计公司是美国华盛顿州第三大建筑设计公司，在全美也排名到了第九位。

COFCO Property Office董秘inquiry, a staff member, said third-quarter inventory increase was mainly attributable to May this year to 2.5 billion in Beijing won the bid after the sand block Tongkuangyu Third quarter included in inventory.

中粮地产董秘办公室询问，一位工作人员说，第三季度库存的增加主要是由于今年5月的25亿，在北京赢得了后沙峪第三季度块列入投标库存。

During the past twenty years of Reform and Opening to the outside world, China has been developing fast. The ranking of China's foreign trade amount has grown from the thirty-second in 1978 to the third in 2006, just after American and Germany.

改革开放的20多年里中国经济发展迅速，1978年中国外贸额在世界排名第32位，而到2006年，中国已成为仅次于美国、德国的世界第三大贸易国。

They are the 11th-seeded team in the Eastern Conference, 1 1/2 games out of the playoff spot.

他们在东部的排名是第11位，距离进季后赛的第八名还有1个半胜场的差距。

In the previous year's ranking, the Chinese had the fifth-fastest computer, a system that was based at a National Supercomputing Center in Tianjin, China.

在去年这个时候的排名中，中国天津国家超级计算机中心的一台计算机系统获得第五，这次它落到了第七位。

The total amount a row of the our country fresh water resources sixth in the world, but the person all occupies the quantity to be worth equally for the world only of 1/4, occupies the 109 of world, and the resources of water distributes in the time and region up very unbalanced, have 10 province, cities, the water resources of the autonomous region F through low in at least of existence line, person there all the resources of water owns the quantity shortage 500 sign the square rice.

我国淡水资源总量名列世界第六，但人均占有量仅为世界平均值的1/4，位居世界第109 位，而且水资源在时间和地区分布上很不均衡，有10个省、市、自治区的水资源己经低于起码的生存线，那里的人均水资源拥有量不足500立方米。

One poll ranks him fourth among Fifth Republic presidents, with 60% approval.

一次调查把他排在法兰西第五共和国总统中的第四位，60%的人赞同。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。