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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

In this paper the authors reviewed the characteristics in the different phases in the life cycle of ferns. Special attentions were given to the effect of light, plant growth regulators, water and temperature on the fern gametophyte development, and the effect of antheridiogen, light, calcium and other plant growth regulator on the formation and differentiation of fern sex organ.

作者概述了蕨类植物生活周期中不同发育阶段的特点,并着重介绍了光、植物生长调节物质、水和温度等因素对蕨类植物配子体发育的影响以及成精子囊素、光、钙离子和其它植物生长调节物质在性器官形成和分化过程中的作用。

Although several gametogenesis-specific genes were identified previously, more genes related to the ovarian development are expected to be involved due to the complexity of gametogenesis.

虽然已有一些调控卵子生长和卵泡发育的特异性基因被发现,但与卵巢发育和卵子生长相关的更多基因的功能有待于进一步研究,阐明其在这复杂过程中的具体分子机制。

In our experiment, we found two abnormal phenotypes that one only the last three segments develop into pupa, but the other segments are still keep the larva characters; another sample has differently develop speed between abdomen and backside, which resulted in the back develop into pupa but the abdomen were still the larva characters. When we add Az in the feed of injected EcR-dsRNA samples, in the 13 live larvae we found that 6 have the abnormal proleg phenotypes.

在另一组注射EcR-dsRNA以后通过浸叶法添食不同浓度的印楝素,在10ppm处理组存活的13个个体中有6个出现特异表型,其中两个幼虫为不完全蜕皮,尾部体节无法蜕皮导致发育受阻;另外发现4个供试虫体发育至五龄时出现腹足缺失表型,其中有两个是腹足几乎全部缺失,腹足应该生长的部位只有少数几个突起,没有发育成形的腹足;一个出现左侧腹足完全缺失,右侧第二腹足缺失表型;另一个个体只缺失了左侧最后一个腹足。

The short shoot differentiation came from the lateral buds among the bud scales, in the initial stage, the lateral buds elongating, and then differentiating two "anisomerous"(one was small, the other was big) needle primordia in the elongating courses; the big one differentiating once more, and formed three needle primordia.

短枝的发育来源于长枝上芽鳞间的侧芽,在发育的初期,侧芽进行伸长生长,在伸长生长的过程中首先分化出一大一小&不对称&的两个针叶原基,大的叶原基进一步分化,形成三个针叶原基。

For example, alkaline elements can affect diffusion velocity of Zr, Si and O ions although these alkaline elements cannot exist in crystal structure of zircon. However, K rich media and Na rich media exert different effects on the growth of crystal form: in the K rich media, growth speed of prism faces in the vertical direction is much higher than that of pyramid faces, and {111} growth speed in the vertical direction is higher than {311},which decides the formation of bipyramids dominated by {311} pyramids; in the Na rich media, both prism and pyramid grow to a certain extent, but the former is smaller than the latter.{100} growth speed in the vertical direction is lower than {110}, while {111} growth velocity in the vertical diredtion is lower than that of {311},resulting in the growth of the crystal form characterized by {100} short prism and {111} pyramid.

例如,尽管碱性元素并不进入锆石晶格,但它们可以影响Zr、Si、O离子的扩散速度,但是富钾和富钠的介质对锆石晶形发育的影响是有差别的,表现为:在富钾的介质中,将导致柱面的法向生长速度较锥面快得多,而锥面中,{111}的法向生长又较{311}快,由此决定了形成以{311}锥为主的双锥状晶体;在富钠的介质中,柱面和锥面都有一定程度的发育,但前者弱于后者,而柱面中,{100}的法向生长较{110}慢,由此决定了形成以{100}柱和{111}锥为主的短柱锥状晶体。

The exposed massive cold-seep carbonates provided substrate for the encrustations of corals as well as coralline algae and might have played a crucial role in the initial development of coral reefs in a siliciclastic paleoenvironment of active tectonic setting. This is probable the first case of the world that cold-seep carbonates acting as initial colonization hardgrounds of hermatypic corals and corallines.

在此基底泥岩快速浅化的过程中,出露的块状冷泉碳酸盐岩成为不稳定地体构造背景中,珊瑚与珊瑚藻等表覆生物在矽质碎屑环境生长的硬底质,可能在礁体的最初发育扮演关键性的角色;此种冷泉碳酸盐岩成为造礁珊瑚与珊瑚藻在矽质碎屑古环境中最初生长硬底质的现象,很可能为世界首例。

At 24 h, Ang1 was expressed mainly in the lens, optic cup, mesencephalon, ventral part of hindbrain, and myotomes in the somites, while zfTie2 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk. VEGF was expressed mainly in the lens, optic cup, mesencephalon and myotomes in the somites, while zfFlk1 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk.

结果从受精卵胚胎发育第12小时可见血管内皮细胞生长因子和血管发生素1及其相应受体flk1和Tie2的表达;在受精卵胚胎发育的第16小时,血管内皮细胞生长因子和血管发生素1主要在头部和尾部胚芽表达;第24小时在眼球、视窝、中脑、后脑的腹前侧和肌节中表达,Tie2和Flk1主要在肌间背部动脉血管、躯干新生血管芽和轴向静脉中表达。

In addition, the scanning paragraph of sodium dodecyl sulfate polyacrylamine gel electrophoresis of proteins in root and leaf cell after treatment with Eu〓 was analyzed.

本论文在结合电镜和荧光分析定位以及稀土离子对Ca〓信使系统组分的影响基础上,对Eu〓影响植物生长发育过程中的相关生理调节机制进行了解释。

Mice deficient in CRMP-1 not only showed reduced weight in cerebellum but also lack the normal organic structure in VIb lobe suggested that CRMP-1 also involved in cerebellum development.

另一方面,CRMP-1基因缺乏小鼠小脑发育也受到影响,除了重量较野生型小鼠轻外,小脑十个由颗粒层形成的摺叶中,编号第VIb的摺叶在发育上也有缺损,不明显,推测CRMP-1亦调控小脑的生长与发育。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?