在生物体外

Objectives: To investigate the effects of adhesion and proliferation of bone mesenchymal stem cells in the surface of lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol PLGA- tri-block polymer scaffolds, try to find a new biomaterial to induce seed cells in vitro for bone tissue engineering.

目的：探讨大鼠骨髓间充质干细胞BMSCs在聚丙交酯/乙交酯/天冬氨酸-聚乙二醇三嵌段多元共聚物 PLGA-表面粘附、增殖的情况，为组织工程学体外诱导种子细胞生长提供新的生物材料。

Aim: this article studied the separation condition of vitro gel of Brasenia schrebeVs tender leaves and its basic component, the separation rule of Zinc in various organs and biomacromolecule of Brasenia schrebeVs tender leaves, that laid the groundwork for deep-processing Brasenia schrebei; At the same time, the purification of protein rich in Zinc laid the groundwork for studying its structure, characteristics and the binding organism between Zinc and protein.

目的：研究莼菜嫩叶体外胶质的分离条件和其基本组成以及锌在莼菜各器官和嫩叶生物大分子中分布的规律，为莼菜深加工打下基础；分离纯化富锌蛋白，为研究其结构、性质以及锌与莼菜蛋白质的结合机制打下基础。

Objective1 To observe the biofilm formation on urinary catheters from critical patients.2 To analyse the risk factors of biofilm formation on urinary catheters from critical patients.3 To compare the eradicative effects of different irrigation methods in vitro.

目的1观察重危患者留置尿管表面生物被膜的形成情况。2分析重危患者留置尿管表面BF形成的危险因素。3比较体外不同冲洗法在清除尿管表面BF中的作用。

These transgenic cells were able to secrete hEGF protein to certain extent, and have the biological activities to enhance the growth rate of Hacat cells in vitro. Conclusion: Adult epidermal keratinocytes in vitro transfected with exogenetic hEGF cDNA can express and secrete active hEGF.

质粒pcDNA3.1-hEGF在脂质体介导下成功转染成人皮肤角质形成细胞，转基因细胞能分泌有生物活性的EGF；体外培养的成人皮肤角质形成细胞可见少量EGF分泌。

Lupeol, a pentacyclic triterpene present in mango, is a biologically active compound that has been reported to possess a number of pharmacological properties in the in vivo and in vitro studies.

羽扇豆醇是芒果所含的五环三萜，据报道，羽扇豆醇是一类生物活性物质，在体内及体外研究中均表现出多种药理特性。

Pure merwinite powers were synthesized by sot-gel process, and the column-like merwinite with dimensions of l0mm×5mm was prepared under the 10MPa uniaxial pressure. The in vitro bioactivity of merwinite powers was studied by soaking them into simulated body fluid.

用溶胶－凝胶法制备出纯相镁蔷薇辉石(Ca3MgSi2O8)粉体，并在10MPa的轴向压力下，将其压制成尺寸为10mm×5mm的圆柱状块体，通过模拟体液浸泡对其体外生物活性进行研究。

New antifungal agents have been developed over the past years based on the discovery of many new target sites of Candida albicands. For example, Echinocandins, aiming at the cell wall of Candida albicands, showed strong antifungal activities to fluconazole resistant candidas and fungal biofilm. Moreover, because β-glucan synthase does not exist on the cell membrane of mammalian cells, Echinocandins has a low toxicity and a promising clinical future. Though the mechanism of Histatin is not clear, it has strong activity not only on candida resistant of polyene and azole, but also to Candida parapsilosis, Candida krusei and Cry ptococcus neoformans. Berberine and Ocimum gratissimum L. were also found to have prominent anti-fungi activities.

近年来相继提出了许多抗白念珠菌药物作用的新靶点并成功开发出一些活性强大，疗效确切的抗真菌药，如以真菌细胞壁为靶点开发出棘白菌素类药物卡泊芬净、米卡芬净等，该类药物主要抑制β-葡聚糖合成酶，体外对氟康唑耐药的念珠菌属及真菌生物被膜表现出强大的抗菌活性，又由于β-葡聚糖合成酶在哺乳类动物细胞内不存在，故具有高效低毒的临床效果，是一极具前途的新型抗真菌药；以真菌细胞膜为靶点开发出Histatin类药物，虽然作用机制目前还不完全明了，但其作用不同于临床常用的多烯类和氮唑类，并已经发现Histatin5对两性霉素B和氮唑类耐药的念珠菌有效，并且对非白念珠菌如光滑念珠菌、克柔氏念株菌、隐球菌等也有杀灭作用；通过对植物药物的研究发现黄连素和丁香罗勒Ocimum gratissimum L。

By the polycondensation of amino acid 5-fluorouracil -1, 3-dipropyl esters with bis (2-chloroethyl) phos- phoramidic dichloride in the presence of 〓 or in vacuo, ten new polymers containing 5- fluorouracil, nitrogen mustard and amino acid were synthesized.

将1，3-双-5-氟尿嘧啶与二氯膦甲酸乙酯或二氯膦乙酸乙酯在DMF中反应制备得到20个三元共聚物，体外抗肿瘤试验表明：含有甘氨酸、缬氨酸、苯丙氨酸和酪氨酸的聚合加显示了一定的选择性，并且分析讨论了生物活性与结构之间的关系。

Tissue engineering is a multidisciplinary science merging with cell biology, material science, engineering and chemistry. Its basic idea is to expand a number of cells in vitro and seed them on a polysynthetic biodegradable polymer structure, and finally enable the organization of 3-dimmentional and functional tissues or organs.

组织工程技术是融合了细胞生物学、材料科学、工程学和化学的一门边缘学科，它的基本思想是用少量组织细胞经体外培养扩增后播种在可降解的生物支架材料上，再回植入体内形成有功能的组织或器官。

Ga〓 has similar oxidation state of 3+ chemical valence, so we synthesized 〓Ga-EDTMP (ethylene diamine tetramethylene phosphate) and studied its biodistrbution and radio toxicity of 〓Ga in the human osteosarcoma cell line to evaluate the possibility as a bone pain palliating pharmaceutical.

利用〓Ga〓与〓Sm〓化学性质类似，用EDTMP载带〓Ga〓，研究其在体外对骨肉瘤细胞的作用及小鼠体内的生物分布，探讨〓Ga-EDTMP作为骨转移性骨疼痛姑息治疗药物的可能性。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。