在生物体外

Amnion has good water adsorptivity and biocompatibility with fibrocartilage cells. It can be used as scaffold for tissue engineering of temporomandbular joint.

羊膜表现出良好的吸水性、细胞吸附性和体外生物相容性，髁突细胞可在其上黏附、增殖，羊膜适于作为髁突组织工程支架材料。

Advance basic research has confirmed that the alkaloids extracted from thunberg fritillary bulb which is widely used in clinical medicine in vitro and in animal experiments have reversed MDR of leukemia. It will increase resistance of acute leukemia cells anticancer drug concentration and lower resistance the expression of Pgp. Pre-Clinical Experimental Research also shows that the conventional chemotherapy combined with the powder of thunberg fritillary bulb for the treatment of acute leukemia. Clinical complete response rate was higher, especially for patients with refractory or relapsed leukemia patients better clinical remission rate. In addition, Caladium particles Slices with chemotherapy in the treatment of RAL Clinical studies also show the Chinese refractory leukemia improve clinical efficacy safe and reliable.

先期基础研究已证实：临床常用的化痰散结中药浙贝母提取的生物碱体外及动物实验均有逆转白血病多药耐药的生物活性，能增加耐药的急性白血病细胞内抗癌药物浓度，降低耐药蛋白Pgp的表达；临床预实验研究也表明，常规化疗方案配合浙贝母粉用于急性白血病临床治疗，其临床完全缓解率明显高于对照组，尤其对难治及复发白血病患者临床缓解率更佳；此外，单药浙贝母颗粒配合化疗治疗RAL的临床研究亦显示中药在提高难治性白血病临床疗效方面安全可靠。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

It is concluded that ex vivo expanded bone marrow CD34+CD59+ cells from patients with paroxysmal nocturnal hemoglobinuria could keep their biological characteristics and ability to reconstruct hemopoiesis in irradiated BALB/c mice.www.yixuelw.cn

PNH病人骨髓CD34+CD59+细胞体外扩增后仍能保持造血祖细胞的生物特性，在照射的裸鼠中能重建筑血。

The effects of DHAP on biomechanic properties of cultu red pulmonary vessel endothelial cells and isolated erythrocytes were studied by using micropipette aspiration methods.

采用微管吸吮技术观察了体外培养肺血管内皮细胞及新鲜分离的红细胞在用青心酮处理后生物力学特性的变化。

The study results showed that the bioaccessibilities of p,p'-DDT, p,p'-DDD and p,p'-DDE in intestinal solution were higher than in stomach,and the bile and pancreatin had positive effects to them.

采用基于生理学的体外实验，通过模拟人体胃和小肠的环境，分析了胡萝卜中p，p'-DDT，p，p'-DDD和p，p'-DDE在人体胃肠系统的生物有效性。

In the protein adsorption and osteoblast culture in vitro, more surface hydroxyl groups and higher polar component of surface energy led to more protein and cell adsorbed, and higher cellular activity.

在本论文中，通过金红石二氧化钛膜、锐钛矿二氧化钛膜、含钙的钛表面、含磷的钛表面和Ti-羟基磷灰石涂层的表征，结合羟基磷灰石沉积、蛋白质吸附和细胞体外培养，研究了钛的表面表征与生物活性的关系。

PDLSCs and SCAP grew very well on the scaffold of HA/TCP in vitro. Conclusion:The miniature pig PDLSCs and SCAP, which can be cultured successfully in vitro, may be useful cell sources for the regeneration of periodontal and tooth tissues.

小型猪牙周膜干细胞及根尖牙乳头干细胞可被成功的分离培养，在体外表现出良好的生长状态，有望为牙周组织再生和生物牙根再生提供有用的细胞来源。

To study the biotransformation of deoxyaconitine in intestine, aconitine was incubated with human intestinal bacteria in vitro .

采用人肠内细菌和乌头碱体外温孵的方法，探讨去氧乌头碱在人肠内的生物转化。

To study the biotransformation of 16- O -demethylaconitine in human intestine, aconitine was incubated with human intestinal bacteria in vitro .

采用乌头碱和人肠内细菌体外温孵的方法，探讨乌头碱在肠内的生物转化规律。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。