在生物体外

METHODS Human secondary follicles were isolated from ovarian tissues obtained from cancer patients and grown in vitro within a bio-engineered culture system for 30 days.

方法从癌症患者的人卵巢组织中分离出次级卵泡，并在体外的生物工程培养体系中培养30天。

In this paper,the effect of horseradish peroxidase on the biosynthesis of artemisinin was studied.

通过细胞提取液，在体外条件下研究了辣根过氧化物酶对青蒿素生物合成的影响。

OBJECTIVE: To observe in vitro biocompatibility of rabbit acellular cartilaginous matrix and adipose-derived stem cells.

目的：观察兔软骨基质支架与同种异体脂肪干细胞在体外的生物相容性。

However, the in vitro directional differentiation of BMSCs into neuronal cells is easy affected by various factors.

但是在体外条件下通过化学和生物诱导物使骨髓间充质干细胞转化为神经元，影响骨髓间充质干细胞向神经细胞、胶质细胞系统定向分化的因素比较复杂。

The compounds of CHA with cells were implanted into nude mouse, and the ectopia osteogenesis was detected.

2观察HBMSC与CHA在体外复合培养条件下的生长情况及生物相容性，为骨组织工程选择适宜的种子细胞载体。

All patients all fall in body outer circulation, undertake excrescence keeps clear of reach former hair heart disease correctional operation.

所有患者均在体外循环下，进行赘生物清除及原发心脏病矫正手术。

Mesencephalic neural stem cell biological characteristics, isolation, proliferation and differentiation are subject to further study. In addition, biological safety problems using mesencephalic neural stem cells in the human body gradually attracted the attention of the people.

同时中脑神经干细胞应用于人体治疗的生物安全性问题也逐渐引起人们的重视，如在体外多次传代的神经干细胞是否会发生转化，是否会带来一定的副作用，甚至致瘤性等均在进一步探索中。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

METHODS Recombinant human protein kinase CK2 α and β subunits were cloned and expressed by genetic engineering, and purified to homogeneity. The two subunits were mixed at equal molar ratio and reconstituted CK2 holoenzyme, which exerted the maximum biological activity.

方法利用基因工程克隆，表达和纯化获得重组人蛋白激酶CK2 α和β亚基，在体外等摩尔数混合构成有最大生物活性的重组CK2全酶，在不同条件下测定CK2的活性。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。