在生物体外

Our data support the notion that human follicle development can be achieved in vitro in a bio-engineered culture system.

结论我们的数据表明，人类卵泡在体外生物工程培养体系中是可以发育的，但仍需要更多的研究进一步揭示体外培养卵泡是否获得充分大小的卵母细胞，而且卵母细胞是否有能力恢复减数分裂和受精。

To evaluate the bioactivity of the glass-ceramics,the specimens were soaked in the simulated body fluid. The results indicate that fluorapatite shows needle-like microstructure, which is similar to that in human bone and teeth. The formation of needle-like fluorapatite is related to the heat treatment procedure, which results in needle-like fluorapatite crystals by accelerating one-dimension growth along the c-axis through the screw dislocation growth mechanism. The mechanical properties of the glass-ceramics are close to those of the human bone and teeth. The bioactivity test results show that apatite layer is precipitated on the materials surface after 3 d immersion in SBF, which indicates the glass-ceramics have a good bioactivity.

研究结果表明：氟磷灰石相呈针状，与人体骨和牙齿的磷灰石形貌相似，有利于提高材料的生物活性和生物相容性；氟磷灰石的形成是材料被迅速加热至高温进行热处理，使磷灰石以螺旋位错生长机制沿c轴晶化长大所致；经热处理获得的材料具有优良的力学性能，基本满足骨科和齿科用生物材料的性能要求；材料在体外模拟体液中浸泡3 d后，样品表面已有磷灰石层生成，表明该材料具有较高的生物活性。

It was also studied the tissue distribution characteristics of harmine intravenous emulsion in mice.

采用反向透析技术测定了乳剂的体外释药性能，这种释放模型较好地摸拟了体内生物环境，用多个小透析囊巧妙地实现了药物分子与介质中其它成分的直接分离，使含量测定易于进行；实验结果表明，乳剂粒子内药物向介质的释放过程和释放出来的药物分子渗透进入囊的过程均为被动扩散机制，并且药物在乳剂中的相分配不同其体外释药速度，尤其是最初释药阶段的速度存在明显差异（P.05）。

The analytical approach/tool of MultipleScale Integrated Assessment of Societal Metabolism, proposed by Mario Giampietro,Kozo Mayumi, et al., based on the congruence among resource "Human Activities", resource "Exosomatic Energy Flows", and "Added Value Flows" in the hierarchical socioeconomic systems, using, in an integrated way, incommensurable variables referring to nonequivalent descriptive domains(coming from various scientific disciplines) and data gathered at distinct hierarchical levels, addresses the various relevant dimensions of societal development and sustainability.

社会代谢多尺度综合评估"分析方法，根据"人类活动"资源、"体外能"资源和"增加值三者在社会经济系统中的叠合关系，将各个尺度（如国家、经济部门、次级部门、省/州、社区、直至个别家庭）上，社会经济系统的能量代谢过程，与该系统的经济、人口（如人口的年龄结构和性别构成）、生态和社会（如义务教育和退休年龄的规定）特征结合起来，利用多个关系式，计算出社会经济系统的人类活动量、体外能投入量、劳动生产率、生物—经济压力和体外能超循环强度等参数、指标，进而综合地分析评估社会经济系统发展与可持续性。

Although the primary human hepatocytes would be ideal for bioartificial livers, the shortage of hepatocytes and the difficulty to increase the doubling time of human hepatocytes in vitro limit the clinical application.

肝细胞是生物人工肝支持系统的核心部分，从生物工程学角度讲，理想的用于生物人工肝的肝细胞要人源性、表型正常，容易获得和培养，能在体外迅速生长增殖至高密度，保持良好的分化状态，具有成熟肝细胞的所有生物代谢功能。

Preliminary antitumor activity in vitro of the new compounds indicated that the compounds have obvious inhibitory effect on human leukaemia cell (HL-60) and human galactophore cancer cell (MDA-MB-468) in the concentration of 10-4 mol/L. This result is a basis for further study on the synthesis and biological activity of this kind of compounds.

对所合成的10个新化合物进行了抗肿瘤体外生物活性试验，初步生物活性实验结果表明，该类化合物在浓度为10-4 mol/L时对人白血病细胞株(HL-60)和人乳腺癌细胞株(MDA-MB-468)有明显的抑制作用，在浓度为10-5 mol/L时没有明显的抑制作用，这一研究结果为进一步研究此类化合物的合成、生物活性的研究奠定了基础。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

Bioreactor is a kind of device intended for in vitro tissue culture that incubates the cells or tissues taken from living bodies in simulated physiological environment in vivo. On the basis of original cell culture, the present bioreactors apply biomechanical stimulation to simulate the stressed environment of articular cartilage in vivo.

生物反应器是一种体外的组织培养装置，它将活体内取出的细胞或组织在模拟体内生理环境下进行孵育培养使之生长分化，现有的生物反应器主要是在原有细胞培养的基础上加以力学刺激以模拟关节软骨在体内的应力环境。

The analytical approach/tool of MultipleScale Integrated Assessment of Societal Metabolism, proposed by Mario Giampietro,Kozo Mayumi, et al., based on the congruence among resource "Human Activities", resource "Exosomatic Energy Flows", and "Added Value Flows" in the hierarchical socioeconomic systems, using, in an integrated way, incommensurable variables referring to nonequivalent descriptive domains(coming from various scientific disciplines) and data gathered at distinct hierarchical levels, addresses the various relevant dimensions of societal development and sustainability.

社会代谢多尺度综合评估&分析方法，根据&人类活动&资源、&体外能&资源和&增加值三者在社会经济系统中的叠合关系，将各个尺度（如国家、经济部门、次级部门、省/州、社区、直至个别家庭）上，社会经济系统的能量代谢过程，与该系统的经济、人口（如人口的年龄结构和性别构成）、生态和社会（如义务教育和退休年龄的规定）特征结合起来，利用多个关系式，计算出社会经济系统的人类活动量、体外能投入量、劳动生产率、生物—经济压力和体外能超循环强度等参数、指标，进而综合地分析评估社会经济系统发展与可持续性。

Chapter Three. The Studies on Gene Therapy for Retinal Degeneration of the Mice and It's Mechanism Objective: To evaluate the abilities of adeno-associated virtus carrying the basic fibroblast growth factor gene as a therapy for retinal photoreceptors in slow retinal degerneration mice on the basis of the experiment results as mentioned above. Methods: On the born day of rds mice, IPE cells were isolated with enzyme-assisted microdissection from pigs and were cultured.

第三章 视网膜色素变性小鼠的基因治疗及其机制目的：在体外实验证明AAV载体可以高效安全转染IPE细胞并且可以表达具有生物活性的bFGF的基础上，应用AAV载体携带bFGF基因以先体外，后体内法和体内法两种方式进行视网膜色素变性鼠(retinal degemeration slow，rds)的基因治疗，在分子生物学、形态学和功能学上进行基因治疗的初步观察方法：在rds鼠出生的当天取眼球，采用酶辅助的显微分离方法分离培养IPE细胞。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。