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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

The author creates the AutomaticallyDiscriminational Model for the Classification of Sandstones System, thatis to say, people can complete the vastly sample s classification of sandstonesaccurately and swiftly, meantime, diminish the possibility of mortal mistake. Based on the study of the basic sandstone component before embedding, the typeof authigenic mineral, and the forming mechanism of porosity in Upper Paleozoicreservoirs in Ordos basin, the author selects the reasonable experiment objects forWater-Rock Interaction modeling study, designs the relatively reactant and resultantduring the diagenesis period, builds the solid-liquid thermodynamics model with theprinciple of thermodynamic phase equilibrium for kinds of primary minerals (potashfeldspar, albite, anorthite, and pyroxene, hornblende) which dissolve in acidic media,and forms the corresponding thermodynamics test database.

在对鄂尔多斯盆地上古生界储层砂岩埋藏前组成、自生矿物类型和孔隙构成研究的基础之上,确定合理的沉积盆地水—岩相互作用模拟研究的实验对象,设计了埋藏成岩过程有关反应的反应物和可能的生成物,并通过热力学相平衡原理,建立了碎屑岩在埋藏成岩过程中各种骨架颗粒(钾长石、钠长石、钙长石以及铁镁暗色矿物中的辉石、角闪石等)在酸性介质条件下发生溶解的固体—流体相化学反应热力学计算模型,并获得了相应的热力学数据库,在此基础上对储层砂岩中主要造岩矿物(钾长石、钠长石、钙长石以及铁镁暗色矿物中的辉石、角闪石等)在埋藏成岩过程的酸性介质条件下发生溶解的热力学习性进行了论述。

It shows the classes and the definiens ofthe intangible assets .The appraisal methods play the role and itslocalization in appraisal works. It chiefly introduces the applicationof the present return value in evaluating the series of prescriptionsin a hospital.

在这一部分,介绍了无形资产的分类、概念,基本的评估方法及各种方法在评估无形资产中的作用和局限性;重点介绍了收益现值法在评估我省某一医院的一系列中药方中的应用,由于此系列药方应用情况不同,在评估中采用的方法也不尽相同,给评估造成一定的难度。

This article take the county territory economy development is the comprehensive construction well-off society s key in as the starting point, the encompassment "the strength county territory economy" the spirit, according to "five overall plans" the principle, through is connected economical theory and so on development theory to the economic in, comparative ad...

本文以县域经济的发展是全面建设小康社会的关键所在为出发点,围绕"壮大县域经济"精神,按照"五个统筹"原则,通过对经济学中相关经济发展理论、比较利益理论、区域发展理论等理论的研究和认识,结合延边朝鲜族自治州的实际,分析了延边州县域经济发展中存在的问题和阻碍县域经济发展的原因,以"农业是稳定的基础,工业是发展的出路"为主线,以政府在市场经济和经济发展中的定位为主要内容,强调了制度在经济发展中的关键和保障作用,衡量一个制度是否有效率的标准应是更多的机会公平和更多的结果效益;强调了在欠发达县域经济中,经济要取得大的和长足的发展,稀缺资源应得到充分的利用、并应向有较大前向效应的工业发展倾斜的观点,并在此基础上指出了欠发达县域经济的发展方向是传统中小工业,同时,针对以往的一些促进经济发展的政策提出了一定的改进的意见;强调了在市场经济条件下,必须更加注重社会保障机制。。。

We meet in this game This is still infatuated with a network Let me be your friends one by one to leave the game To the pursuit of real-life time Then there is a fishes persist in the conviction Let me come to the so-called final Finally, we have a full class to We also become a game of a husband and wife Together to chat together to fight together to see the scenery Appear together in front of friends I have told you several times whether I would like to leave the game But do not know what I come back time and again the Now, I once again decided to leave the game I do not know how long this can leave I do not dare In fact, said to leave the game say that the discussion was not as good as you After the full level because I slowly began to weary of the game Do you have is a day I insist on the belief line Recently, I go to bed every night before you would like to But not like before, like the game's equipment But in reality you imagine how a person What kind of character, and so there We will occasionally illusion of reality can come together The game took a quarter of my college life time Reform is the time to leave As in real life too cruel I felt pressure brought about by the reality Whether in life or learning I really like this now become a regular university, said the University graduate unemployment is equivalent to That is not what I want I am not worried that I can not find a job after graduation Not because of the need to Go on like this I do not even know that after I graduated I can do what I do Looking for a job on it is an impossible dream of Do you still remember I remember a long time ago I asked you I ask you to do what - the sun - the stars - the moon I have become a game where you have the sun I hope I can become your reality stars I would also like to leave after you I hope you will be able to chat with me in the past I hope we can do Haojuhaosan The way the election is himself Life must continue

我们在这场比赛中相遇这是与网路仍痴心让我成为你的朋友们一个接一个,去游戏现实生活时间的追求然后有一个鱼类坚持信念让我谈谈所谓的决赛。最后,我们必须以一个完整的类我们也变成了一个游戏的丈夫与妻子一起,一起聊天,争取一起看风景前面的朋友在一起显示我已经告诉你几次是否我想离开游戏但不知道什麼我回来一次又一次,现在,我又一次决定离开游戏我也不知道多久这可以离开我不敢其实,说要离开讨论不是与你的一样好的游戏说后全部级别因为我慢慢开始厌倦游戏你是我坚持的信念行的一天最近,我去睡觉每天晚上之前您想要但不是象以前,像游戏装置但在现实中你就可以想像如何一人什麼样的字元,并因此有我们偶尔会现实的幻想可以走在一起这场比赛拿了我的大学生活时间的四分之一改革是离开的时候在太残酷的现实生活中的作为我觉得压力带来的现实无论是在生活或学习我真的这样现在成为一个说的正规大学,大学毕业生失业等於这是不是我想要的我不忧虑我不能毕业后找到一份工作不因为的需要这样下去我甚至不知道,我毕业后我可以做我做些什麼找一份工作就是一个不可能的梦想的你还记得吗我记得很久以前我就问你我问你做了什麼--星星-太阳月亮我已经变成一场游戏,在有阳光的地方我希望我可以成为你的现实星星我亦想在此之后你离开我希望您将能够在过去与我聊天我希望我们可以做 Haojuhaosan 选举方式是自己必须继续生活

In any situation whatsoever and wheresoever occurring and whether existing or anticipated before commencement of or during the voyage, which in the judgment of the Carrier or the Master is likely to give rise to risk of capture, seizure, detention, damage, delay or disadvantage to or loss of the ship or any part of her cargo, or to make it unsafe, imprudent, or unlawful for any reason to commence or proceed on or continue the voyage or to enter or discharge the goods at the port of discharge, or to give rise to delay or difficulty in arriving, discharging at or leaving the port of discharge or the usual or agreed place of discharge in such port, the Carrier may before loading or before the commencement of the voyage, require the shipper or other person entitled thereto to take delivery of the goods at port of shipment and upon failure to do so, may warehouse the goods at the risk and expense of the goods; or the Carrier or the Master, whether or not proceeding toward or entering or attempting to enter the port of discharge or reaching or attempting to reach the usual place of discharge therein or attempting to discharge the goods there, may discharge the goods into depot, lazaretto, craft, or other place.

不论任何地方任何情况,不论是在开航前或航程中存在或预料到的,只要承运人或船长认为可能有导致捕获、扣押、没收、损害、延误或对船舶或其货物不利或产生灭失,或致使起航或续航或进港或在卸货港卸货不安全、不适当、或非法,或致使延误或难于抵达、卸载或离开卸货港或该港通常或约定的卸货地,承运人可在装货或开航前要求发货人或与货物权利有关的其他人在装货港口提回货物,如要求不果,可仓储货物,风险和费用算在货主头上;承运人或船长,不论是续航至或进入或企图进入卸货港,或抵达或企图抵达港口通常的卸货地,或企图在此卸货,也可将货物卸在仓库、检疫站、驳船,或其他地方。分析:用来表示遵守的词和词组很多,我们大致列举出像follow, observe, abide by, comply with等。在前面这些词和词语中,comply with是最正式的一个表达,因此,也常常在法律英语中使用。从语法上来说,comply with 的主语通常是物,而其他几个单词或词组的主语通常为人。如

METHODS: Measurement of palatal raphe area first three-dimensional reconstruction of images at up to nasion plane centerline, whichever was determined as a reference surface plane, selection one point on incisor cervix in this line, to measurement of the available palatal bone height; sagittal views were selected at 6 mm dorsally from the incisive canal. The available bone height was defined as the distance from the palatal baseline (lower cortical border of the palate) to the nasal cavity (upper cortical border of the nasal cavity).

先在三维重建图像上以鼻根为准确定面中线平面为参考平面,在此线上选择1个位于中切牙牙颈部的点,在其矢状位图像上,选取位于口腔内侧骨皮质上距切牙孔后缘6 mm处为标测点,通过此点向硬腭做垂线确定腭骨厚度,测量范围自口腔内腭部骨皮质外侧边缘至鼻腔侧骨皮质外侧边缘。

In the frame, Design Structure Matrix is the tool to build up the models of product structure and development process. The product structure topology tree is defined, and the product structure DSM model is introduced. The formalized description about development process model is presented. The characteristics of the tools to build up development process are analyzed, the strongpoint of DSM is discussed, and the development process model based on DSM is introduced. The relation between product structure and development process is analyzed.

在研究框架中引入了产品结构和开发过程的建模工具——设计结构矩阵(Design Structure Matrix, DSM);给出了产品结构拓朴树的定义及其形式化描述,并在此基础上引入了产品结构DSM模型;给出了产品开发过程模型的形式化描述,比较了几种常见的产品开发过程建模工具,分析了用DSM建模工具进行产品开发过程建模的优势,引入了产品开发过程DSM模型;分析了产品结构与开发过程之间的联系,提出了两者之间的集成机制,并在此基础上给出了IM-PSDP模型。

Smith, President of the Church of Jesus Christ of Latter-day Saints, hereby affirm and declare that no such marriages have been solemnized with the sanction, consent or knowledge the Church of Jesus Christ of Later-day Saints; and I hereby announce that all such marriages are prohibited, and if any officer or member of the Church shall assume to solemnize or enter into any such marriage, he will be deemed in transgression against the Church he will be liable to be dealt with according to the rules and regulations thereof, and excommunicated therefrom.

由於有众多的报告中流通,即,多元的婚姻已经进入相反,正式宣布总统伍德拉夫,对1890年9月24号,通常称为'宣言',这是总统发出鲍伯伍德拉夫并通过了教会在大会1890年10月六号,其中禁止任何婚姻违反了法律的土地;我,约瑟夫史密斯总统耶稣基督后期圣徒,在此申明并宣布,没有这样的婚姻被solemnized的制裁,同意或知识的耶稣基督后期圣徒;和我在此宣布,所有这些被禁止的婚姻,如果任何人员或成员的教会应承担向仪式或进入任何这类婚姻,他将被视为对在海侵的教会,他将被判处处理根据条例和细则,及由此逐出教会。

The swelling ratio of the semi-IPN polymer as function of temperature and pH value. A transition temperature of approximately 33.6℃ is obtained. Under the pH value of solution is up to 7 or more, the polymer is more hydrophile.

实验结果得知,在温度敏感性质方面,此semi-IPN水胶在水溶液中随著温度上升,於到达临界温度时会产生一不连续的体积变化,以DSC量测得此温度约在33.6℃左右。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。