在形成的

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

For this cloud seeding case, the glaciation became apparent at cloud tops about 22 minutes after seeding.

而新生水云在形成较早的云迹中没有出现，这些较早形成的云迹在80多分钟的整个观测期间持续扩散。

The results are listed as follows:(1) In peneplain with plenty water-resource, hexagonal spatial structure of urban system can be formed. Due to differential competed function of central places, the hexagonal system is distorted. The result is that the area covered is decr eased and the girth is unchanged——but every border is usually unequal;(2) A pattern of pentagonal system evolves from hexagonal system controlled by a river;(3) A pattern of quadrilateral develops from hexagonal system under two parallel rivers;(4) The equal distance distribution of central places in narrow geographic space has its special reasons. The results are very important for understanding urban's formation and development and urban plan in Huang-Huai-Hai plain.

本文通过自然地理因素中水文、地形这两个重要要素对城镇体系空间结构形成的影响，分析了华北平原区在不同要素影响下的城镇体系空间结构样式：①在水源充足的准"均质"河北冲积平原上，基本满足六边形形成条件，但由于周边中心地发展竞争力不同，故使得正六边形发生畸变——覆盖面积减小而周长却保持不变；②构建了在一条河流影响下的六边形网络结构朝五边形网络演变的模型；③构建了在平行状河流作用下六边形网络向四边形网络演替的模式；④指出了在水源充足的狭长地理空间，城镇体系空间结构发育成等间距的现象与原因。

Through analysis of potential vorticity in isobaric surface field, in high-troposphere a positive potential vorticity disturbance before rainstorm generation;positive potential vorticity disturbance in high-troposphere downward, form one vertical disturbance column, now corresponding precipitation develop stage;When near ground arises negative potential vorticity disturbance, precipitation also weaken.

通过对等压面的位涡分析，发现在暴雨发生发展时，在对流层高层有正位涡向下扰动，在暴雨的发展鼎盛阶段，从对流层中高层到地面形成一个垂直扰动柱；位涡柱对地面气旋的发展有较好的指示性，位涡柱形成时刻前后降水达到最强；当近地面出现负的位涡扰动时，降水随之减弱。

The deep water deposition means the deposition in the area where the water depth is larger than 30 meters, in which the major sediments are coarse sand and gravel. This kind of deposition is the relic deposition formed by the Pleistocene alluvial-lacustine deposition reworked under modern channel currents actions, and ususally the Holocene acummulation lack in the deep waters. The transitional zone deposition is the deposition between the low tidal level line and the 30 meters isobath, where the sediment types is changeable and the grain sizes are gradually coarsening from the low tidal line to the deep water area. This kind of deposition is developed by the deep water eroded sediments sorting under the transitional zone current action. The different types distributed along the isobath. The tidal flat deposition is the fine sediment deposition on the tidal flat which is flo sedimentation of the silty clay under the tidal flat dynamic action and ususally developed fluid mud on the lower tidal flat. The channel mouth deposition is the deposition in the channel mouth area. According to the sediment types, it includes sand deposition where there are coarse sediments supply and silt deposition where there are only fine sediments surpport.

峡道深水区沉积是指水深大于30米的峡道底部沉积，它以粗颗粒砂砾石沉积为特色，是现代峡道水动力对第四纪海退时发育的冲洪积和坡积物进行改造形成的蚀余沉积，全新世沉积地层往往缺失；峡道过渡带沉积是指水深介于低潮位线至30米水深线之间发育的峡道边坡沉积，该带沉积以沉积物类型多样为特色，自低潮位线至30米水深线，沉积物粒级逐渐变粗，由粉砂质粘土逐渐递变为中粗砂，是峡道深水区沉积物经多次分选沉积而发育的，基本平行于等深线分布的条带状沉积，在岸坡较陡岸段，过渡带下部可发育重力沉积；峡道潮滩沉积是指在低潮线与海岸线之间发育的细颗粒沉积，它是粘性细颗粒泥沙在潮滩动力作用下，发生动水絮凝沉积而形成的粉砂质粘土沉积，在低潮滩可发育浮泥；峡道口门区沉积则是在峡道汇入大海或海湾时，在峡道出口水流影响所及范围的沉积，根据峡道口门区供给泥沙的特性，峡道口门区沉积有粉砂质淤泥沉积和砂质沉积两种类型。

Cholesterol gallstones formed in C57L mice and fatty livers developed in AKR mice. Conclusions Biliary cholesterol hypersecretion is the key pathophysiological defect of gallstone formation, lith genes have effects on biliary cholesterol hypersecretion and susceptibility to cholesterol gallstone formation in C57L mice. Lithogenic bile is formed at the canalicular membrane and precedes the development of cholesterol gallstones. It is most likely that cholesterol and bile acid hyposecretion make the AKR strain susceptible to the development of fatty livers and resistant to gallstone formation.

结论胆道胆固醇的高分泌是胆囊胆固醇结石形成的主要病理生理基础，结石基因决定了C57L鼠肝内胆汁中胆固醇的高分泌和胆囊胆固醇结石的易患性，成石胆汁形成在肝内胆管，先于胆囊结石的形成；肝内胆汁中胆固醇和胆酸的低分泌可能与AKR鼠脂肪肝的发生和胆囊结石的免患性有关。

The operating range suitable for three-phase reaction was obtained and a mathematical model established. The results show that MSB formed by fine magnetizable powder and fliudizing flow can be operated stably in a wide range. MSB has three kinds of regimes: particulate, chain, and magnetically condensed. In these regimes, the chain regime is the best for three-phase reaction. When Ni-RE-P catalyst particles mixed with some iron powder, magnetically stabilization is easily achieved; therefore, it is possible that NiRE-P catalyst can be controlled in the bed for reaction more easilly if adding certain percentage of iron powder.

研究结果表明：细粉铁磁性颗粒形成的磁稳定床可在较宽流速范围内稳定操作，该磁稳定床有三种操作状态：散粒状态，链式状态和磁聚状态，其中有利于进行气液固三相反应的操作状态是链式状态；当磁稳定床在最小流化速度和带出速度之间操作时，床层处于链式状态；最小流化速度和带出速度可用（1-24）、（1-25）、（1-27）、（1-33）式计算；弱软磁性Ni-RE-P非晶催化剂中混入定量铁粉颗粒后便可形成磁稳定床，因此利用混入铁粉颗粒在外加磁场作用下控制弱磁性非晶催化剂颗粒进行反应的方法是可能的。

Based on the importance of culture in the operation of the institution,the papers will analyze history and nonce on the cultural atmosphere of our political institution's operation,to find the problems and solutions.

而文化作为在经济、政治、民主习惯、地域环境、宗教信仰与历史长期积淀基础上形成的一种观念体系，是社会系统在观念上和心理上形成的对行为的特定价值取向模式。

The establishment and relaxation processes of the transient gratings, which are formed by two recording 514nm beams with different polarization directions, SS, PP, SP and ±45℃, are investigated. Using the weak absorption of the chromophores at 632. 8nm and the positive dichroism of the chromophores molecules, a kind of bi-photonic transient gratings, owing to the periodic distribution of Trans and Cis isomers, is established first time in azo-dye doped polymeric bulk sample.

实验考察了两束514nm的写入光在不同偏振情况下（SS，PP，SP和±45），在样品中形成的瞬态栅的建立和弛豫情况；利用偶氮生色团在632.8nm波段的微弱吸收和生色团分子的正二向色性，首次在块状偶氮聚合物材料中形成一种基于生色团反式和顺式异构体密度分布的双色光瞬态栅；利用瞬态栅的快速建立和弛豫，考察了其在衍射型全光开关方面的潜在应用。

The material for formation of the granitoids isderived from mixing sources of crust and mantle, with decreasing in crust-derived componentsfrom early to late.5. The tectonic setting for formation of the Mesozoic granitoids in the region isdefined as a post-collisional setting. The occurrence of a small part of rocks with characteristics ofcollisional or anorogenic granitoids provides evidence for the continuous process oftransformation of tectonic regime from collisional through post-collisional to anorogenic.6. Themultiple cycles of mantle-derived magma underplating caused by the multiple stages oflithospheric thinning in the Mesozoic result in the partial melting of middle to lower crust orcrust-mantle syntexis to form magmas. The crystallization of these magmas emplacing afterfractionation is responsible for the formation of the Mesozoic granitoids in this region.

形成花岗岩类的成岩物质来自壳幔混源。5、河南省西部地区中生代花岗岩类成岩构造环境为碰撞后环境，部分岩石具有碰撞期或非造山期花岗岩的某些特征，表明碰撞后阶段既是碰撞造山过程的延续，又是向非造山环境的构造转换时期，因而其岩浆活动在一定程度上仍然继承了碰撞前消减时期和碰撞时期已经活化的岩浆源区性质。6、研究表明，区内中生代多阶段的岩石圈减薄作用引发多旋回的幔源岩浆底侵以及中下地壳的部分熔融或壳幔同熔，由此形成的岩浆经分异演化后侵位可以形成区内的中生代花岗岩类。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。