在应用

Electrocatalysis can be considered as one of the most popular application of selemium nanoparticles and carbon nanotubes. In this paper, the potential applications of MWCNTs/Se modified electrode in biological electrocatalytic were investigated and discussed.

根据碳纳米管和纳米硒在生物电催化反应中表现出的巨大潜能，研制了碳纳米管复合纳米硒修饰电极并探讨其在生物分子电化学研究中的应用，对发展新型电化学传感器和拓展纳米材料的应用具有重要的理论意义和实际应用价值。

The applications of DGGE were mainly introduced among the food microbiology field,in assessing microbial communities in fermented product s and variations during food fermentation,differentiating ferme.

本文概述 DGGE 的基本原理、发展和应用现状；着重阐述该技术在评价发酵食品的细菌群落组成和动态变化、区分发酵食品、控制食品质量、检测食源性致病微生物等食品微生物研究领域中的应用；进一步展望了 DGGE 技术在食品研究领域的应用前景。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

However, current WSN applications in Precision Agriculture all adopt the traditional client/sever model, data are sent from the source to the destination, because of hugeous data, high concurrency and strong signal interference in Precision Agriculture and the extremely limited sensor network resources, such client/sever model hampers WSN applications in Precision Agriculture.

然而当前无线传感器网络在精细农业中的应用都采用传统的客户/服务器模型，数据从源端发送到目的端，受精细农业中数据量大、并发性强、信号干扰强及传感器网络资源极端受限的影响，这种客户/服务器模型阻碍着传感器网络在精细农业中应用，导致了当前传感器网络大规模成功应用于精细农业的例子很少。

Our experiment aim at use the acupuncture method"Wang's Jiaji points"which created by the famous doctor Leting wang from Beijing hospital of TCM at the early days of the stroke patients to prevent and treat hypermyotonia,prevent muscle spasm or lessen the spasm level,to improve the stroke patient's ADL.that study is a prospective , randomized, double-blind,parallel-group,use the random digits table method,and compared with the"twelve points of hands and feet",which used frequently at acupuncture department of Beijing hospital of TCM.we use the NIHSS scale, improved Ashworth scale,domestic Fugl-Meyer scale, castigatory MBI scale to score the patients'muscular tension and motor function improvement condition.

本试验旨在在中风病人患病早期应用北京中医院名老中医王乐亭先生的&王氏夹脊穴&预防性治疗肌张力增高，防止肌痉挛的出现或减轻其痉挛程度，以改善中风患者的生活质量。在试验设计方面以北京中医院针灸科目前治疗中风应用较多的&手足十二针&为对照治疗方法采用随机、单盲、平行对照设计。并应用NIHSS、改良的Ashworth量表、国内简化的Fugl-Meyer运动评分法、修订的巴氏指数评分方法对患者治疗前后进行评分以了解患者肌张力增高情况及运动功能改善情况。

Choose medicaments correctly, reasonable use medicaments, in order to make sure with medicine safety is mixed effective will be henceforth the main task that clinical medicine learns, for solution control hospital the clinical current situation that use drug reachs existence issue, promote the development of the reasonable job that use drug, raise a hospital reasonable the level that uses drug, the prescription of two place has outpatient service of author general hospital, be in hospital analytic research, summary goes out unreasonable a few big states that use drug, undertake classified at the same time. 1 clinical unreasonable with medical analysis each division all applies 1 abuse of 1 · or antibiotic of blind application antibiotic extensively, utilization rate amounts to 70% above, among them couplet is used rate be as high as 40%, appear even 2 couplet, triplex above, the commonnest abuse is infection of the upper respiratory tract, be admitted to hospital give 2~3 to plant antibiotic cure. 1 · of 1 · 1 without point to this kind to the cure that ask for uses drug unreasonable with medicine main show is in the person that the virus sex appeal such as hepatitis of sex of common cold, chicken pox, berpes zoster, virus is caught, in infection of not amalgamative bacterium, point to without what apply antibiotic ask for next using medicine. Incontrollable virus affects antibiotic, increase hepatic burden instead to patient of virus sex hepatitis.

梗概： 正确选择药物，并合理使用药物，以保证用药平安和有效是今后临床药学的重要任务，为了解把握医院临床用药目前状况及存在新问题，推动合理用药工作的发展，提高医院合理用药的水平，笔者将医院门诊、住院两处的处方进行分析探究，总结出不合理用药的几大状况，同时进行分类。1临床不合理用药的分析1·1滥用或盲目应用抗生素抗生素各科均广泛应用，使用率达70%以上，其中联用率高达40%，甚至出现二联、三联以上，最常见滥用为上呼吸道感染，入院给予2~3种抗生素治疗。1·1·1无指征的治疗用药这类不合理用药主要表现在普通感冒、水痘、带状疱疹、病毒性肝炎等病毒性感染者，在未合并细菌感染，无应用抗生素的指征下用药。

The article discussed upon the interactive design which can be used as an innovatory mean in the show of popular science.

文章围绕科普展示这一专业领域，论述了交互设计在该领域应用的相关概念、应用价值、实践意义和程序方法，同时列举了国内外目前交互设计在展示设计中应用的实际案例，描述了科普展示中交互设计的发展现状和趋势。

Combining with practical application, they also gave an introduction of modernized computer latticing technology which applied to modern project management measures for discussion.

根据甘肃火电工程公司在张掖电厂2 300MW 机组工程建设中现代化信息管理技术的实际应用，论述了在大型火电站安装工程中推行工程信息化、网络化管理的情况，并结合实际应用阐述了运用现代化计算机网络信息技术进行工程项目管理的应用，并对有关问题做出探讨。

Besides the introduction of concept about pyroligneous acid,the field of application,the key point is to expounding utilization status of pyroligneous acid in vegetable,crops,edible mushroom,livestock and poultry.,aimed at extending and study of pyroligneous acid in agricultural production.

本文除介绍木醋液的概念、应用领域及发展历史外，重点阐述了木醋液在蔬菜、大田作物、食用菌和畜禽上的应用现状，旨在推动木醋液在农业生产上的推广应用和研究。

The major achievement of this paper is: Based on characteristics of the traffic data distribution, execute pattern recognition operations on traffic condition on two dimensions by clustering, then use BP neural network to describe and forecast traffic flow aiming at each pattern. Making use of classic flow-occupancy inverse "V" model, implement polynomial fitting using least-squares algorithm and statistics method on flow curves to detect outliers which are proved to be not accord with practice through the actual implement, then use the moving average model to recorrect the outliers and absent. Make correlation analysis on muti-direction flow queues of the intersection and ones of upriver intersections, choose flow queue with high correlation as assistant one to improve the error tolerance of the prediction system, at the same time we can use the method to give an estimation of flow in intersection with out sensors. We design and implement an SOA(Service-Oriented Architecture)-based UTDD(urban traffic data mining development) with high expansibility and performance, which implement unified management and call of the data-mining application though defining a XML-based description of data-mining process and a common interface to call data-mining process, finally we build traffic flow prediction application model on UTDD.

根据交通流量数据分布的特征，提出基于k-means的二次聚类方法，对交通流量在流量大小和时间上进行模式划分，进而对各个交通流模式进行基于BP神经网络的描述和预测，从而提高模型对流量预测的精度； 2）根据流量/时间占有率倒&V&字形曲线分布模型，提出基于最小二乘法的三次多项式曲线拟合和统计方法的异常检测方法，实际应用表明该方法能够有效识别异常数据，然后根据移动平均算法对异常数据进行修正； 3）基于序列相关性分析，分别对预测方向的交通流量数据序列、上游路口相关序列以及预测路口其它各个方向上的交通流量序列进行分析，选择相似性流量序列，作为辅助序列提供其他没有检测器路口的流量估计； 4）设计和实现了基于SOA（Service-Oriented Achitecture）的高性能、可扩展的智能交通数据挖掘系统UTDD，该系统通过定义基于XML的数据挖掘过程描述和通用的过程模型接口，实现数据挖掘应用的统一管理和调用，最后在UTDD上建立了基于路口流量预测的应用模型。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。