在实验上

In a two-step incubation experiment, high and low temperatures were combined with RH 70%, RH 80% and RH 90% to treat two silkworm races, Bibo x Dongtin and Chunlei X Zhenzhu, to investigate the causes for uhe occurrence of reversed silkworm in sericultural production and its hatching pattern.

针对生产上出现&逆出蚕&这一情况，通过催青实验调查发生逆出蚕的原因及其孵化方式。实验采用碧波×洞庭、春蕾×镇珠 2个蚕品种，分别在常温、高温与RH 70 %、RH 80 %、RH90 %组合的 6个条件下，进行两段式催青至孵化。

An experiment on forming of cross-hatch on the ablation material surface has been done under the condition of Mach 5 and tur-bulent boundary layer regime.Artifitial disturbances created by randomly setting several pins along a line perpendiculer to the flow direction have shown stochastical character of local cross-hatch and independence of global orderly structure,therefore the pattern appeared fractal behavior.

在外流M＝5湍流边界层状态下，模型为170×300mm￣2蜂蜡平板上进行了烧蚀花纹图案生成的实验研究，通过实验证明了人工安置干扰源并不影响烧蚀材料表面花纹的有序结构，对大面积烧蚀花纹的分析表明，局部花纹结构具有随机性质，因此烧蚀花纹具有分形结构的特性，用分形方法可以大致模拟花纹生成图案。

Methods The third cranial nerve was dissected and repaired at the subtentorial area (group Ⅰ, n = 24) and the supraorbital fissure (group Ⅱ, n = 24), the functional recovery was evaluated by measuring the horizontal and vertical vestibule-ocular reflexes.

实验Ⅰ组大鼠（n = 24）经幕下、实验Ⅱ组（n = 24）大鼠经眶上裂干预动眼神经，术后通过前庭眼反射评估眼外肌在垂直、水平方向的恢复程度。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

New method that Biological Tissue Section tryptic digestions in situ coupled with imaging mass spectrometry was established.

通过正交设计实验，优化实验条件，我们建立了在福尔马林固定组织切片上加胰蛋白酶液(0.1ug/ul)，60℃超声10分钟进行修复提取，再直接质谱扫描分析的新方法。

Low resolution was obtained in genetic variation investigation of different cowpea cultivated germplasm of Vigna unguiculata based on ITS sequence data, while good results could be obtained in genetic diversity and phylogeny of differern species of genus Vigna.

RAPD可以用于种下的遗传多样性研究。但基于随机引物的RAPD-PCR扩增，受实验中各种因素的影响较大，导致数据获得的重复性不是很好、且不可移植。因此只能作为一种辅助方法。从技术上讲，为保证实验条件的一致性，应该一次PCR反应中对所有的研究对象进行扩增，并在扩增片段的电泳分离检测时尽量保持条件的一致。

The expressions of VEGF、bFGF、IL-1βin myocardium were measured by RT-PCR and ELISA. Other steps and methods were same with the second part. Results: 1. The cells in 2Gy, 4Gy groups and unirradiated group grew well. All cells in 8Gy and 12Gy groups were dead after irradiation.

30只大鼠随机分为注射上清液组和培养基组，除了采用RT-PCR和ELISE两种方法检测VEGF、bFGF、IL-1β在心肌组织内的表达外，其他实验步骤、方法及检测指标同实验第二部分。

Compared with the method of Lg[N00-N], the method of Lgderivative is more accurate. A simple and flexible signal source of multiple decay was designed with the linear adding of many RC charging signals. Two suits of multiple decay data which are acquired independently over a 10th order time span indicate that, the shape of the Lg derivative peak is coherent with its theoretical peak unitarily.

独立进行的两组不同的10 个时间数量级的数据都表明，复杂衰减过程的实验数据的K-Lg微分峰形曲线，与以各元件RC 值计算出的衰减寿命值和测量各单一充电过程的衰减量实验数据为参数，再由理论公式产生的曲线比较，两者在曲线整体形状上有很好的一致性。

Based on the experimental data of the displacement of oil by water in the Liuchu Oilfield and other relevant experimental data, the calculation of univariant facies differentiation indexes indicates that the interference from porosity and permeability can not be eliminated.

在留楚油田沙一段储集层的水驱油实验和其他相关实验测定数据的基础上，进行单变量相分异指数的计算，结果表明孔隙度和渗透率对最终驱油效率的影响并不能通过分沉积微相来消除。

The newly discovered endogenous vasodilating and diuretic peptide adrenomedullinwas considered to be of attractive value in clinical treatment of hypertension and congestive heart failure.

为探索通过体内表达肾上腺髓质素(adrenomedullin， AM)治疗高血压和慢性心衰的可能性，本实验构建了重组AM真核表达载体，并在无内源性AM表达的凡62细胞株上进行了体外表达实验。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！