在实验上

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Ground on the actual conditions of CAI, CSE and physical experiment teaching in our country, a new-style simulant physical experiment-teaching mode that based on independent instruments and components is offered in this paper. This mode is devised according to the essential CAI educational theory-the Constructivism also. The most distinct advantage of this mode is, with enough simulant instruments and components, students can not only implement experiments according to the textbooks, but also design and carry out new experiments by themselves. Students experiment interest can be activized and their creative thinking and competence can be improved.

本文在分析了我国计算机辅助教学、计算机仿真实验和物理实验教学现状的基础上，根据计算机辅助教学的基本教育理论-建构主义，提出了一种全新的仿真物理实验开发模式-基于仪器和元器件的开发模式，指出了其最突出的特点，即在提供充足的实验仪器和元器件的基础上，学生不仅可以按照教材上的实验方案，也可以自己设计实验方案进行实验，甚至还可以自己设计并实施新实验，从而充分调动学生的学习兴趣，并由对仿真实验的兴趣过渡到对实物实验的兴趣，以弥补我国学生实验条件差、实践机会少的缺陷，培养他们的动手能力和创新能力。

In the pre-experiment stage, we analyze the colors and shapes of 135 visual illusions by Kitaoka, a Japanese scholar. The result is referenced in the formal experiment. Formal experiment includes three sub-experiments: Experiment 1 is to discuss the effect of the displacement of disjunctive center on visual illusions' movement; Experiment 2 is to discuss the effect of image layout on visual illusions' movement; Experiment 3 is to discuss the effect of color configuration on visual illusions' movement. Post-experiment stage is a repeat of formal experiment to confirm the results.

前测实验是进行日本学者北冈明佳的135幅错视动态作品之造形与色彩的分析，并将分析结果作为正式实验样本之参考；正式实验分成三个实验进行探讨，实验一在探讨分割点中心的位移对於错视图形在视觉动态上的影响，实验二在探讨图形配置对於错视图形在视觉动态上的影响，实验三在探讨色彩配置对於错视图形在视觉动态上的影响；而后测实验则是将正式实验之实验结果进行再实验，以印证正式实验之结果。

Using such NMOR effects, rotation angle variation with the magnetic field can be got, even up to an order of magnitude 10-13 T.

利用这一效应，同时在理论上获得了不同于以往理论及实验的双峰色散曲线。

The catalytic distillation for the reaction of isopropanol dehydrogenation was conducted experimentally in a pilot plant.

在实验装置上对异丙醇脱氢的催化蒸馏过程进行了研究。

The simulation experiment shows that the algorithm can cause the detail of image becoming true and rich, and make polarization characteristic becoming sufficiency after fusion. At the same time, it can reduce pretreatments of original image, and take a great visual effect on the whole.

仿真实验结果表明，这样使得融合后的图像细节更真实更丰富，图像的偏振特性体现更为充分，同时减少对源图像的预处理要求，使图像在整体上有较好的视觉效果。

These conditions may be used as the basis for determining the initial dimensions of the main components of transducers.

文章还在实验的基础上，提出了一种推求变截面构件刚度平均值的力学方法，称之为&换算刚度法&。

And in science, as in common life, our confidence in a law is in exact proportion to the absence of variation in the result of our experimental verifications.

正如日常生活中一样，在科学上，我们对于规律的信心是与实验结果的一致性完全相符的。

This experiment technique provides a new method to study high J Rydberg states and autoionization state of atom.

在实验技术上，提供了一种研究惰性气体原子以及其它原子高里德堡态和自电离态的新方法。

For the chemical shifts of the carbons that lay in the conjugated sketeton we obtained a good linear relationship between the calculated and experimental data as well as their antimagnetic contribution σ_d and electronic density ρ.

对于固定在π骨架上的碳原子，其化学位移计算值与实验结果有很好的线性关系，并且它们的反磁性贡献σ_d和电荷密度ρ之间也有较好的线性关系。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。