在后的

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The load-displacement plot was measured and analyzed at elbow flexion of 90, 60, and 45° and under four conditions (intact elbow, type-I coronoid process fracture, type-I coronoid process fracture with AMCL deficient, and type-II coronoid process fractures with AMCL deficient).

N单轴压缩实验，分别在屈肘 90、60 和 45°测量标本在下列情况下的负荷-位移曲线：①完整肘关节；②Ⅰ型冠突骨折后肘关节；③Ⅰ型冠突骨折合并 AMCL 损伤后肘关节；④Ⅱ型冠突骨折合并 AMCL 损伤后的肘关节。？

The Go moved backward with 1.35mm and upward with 1.90mm in group I, the gonial angle increased for 3.69°, and angle between palatal plane and mandibular plane was increased for 5.00°; In group II, the Go moved backward with 1.85mm and upward with 3.32mm , the gonial angle increased for 4.06°, and angle between palatal plane and mandibular plane was increased for 5.22°. Both in group I and II, there was a tread for clockwise rotation of mandibular corpus. The length of pterygomasseteric sling was mild increased due to the backward movement of Go.

在Go点的变动方面，在Me点向前位移的族群里，术后Go点平均向后1.35mm，向上1.90mm， gonial angle 平均增大3.69度，腭平面与下颚平面的夹角则是增加5.00度；在Me点向后位移的族群里，术后Go点平均向后1.85mm，向上3.32mm， gonial angle 平均增大4.06度，腭平面与下颚平面的夹角则是增加5.22度，不论是向前或是向后的族群，结果皆显示翼咀嚼肌悬吊带有轻微被拉长的迹象。

The changing trend of progesterone during oestrous cycle resembles with that during the first 14d of early pregnancy. Its content reaches the peak value at lOd after oestrus and then declines. The estradiol peak appears at 3d of oestrous cycle, and the level rises again before oestrus. The concentration of LH declines after oestrus. The level of follicle stimulating hormone reaches the peak value at lOd after oestrus (2.40mIU/ml).

发情周期：民猪发情周期孕酮的含量与妊娠早期14天内的变化极其相似，在发情后第10天达峰值，而后开始下降；雌二醇水平在发情后第3天出现高峰，下次发情前开始升高；促黄体素在发情当天达最高水平，然后开始下降；促卵泡素在发情后的第10天达到峰值(2.40mIU/ml)。

The materials co-cultured with agrobacteriumand cultured transitionally were transferred to screening mediumand 15 adventitious buds were obtained after60d and all of themrooted after they were cultured in rooting mediumfor 20d.

将经农杆菌共培养及过渡培养后的材料转入筛选培养基中，60d后获得15个不定芽，再在生根培养基中培养20d后，全部生根。

Methods 38 cases with 53 hips of ANFH treated with the method of thrombolysis and opening catheterizing and perfusing medial and lateral circumflex arteries.Those drugs are Urokinase 200 thousand Units,Danshen Root 20 ml and Ligustrazine 20 mg. Retention catheter was held in the target vessels and infused the same drugs besides Heparin 4500u in 3 days. To evaluate therapeutic effect ,the DSA of the femoral artery and x-ray plain film and/or CT/MRI of the femoral head were performanced. Results All cases were followed up 1~16 (mean 9.5) months.

方法对38例ANFH患者的53髋先常规行股动脉DSA，后在股骨头的供血动脉旋股内、外侧动脉内灌注溶栓、扩血管药物，即在每支血管内注入稀释后的尿激酶 20万u、丹参10 ml及川芎嗪20 mg；术后保留导管于动脉内，经导管每日注入同剂量的药物及肝素钠4500 u，连用3天后拔管，治疗后复行股动脉DSA检查及行股骨头X线平片及/或CT、MR随访以评估疗效。

Roasted goose string is made by goose meat, goose skin and some bowels, which is stringed on a stick after cutting, then seasoned after steaming, finally roasted.

烧鹅串是以鹅肉、皮和某些脏器为原料，切块后穿在竹签上，蒸熟后调味，经烤炉烧烤后的系列方便肉制品。

In control, however,the weak expression ofCY3 mRNA can be found after 48 hours of chilling and showed little increase at the third day instress.

非逆境条件下，IPT对秧苗根系中mRNA的表达差异与对照相比没有很大的变化；低温胁迫3天后，IPT对根系中mRNA的表达影响很大，既能增强某些基因的表达，又能抑制另一些基因的表达；用DDRT-PCR技术，寻找到5个低温胁迫下在对照和IPT处理中差异表达的基因，其中CY3片段与水稻CDPK1和CDPK2的mRNA有很高的同源性，初步断定CY3在植株的抗逆反应中可能具有与CDPKs相似的功能；在Genbank中没有检索到与其它4个片段同源性比较高的已知序列；IPT处理中CY3表达量在低温处理24hr就有明显升高，以后随胁迫时间的延长其表达量不断加强；恢复常温后，CY3表达继续增加，在恢复第14天开始下降，下降速度缓慢，IPT对CY3的影响一直延续到苗末期，对照中CY3在低温胁迫48hr才有微弱表达，72hr后表达量并未增加许多；恢复常温后第4天就开始下降，很快降到最低。

RESULTS:①A total of 178 injected and survived zygotes were replanted. Thirteen mice were born with the birth rate of 7%.

结果：①178只注射pcDNA3.1-mutant LMNA后的存活受精卵移植至假孕鼠输卵管后，出生13只子代鼠，出生率只有7%；58只注射pcDNA31 -mutant LMNA后的存活受精卵移植到假孕鼠输卵管后，在移植后12~15 d的受体母鼠子宫内收集到1只活胚和5只死胚。

Under the microenvironments of fruit bagging,Alternaria had always been the major fungi in different bags,sites of fruit and weather conditions during the whole growing season. As the secondary major fungi,Penicillium had always been after July and emerged later in inner bag and cortical pore than other sites.In the bag with which the inner bag was red and waxing,Trichoderma was isolated first in pericarp without cortical pore and inner bag as well as later in cortical pore after August.In \'Kobayashi\' bag which inner bag was red and waxing, Aspergillus and Fusarium were isolated in pericarp without cortical pore and cortical pore after bag removed.Alternaria and Penicillium were isolated in unbagging fruit and \'Qianwei\' bag which inner bag was red and unwaxing and the date Penicillium was isolated in CK was 45d later than bagging treatment.Under high temperature weather condition,there were no differences in the fungi population structure on fruit surface among all treatments.And the fungi population structure on fruit surface in bagging apple was more than that of unbagging ones under overcast and rainy condition.

在苹果套袋微域环境下，不同育果袋、果实不同部位、不同时期、不同气象条件下的真菌主要是链格孢霉，且在套袋后的整个生长季均可分离到；青霉为第二大真菌，在7月份后均可分离到，在非皮孔部位的出现要早于内果袋和皮孔部位；8月份后在内袋红色涂蜡的育果袋上分离出了木霉，其先出现在非皮孔处和内果袋，然后出现在皮孔处；曲霉和镰刀菌仅在内袋红色涂蜡的&小林&袋的非皮孔部位和皮孔部位于摘袋后分离出；在内袋为黑色且未涂蜡的育果袋和未套袋果上仅仅分离出链格孢霉和青霉，且青霉分离出的时期要比套袋果晚45d。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失