在后

A three-mask chip process was first attempted in this work, where the 3rd mask was designed to evaporate the identical metal films for p- and n-pads. However, it was found that the Cr film showed inferior adhesion property on the ITO current spreading layer after the peeling test. In order to overcome this problem, a contact aperture was opened in the mask of ITO layer.

改为三道光罩后，P、N电极使用同一道光罩，并蒸镀相同的金属，但实验后发现，铬和氧铟锡的附著性并不好，经黏贴测试后，发现有电极剥落的情形，所以我们在透明导电层的光罩上，做了个圆孔的设计，这个圆孔的设计可以得到二个优点。

In the early morning, in a house-jacket, she looked after the management of her household. Then she drove on saints' days to Mass, and from Mass to the gaols and prisons; and of what she did there, she never spoke to any one. On ordinary days she dressed and received petitioners of various classes, of whom some sought her aid every day. Then she had dinner, an abundant and appetising meal, at which some three or four guests were always present. After dinner she played a game of boston; and at night had the newspapers and new books read aloud to her while she knitted.

大清早，她就穿上短棉袄，搞一点家务，之后，每逢节日去做日祷，日祷完毕后便去寨堡和监狱，她在那里从事什么活动，她不向任何人透露，在平日里，她穿好衣裳后，便来招待每天到她家里来的各个不同阶层的向他求援的人，然后用午餐，在味美而丰盛的午餐上，经常有三四位来客，在午餐之后打一圈波士顿牌，晚上叫人给她读报，给她读新书，她一边听，一边做针织活计。

And E.N.E: Had they seen the Island, as I must necessarily suppose they did not, they must, as I thought, have endeavour'd to have sav'd themselves on Shore by the Help of their Boat; but their firing of Guns for Help, especially when they saw, as I imagin'd, my Fire, fill'd me with many Thoughts: First, I imagin'd that upon seeing my Light, they might have put themselves into their Boat, and have endeavour'd to make the Shore; but that the Sea going very high, they might have been cast away; other Times I imagin'd, that they might have lost their Boat before, as might be the Case many Ways; as particularly by the Breaking of the Sea upon their Ship, which many Times obliges Men to stave, or take in Pieces their Boat; and sometimes to throw it over-board with their own Hands: Other Times I imagin'd, they had some other Ship, or Ships in Company, who upon the Signals of Distress they had made, had taken them up, and carry'd them off: Other whiles I fancy'd, they were all gone off to Sea in their Boat, and being hurry'd away by the Current that I had been formerly in, were carry'd out into the great Ocean, where there was nothing but Misery and Perishing; and that perhaps they might by this Time think of starving, and of being in a Condition to eat one another.

如果他们发现这个小岛，我想他们一定会用船上的救生艇竭尽全力划到岸上来的。但看来他们一定没有看到小岛，只是鸣枪求救，尤其是他们看到我燃起的火光后，更是多次放枪。由此我头脑里出现了种种设想。首先，我想到，他们看到我点燃的火光后，必然会下到救生艇里拼命向岸上划来，但由于风急浪高，把他们刮走了。一会儿我又猜想，也许他们的救生艇早就没了，这种情况是经常发生的。当大船遇到惊涛骇浪时，水手们往往不得不把船上的救生艇拆散，甚至干脆扔到海里去。过会儿我又想，也许与他们结伴同行的船只，在见到他们出事的信号后，已把他们救起来带走了。我又想到，说不定他们已经坐上救生艇，可是遇到了我上次自己碰上的那股急流，给冲到大洋里去了。到了大洋里，他们可就糟了，那是必死无疑的。说不定这会儿他们都快饿死了，甚至可能正在人吃人呢！

Questionare about asthenopia was filled by the patients immediately after laser treatment and one day, one week and one month after using the eyedrop. Side effects of the eyedrop were also observed.

对 60例91眼激光治疗后使用七叶洋地黄双苷滴眼液的患者，分别在激光后即刻、用药后1d；1wk；1mo进行视疲劳症状问卷调查，同时观察药物的不良反应。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

On an average, the range of the motion of augmented vertebral boody was reduced by l8％(from 0.475mm to 0.394mm) under physical load.

移位场变化：〓椎体增强后，在生理负荷作用下，增强后移位为0.394mm，未增强前平均移位约为0.475mm，即增强后使椎体移位下降18％（0.475mm—0.394mm）。

After anesthesia, the guinea pigs'scalp surface was exposed and two stainless screws (5 mm length and 1.2 mm diameter) were implanted at 6 mm in front of and 10 mm behind bregma, as reference electrode and recording electrode. In the middle, a third stainless screw was implanted as ground electrode. 3 days later, awaked guinea pigs were fixed in multifunctional head-body apparatus. RETI-port system was used to record PVEP of control group and experiment group.

麻醉后，在前囟前6 mm、后10 mm及矢状缝正中处钻孔，均植入长5 mm、直径1.2 mm的不锈钢螺钉作为参考电极、记录电极和地极。3 d后，将清醒状态下的豚鼠固定于自制多功能头身一体化固定台面上，采用RET I-port系统(Roland Consult，德国)分别记录对照组和实验组图形视觉诱发电位的变化。

The experiment results prove that the boiler coking is largely reduced and the temperature difference of two sides of horizontal gas-pass is very small, when the 330MW boiler operate, the pressure of primary hot air parison is controlled beyond 12.0～12.5KPa, the O2 content of air pheheater is controlled about 3.0%, the door of OFA is opened to 30%, the buffle of the inner secondary air sleeve is adjusted to the minimum, the outer secondary air swirling strength and the baffle of the outer secondary air sleeve is regulated to the maximum and the outlet temperature of pulvervised mill is kept in 70℃.

试验结果表明，330MW运行时，一次热风母管压力控制在12.0～12.5kPa以上、空气预热器实际氧量控制在3.0％左右、燃尽风门开至30％、内二次风量套筒挡板调整在最小、外二次旋流强度和外二次风套筒挡板调整到最大、磨煤机出口温度维持在70℃，此时锅炉结焦大幅度减轻，水平烟道两侧烟温差非常小。优化试验完成后，进行了单烧灵武烟煤对比，结果是：掺烧高灰熔点的银北煤后，炉膛结焦大幅度减轻，锅炉过热器减温水量较小，主蒸汽压力易提高，带负荷能力正常；再热器烟气挡板开度较大、减温水量较小；风机电耗较小。

When scales are proposed by nutrition solution, the information of bulblet is very regular: information of bulblet is almost located in inside 1—2mm of wound, the root is formed below of bulblet.

营养液浸种鳞片，小子球的分化很有规律性，据试验观察，小子球发生大都在伤口腹内侧1—2mm处，在沿切口呈线状排列，腹外侧和愈伤组织处只占1%左右，小子球形成后多在其下部生根；而一般基质无菌水培养的多先在鳞片下部生根，后形成小子球，即较前者推迟一周。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。