在后

I lose and I gain; I make mistakes and I learn from them; I feel puzz led and I take a tumble...

我失去后获得，我犯错后增智，我困惑后顿悟，在库柏的每一天我都在成长。

On the PC of the Windows Operation system, the system of 3D reconstruction has been designed by Visual C++ programming language. It includes 3D data import, display of the original and pre-process CT images, acquirement of the data showed vividly on the screen by the improved algorithms about MC and Ray Casting and mutual observation through magnification or demagnification and circumvolving.

论文以此为基础，在PC 上，基于Windows 操作系统，利用Visual C++开发工具，运用计算机图形学和图像处理技术，实现了三维体数据的输入；二维CT 图像数据的显示和预处理；通过阈值对CT 数据进行了分割；运用改进后的算法分别对分割后的数据进行了面绘制和体绘制；对绘制后的数据在屏幕上形象逼真地进行了显示；对显示的数据进行交互式的观察，如旋转、放大、缩小。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

When the mevinphos was utilized for a long time in highland barley field, the young-root of next-season cole shortened 1.9~2.0cm and fresh weight decreased 0.13~0.14g per individual plant compared with the control. Low-tillage in time after autumn-harvest showed that the weed seeds in the ground surface were harrowed into the tilth of 0~10cm. Early-low-tillage in spring-sow of the next year induced weed seeds of soil surface to germinate and come out ahead. After 10 days, tillage-sow can decrease about 40% weeds. Compared to the normal plough-row, direct late-tillage-row was postponed 10 days, and can decrease about 30% weeds. In the seedling period or later, rudimental weed seeds were handled with a hoe, which led to better cure and control effects and had more benefits to the young seedling growth and yield of the next-season crop.

研究结果表明：氟乐灵在油菜田长期使用时，土壤残留农药抑制下茬青稞幼根生长，幼根较对照株缩短3.0~3.1cm/株，鲜重量减少0.19~0.20g/株；甲磺隆在青稞田长期使用时，下茬油菜幼根较对照株缩短1.9~2.0cm/株，鲜重量减少0.13~0.14g/株；秋收后及时浅耕可将落入地表的杂草种子全耙入0~10cm耕层内；翌年春播早浅耕可诱发土壤表层的杂草种子提前萌发出苗，10d后耕种，能防除40%左右的杂草株数；较正常播种期推迟10d后直接晚耕播种，可防除30%左右杂草株数；苗期或后期拔出残余杂草种子时，除草效果理想，对下茬作物幼苗生长发育及产量无任何不良影响。

After treatment with chlorfluazuron to the larvae, the activities of POD in the larvae of three instars were all higher than the control, while the activities of POD in the second and third instar were only 65.0% and 99.2% of those in the control.

抑太保处理后，过氧化物酶活力除2龄幼虫在处理后5 h下降，仅为对照的65.0%，3龄幼虫在处理后1 h为对照的99.2%外，3个虫龄幼虫的POD活力均高于对照。H2O2由0增加至0.1 mL，POD活力呈线性关系上升。

The results show that some twin crystals and honeycomb structures appear in the shear plane and many kinds of deformation mechanism exist such as extruding, kinking and so on. When annealed at 550 ℃ for 2 h, the recrystal gains are observed in the shear plane. Especially the side receives the cut of guillotine, the size of recrystal grain is very thin, corresponding with the recrystal grains obtained by cold rolling at 80% deformation rate.

结果表明：经剪切变形后，切面处形成一种独特的带状变形孪晶和蜂窝状变形组织，且存在挤压、扭折等多种变形机制的作用；合金在550 ℃退火2 h后，切面出现许多再结晶晶粒，且在承受剪床切口的一边再结晶晶粒非常细小，相当于冷轧变形80%后的再结晶晶粒尺寸。

For example, MI behaved most sensitive to heart state when spontaneous respiration inhibited with respiration machine; FD could manifest heart healthy level, which reduced sharply sooner after acute injury but remained almost steady afterwards; the parameters of Poincare dissert-dot plot and HRV could accurately distinguish the state change before and after ligaturing; the HF-ECG excelled in not only revealing injury degree but also locating injury position.

例如，交互信息对自主呼吸被接呼吸机抑制后的状态敏感，分维具有表征心脏健康状况潜力的特性，在急性损伤后明显减少，但不随损伤的时间延续而变化；心率变异性参数和散点图可以灵敏地区分出急性损伤前和损伤后的状态；而高频心电在表征心脏损伤和定位方面独具特色。

The weight of fish sample and marinade were equal, and the temperature of marinading were retained 3 + l℃,15d later measured the water capacity, crude fat, crude protein, crude ash content ,TMA,PH,TVB-N and TBA, sodium chloride ,sensing of the fish sample from different marinade. The results showed that the basic chemical composition of pickled carp in contrast with fresh sample had changed except the water capacity.

经过剖杀、去头、清理、洗净和沥水后的鲢鱼按AOAC分析方法取样后，一部分进行新鲜样的水分含量、粗脂肪、粗蛋白质、粗灰份、TMA、pH、TVB-N和TBA的测定；剩下部分分别浸渍于空白和处理溶液中，鱼样和腌制液间的重量比在1：1，控制腌制温度在3±1℃，15天后对取自不同腌制液的鱼样分别进行水分含量、粗脂肪、粗蛋白质、灰份、氯化钠、感官、TMA、pH、TVB-N和TBA等项目的测定。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。