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The invention provides a ginkgo pyroligneous liquor and the application thereof in promoting the seed germination of crops and the growth of seedling, and the ginkgo pyroligneous liquor is prepared according to the following steps: after ginkgo wood is split into wood chips, the charring of ginkgo wood is carried out in a retort, the gas generated in the process of charring is condensed, the liquid after condensation is collected and then is placed still for three months, after that, supernatant liquid is separated, thus obtaining the ginkgo pyroligneous liquor.

本发明提供一种银杏木醋液及其在促进作物种子发芽和幼苗生长中的应用,所述银杏木醋液按如下步骤制备而成:将银杏木材劈成木片后,在干馏釜中进行银杏木材的炭化,冷凝炭化过程中产生的气体,收集冷凝后的液体,将收集到的液体静置3个月后分离出上层液体,得到银杏木醋液。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

Results The mortality rate of mice in 80 mg/kg, day cyclophosphamide group was 16.7%, and T level [ at 30th day :( 1.38 ± 0.31 );45th day:( 1.15 ± 0.26 ) ] and T/LH ratio [ at 30th day:(0.163 ± 0.014); 45th day:(0.127 ± 0.023 ) ] were significantly decreased (all P<0.05) at 30th day after induction;The concentration of MDA [at 15th day:(2.70 ± 0.41);30th day:(2.710.36);45th day:(2.67 ±0.43) ] was maintained at a high level (all P<0.05) during the 45 days ; Number of Leydig's cells [ at 15th day:(9.65 ± 0.75 ); 30th day:( 14.05 ± 0.67 ); 45th day:(8.49 ± 072)] and layers of spermatogenetic epithelia [ at 15th day:(4.75 ± 0.82);30th day:(3.60 ± 0.49);45th day:(3.74 ± 0.43 ) ] were significantly decreased ( all P < 0.01 ) and stabilized in a low level. The induced model was stable and the mortality rate was acceptable. In the 60 mg/kg, day cyelophosphamide group, the T level and T/LH ratio had no significant change (P > 0.05 ), and the concentration of MDA ,number of Leydig' s cell and layers of spermatogenetic epithelia recovered at 30th day after induction. The induced model was unstable.

结果 剂量每日为80 mg/kg体重小鼠成模后死亡率为16.7%,血清T[30 d:(1.38±0.31);45 d:(1.15±0.26)]及T/LH比值[30 d:(0.163±0.014);45 d:(0.127±0.023)]于诱导后第30天出现显著下降(P均<0.05),而诱导后睾丸组织内MDA含量[15 d:(2.70±0.41);30 d(2.71±0.36);45 d:(2.67±0.43)]维持高水平(P均<0.05),生精上皮层次[15 d:(4.75±0.82);30 d:(3.60±0.49);45 d:(3.74±0.43)]和间质细胞[15 d:(9.65±0.75);30 d:(14.05±0.67);45 d:(8.49±0.72)]均显著减少(P均<0.01)并稳定于低水平,模型稳定,死亡率适当;每日60mg/kg体重组小鼠血清T及T/LH比值于不同时段并未出现明显变化(P>0.05),且睾丸组织内MDA含量、生精上皮层次和间质细胞计数在30 d后有所恢复,模型不稳定;每日100 rag/ks体重组死亡率为30.0%,死亡率过高。

The first split of microspore mother cell is observed after one week after beginning of temperature arised under high temperature but the split of secondary sporogenous cell doesn't be found. The split of secondary sporogenous cell is still found after one week but the first split of microspore mother cell is observed after 12d under low temperature. Formating speed of male gametophyte is affected by temperature.

在高温条件下,开始加温1周后即看到小孢子母细胞进行第一次分裂,未观察到次生造孢细胞的分裂;在低温条件下,1周后仍能看到次生造孢细胞的分裂,12d后才看到小孢子母细胞进行第一次分裂。

Pristine Li-Al LDHs are synthesized by hydrothermal process in different reaction conditions by varying the aging time for 1 and 24 hours. Both the Li-Al layered double hydroxides (abbreviated as Li-Al LDH1 and Li-Al LDH24 for aging time 1 hour and 24 hours, respectively) were modified by using sulphanilic acid sodium salt hydrate, modified agent to form modified Li-Al LDH1-SAS and Li-Al LDH24-SAS. The morphology of the pristine layered double hydroxides are investigated by using wide angle X-rays diffraction, scanning electron microscopy and particle size analyzer which indicates that the crystallinity and aspect ratio is increased with increasing the aging time from 1 hour to 24 hours. The particle size of Li-Al LDH24 and Li-Al LDH1 are found to be ~1000 nm and ~250 nm, respectively. Both the pristine and modified LDHs are characterized by XRD, FTIR spectra and thermogravimetric analysis.

本研究目的在於改变长晶时间的长短,合成出同径大小的Li-Al LDHs,再经由改质剂sulphanilic acid sodium salt hydrate将Li-Al LDHs无机层材表面有机官能化制备出改质型Li-Al LDHs-SAS,於是进一步藉由扫描式电子显微镜、射径仪和穿透式电子显微镜等仪器分别观察LDHs改质前和改质后其主层结构型态上的变化;从中可以发现Li-Al LDHs随著长晶时间的增加其径有随之增大的趋势,但经有机化改质后其径会由於改质环境的影响明显低许多,针对此现象本实验将未改质和改质后之Li-Al人工无机层材制备成复材进一步探讨其在热性质和难燃特性上的为表现。

(1) Verification of the expressions of Cfos in caudate nucleus, putamen, globus pallidus, hippocamp, nuclei ventrolaterales thalami, parv ocellular reticular nucleus, dorso lateral magnocellular nucleus after rats subarachnoid hemorrhage are variation with different phases.(2) There are certain relationships between subarachnoid hemorrhage and different phases.

(1)大鼠蛛网膜下腔出血后不同时相Cfos在脑膜、脑皮质区及尾壳核、苍白球、海马、背侧丘脑腹后外侧核、小细胞网状核、背侧旁巨细胞核中都有表达且表达量随时相改变;(2)大鼠蛛网膜下腔出血后相关症状与不同时相Cfos在脑中表达之间存在着相关联系,具有规律性。

The result of different thawing temperatures indicated that the spermatozoa thawn in 38℃ water showed a better survival rate than those thawn in 50℃ water; the duration of preservation on above 30% survival rate were 4 and 1h, respectively.

结果显示:3%甘油冷冻解冻后精液的活率、活力(38.54%、52.24%)显著(P.05)高于2%甘油组(15.63%、28.22%),但3%甘油组顶体完整率(11.55%)显著(P.05)低于2%甘油组(21.71%)。38℃水浴解冻后的精子在39℃培养箱中4h内活率高于0.3,而50℃水浴解冻后1h后的活率低于0.3。

Over a 20-year period there was no increase in soil water content in the grazed grassland at depths of 2~9.9 m. There was a small increase(0.5~3.7 mm/a) in soil water content in the protected grassland, however the slow rate of increase suggests that at least 150 years would be needed for ungrazed grassland soils to regain water content similar to that present prior to planting trees. Recharge of deep soil moisture was faster in cropland(15 mm/a), but at this rate it would still take about 40 years to restore cropland soil water content to pre-plantation conditions.

结果表明,人工林死后的放牧荒坡在20a的时间里,其土壤水分没有补偿;人工林死后的保护草地土壤水分有微弱恢复迹象,但年恢复速度在0.5~3.7 mm之间,以这样的速度恢复到持续放牧荒坡的土壤含水量,至少需要150a以上;林后农地土壤含水量有恢复趋势,年平均恢复速度为15 mm左右,其土壤含水量要恢复到持续农地当前的水平,大约需要40a的时间。

So each theory says something unintuitive about the changes objects can and cannot survive: the just-matter theory says that persons can exist after rotting and disintegration; the takeover theory says ―― 140

所以,关于改变后客体能还是不能持存,两个理论都有些地方是不合乎直观:物块论说'人'在僵硬和消散后还能继续存在;接管论说泥块在有了更艺术的形状后就不再存在。

I lose and I gain; I make mistakes and I learn from them; I feel puzzled and I take a tumble...

我失去后获得,我犯错后增智,我困惑后顿悟,在库柏的每一天我都在成长。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。